PMID- 32633057
OWN - NLM
STAT- MEDLINE
DCOM- 20210929
LR  - 20210929
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 14
IP  - 9
DP  - 2020 Sep
TI  - Control the fate of human umbilical cord mesenchymal stem cells with 
      dual-enzymatically cross-linked gelatin hydrogels for potential applications in 
      nerve regeneration.
PG  - 1261-1271
LID - 10.1002/term.3098 [doi]
AB  - Stem-cell-based therapy is a promising strategy to treat challenging neurological 
      diseases, while its application is hindered primarily by the low viability and 
      uncontrolled differentiation of stem cell. Hydrogel can be properly engineered to 
      share similar characteristics with the target tissue, thus promoting cell 
      viability and directing cell differentiation. In this study, we proposed a new 
      dual-enzymatically cross-linked and injectable gelatin hydrogel for regulating 
      survival, proliferation, and differentiation of human umbilical cord mesenchymal 
      stem cells (hUC-MSCs) in a three-dimensional matrix. This injectable gelatin 
      hydrogel was formed by oxidative coupling of gelatin-hydroxyphenyl acid 
      conjugates catalyzed by hydrogen horseradish peroxidase (HRP) and choline oxidase 
      (ChOx). Modulus and H(2) O(2) release can be well controlled by ChOx activity. 
      Results from calcein-AM/PI staining and Ki67 immunofluorescence tests 
      demonstrated that the survival and proliferation behavior of hUC-MSCs were highly 
      enhanced in HRP(1U) ChOx(0.25U) hydrogel with lower modulus and less H(2) O(2) 
      release compared with other groups. Attractively, the expression of 
      neuron-specific markers beta-III tubulin, neurofilament light chain (NFL), and 
      synapsin-1 was significantly increased in HRP(1U) ChOx(0.25U) hydrogel as well. 
      Additionally, in vitro hemolysis test and in vivo HE staining data highlighted 
      the good biocompatibility. Undoubtedly, this injectable gelatin hydrogel's 
      ability to control hUC-MSCs' fate holds enormous potentials in nervous disorders' 
      therapy and nerve regeneration.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Li, Jinrui
AU  - Li J
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
FAU - Gao, Feng
AU  - Gao F
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
FAU - Ma, Shanshan
AU  - Ma S
AUID- ORCID: 0000-0002-0780-4568
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
FAU - Zhang, Yanting
AU  - Zhang Y
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
FAU - Zhang, Junni
AU  - Zhang J
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
FAU - Guan, Fangxia
AU  - Guan F
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
FAU - Yao, Minghao
AU  - Yao M
AUID- ORCID: 0000-0002-2232-0991
AD  - School of Life Science, Zhengzhou University, Zhengzhou, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200713
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Biocompatible Materials)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Hydrogels)
RN  - 0 (Nerve Tissue Proteins)
RN  - 059QF0KO0R (Water)
RN  - 9000-70-8 (Gelatin)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/pharmacology
MH  - Cell Death/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cross-Linking Reagents/*pharmacology
MH  - Elastic Modulus
MH  - Gelatin/chemical synthesis/chemistry/*pharmacology
MH  - Hemolysis/drug effects
MH  - Humans
MH  - Hydrogels/*pharmacology
MH  - Hydrogen Peroxide/chemistry
MH  - Mesenchymal Stem Cells/*cytology
MH  - Mice, Inbred C57BL
MH  - *Nerve Regeneration/drug effects
MH  - Nerve Tissue Proteins/metabolism
MH  - Neurons/cytology/drug effects
MH  - Swine
MH  - Time Factors
MH  - Umbilical Cord/*cytology
MH  - Water/chemistry
OTO - NOTNLM
OT  - dual-enzymatically cross-linked gelatin hydrogels
OT  - human umbilical cord mesenchymal stem cells
OT  - neural differentiation
OT  - three-dimensional culture
EDAT- 2020/07/08 06:00
MHDA- 2021/09/30 06:00
CRDT- 2020/07/08 06:00
PHST- 2020/04/15 00:00 [received]
PHST- 2020/06/29 00:00 [revised]
PHST- 2020/07/01 00:00 [accepted]
PHST- 2020/07/08 06:00 [pubmed]
PHST- 2021/09/30 06:00 [medline]
PHST- 2020/07/08 06:00 [entrez]
AID - 10.1002/term.3098 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2020 Sep;14(9):1261-1271. doi: 10.1002/term.3098. Epub 
      2020 Jul 13.