PMID- 32632611
OWN - NLM
STAT- MEDLINE
DCOM- 20210317
LR  - 20240212
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 472
IP  - 1-2
DP  - 2020 Sep
TI  - Secreted frizzled-related protein 2 prevents pressure-overload-induced cardiac 
      hypertrophy by targeting the Wnt/beta-catenin pathway.
PG  - 241-251
LID - 10.1007/s11010-020-03802-x [doi]
AB  - BACKGROUND AND AIM: Secreted frizzled-related protein 2 (sFRP2) has been reported 
      to be involved in cardiovascular diseases. However, its role in cardiac 
      hypertrophy induced by pressure overload is still elusive. We aimed to examine 
      the role of sFRP2 in the development of cardiac hypertrophy in vivo and in vitro. 
      METHODS AND RESULTS: Following cardiac hypertrophy stimulated by aortic banding 
      (AB), the expression of sFRP2 was downregulated in the hypertrophic ventricle. 
      Adeno-associated virus 9 (AAV9) was injected through the tail vein to overexpress 
      sFRP2 in the mouse myocardium. Overexpression of sFRP2 alleviated cardiomyocyte 
      hypertrophy and interstitial fibrosis, as identified by the reduced cardiomyocyte 
      cross-sectional area, heart weight/body weight ratio, and left ventricular (LV) 
      collagen ratio. Additionally, sFRP2 decreased cardiomyocyte apoptosis induced by 
      pressure overload. Western blot showed that sFRP2 prevented the expression of 
      active beta-catenin. The Wnt/beta-catenin agonist LiCl (1 mmol/kg) abolished the 
      inhibitory effects of sFRP2 on cardiac hypertrophy and apoptosis, as evidenced by 
      the increased cross-sectional area and LV collagen ratio and the deterioration of 
      echocardiographic data. CONCLUSION: Our study indicated that decreased sFRP2 
      levels were observed in failing mouse hearts. Overexpression of sFRP2 attenuated 
      myocyte hypertrophy and interstitial fibrosis induced by hypertrophic stimuli by 
      inhibiting the Wnt/beta-catenin pathway. We revealed that sFRP2 may be a promising 
      therapeutic target for the development of cardiac remodeling.
FAU - Wei, Wen-Ying
AU  - Wei WY
AD  - Department of Intensive Care Unit, The Affiliated Hospital of Qingdao University, 
      Qingdao, China.
FAU - Zhao, Qing
AU  - Zhao Q
AD  - Department of Cardiology, The Affiliated Hospital of Qingdao University, No. 16 
      Jiangsu Road, Qingdao, 266000, People's Republic of China.
FAU - Zhang, Wen-Zhong
AU  - Zhang WZ
AD  - Department of Cardiology, The Affiliated Hospital of Qingdao University, No. 16 
      Jiangsu Road, Qingdao, 266000, People's Republic of China.
FAU - Wang, Mao-Jing
AU  - Wang MJ
AD  - Department of Cardiology, The Affiliated Hospital of Qingdao University, No. 16 
      Jiangsu Road, Qingdao, 266000, People's Republic of China.
FAU - Li, Yan
AU  - Li Y
AD  - Department of Cardiology, The Affiliated Hospital of Qingdao University, No. 16 
      Jiangsu Road, Qingdao, 266000, People's Republic of China.
FAU - Wang, Shi-Zhong
AU  - Wang SZ
AD  - Department of Cardiovascular Surgery, The Affiliated Hospital of Qingdao 
      University, Qingdao, China.
FAU - Zhang, Ning
AU  - Zhang N
AUID- ORCID: 0000-0003-1995-7251
AD  - Department of Cardiology, The Affiliated Hospital of Qingdao University, No. 16 
      Jiangsu Road, Qingdao, 266000, People's Republic of China. 
      zhangningqdfy@yeah.net.
LA  - eng
GR  - 3094/the Youth Fund of the Affiliated Hospital of Qingdao University/
PT  - Journal Article
DEP - 20200706
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Membrane Proteins)
RN  - 0 (Sfrp2 protein, mouse)
RN  - 0 (Sfrp2 protein, rat)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cardiomegaly/etiology/metabolism/pathology/*prevention & control
MH  - Disease Models, Animal
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pressure
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Wnt Proteins/genetics/*metabolism
MH  - beta Catenin/genetics/*metabolism
PMC - PMC7338134
OTO - NOTNLM
OT  - Apoptosis
OT  - Cardiac hypertrophy
OT  - Fibrosis
OT  - Pressure overload
OT  - Secreted frizzled-related protein 2 (sFRP2)
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/07/08 06:00
MHDA- 2021/03/18 06:00
PMCR- 2020/07/06
CRDT- 2020/07/08 06:00
PHST- 2019/11/15 00:00 [received]
PHST- 2020/06/18 00:00 [accepted]
PHST- 2020/07/08 06:00 [pubmed]
PHST- 2021/03/18 06:00 [medline]
PHST- 2020/07/08 06:00 [entrez]
PHST- 2020/07/06 00:00 [pmc-release]
AID - 10.1007/s11010-020-03802-x [pii]
AID - 3802 [pii]
AID - 10.1007/s11010-020-03802-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2020 Sep;472(1-2):241-251. doi: 10.1007/s11010-020-03802-x. 
      Epub 2020 Jul 6.