PMID- 3263313
OWN - NLM
STAT- MEDLINE
DCOM- 19881222
LR  - 20181113
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 65
IP  - 1
DP  - 1988 Sep
TI  - A monoclonal antibody raised against a synthetic peptide representative of part 
      of the amino acid sequence of rat immunoglobulin E detects thermally induced 
      changes in that region of the IgE molecule.
PG  - 149-51
AB  - A mouse monoclonal antibody (mAb) has been produced by conventional cell fusion 
      methods against a synthetic peptide, p123, representative of a portion of the CH4 
      domain of rat immunoglobulin E (IgE). This monoclonal antibody was reactive with 
      both peptide and purified rat IgE (p.rat IgE) by indirect enzyme immunosorbent 
      assay (ELISA), and its binding to p.rat IgE was found to be inhibitable by 
      pre-incubation with rat ascitic fluid containing the immunocytoma 162 (IR162) 
      IgE. Heating of the immunocytoma IgE in solution at 56 degrees for 1 hr resulted 
      in its enhanced binding of the mAb. The effect of this treatment was investigated 
      further using p.rat IgE heated at 56 degrees for various time intervals between 0 
      and 60 min. The mAb showed enhanced binding to IgE heated for as little as 10 
      min, a similar level of binding being shown by samples heated for 30 and 60 min. 
      The degree of aggregation of the IgE molecules brought about by the heat 
      treatments was measured by differential UV absorption. This revealed a decrease 
      in the proportion of monomeric IgE with an accompanying increase in the 
      percentage of dimer and larger aggregates with increased time of heating at 56 
      degrees. These absorption data, together with the ELISA inhibition data, suggest 
      that, rather than inducing changes mediated by aggregation of the IgE molecules 
      in solution, heating at 56 degrees causes subtle alterations in the conformation 
      of individual IgE molecules at specific sites within their CH4 domains, one of 
      which is detected by this mAb.
FAU - Hastings, G Z
AU  - Hastings GZ
AD  - Department of Immunology, University of Birmingham, U.K.
FAU - Burt, D S
AU  - Burt DS
FAU - Stanworth, D R
AU  - Stanworth DR
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Peptide Fragments)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/immunology
MH  - Antibodies, Monoclonal/immunology
MH  - Binding, Competitive
MH  - *Hot Temperature
MH  - Immunoglobulin E/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Peptide Fragments/*immunology
MH  - Protein Denaturation
PMC - PMC1385034
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
PMCR- 1989/09/01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
PHST- 1989/09/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1988 Sep;65(1):149-51.