PMID- 32634256 OWN - NLM STAT- MEDLINE DCOM- 20210823 LR - 20210823 IS - 1365-2559 (Electronic) IS - 0309-0167 (Linking) VI - 77 IP - 6 DP - 2020 Dec TI - Immunohistological expression of oestrogen receptor, progesterone receptor, mammaglobin, human epidermal growth factor receptor 2 and GATA-binding protein 3 in non-small-cell lung cancer. PG - 900-914 LID - 10.1111/his.14203 [doi] AB - AIMS: Non-small-cell lung cancer (NSCLC) and breast cancer are common entities. Staining for oestrogen receptor (ER), progesterone receptor (PgR), mammaglobin (MAMG) and GATA-binding protein 3 (GATA3) is frequently performed to confirm a mammary origin in the appropriate diagnostic setting. However, comprehensive data on the immunohistological expression of these markers in NSCLC are limited. Therefore, the aim of this study was to analyse a large cohort of NSCLCs and correlate the staining results with clinicopathological variables. METHODS AND RESULTS: A tissue microarray was stained for ER, PgR, MAMG, human epidermal growth factor receptor 2 (HER2), and GATA3, and included 636 adenocarcinomas (ADCs), 536 squamous cell carcinomas (SqCCs), 65 large-cell-carcinomas, 34 pleomorphic carcinomas, and 20 large-cell neuroendocrine carcinomas. HER2 status was determined for immunohistochemically positive cases with chromogenic in-situ hybridisation. Markers with a proportion of >/=5% positive cases in ADC and SqCC were considered for survival analysis. Among ADCs, 62 (10%), 17 (3%), one (<1%), seven (1%), and 49 (8%) cases were positive for ER, PgR, MAMG, HER2, and GATA3, respectively. Among SqCCs, 10 (2%), 14 (3%), two (<1%) and 109 (20%) cases were positive for ER, PgR, HER2, and GATA3, but none of the samples showed positivity for MAMG. ER positivity was associated with ADC, female sex, smaller tumour size, and lower clinical stage. None of the markers had an impact on survival. CONCLUSION: We report on ER, PgR, MAMG, HER2 and GATA3 expression in a large cohort of NSCLCs. Interpretation of these markers in the differential diagnostic setting should be based on a multimarker panel. CI - (c) 2020 The Authors. Histopathology published by John Wiley & Sons Ltd. FAU - Kriegsmann, Katharina AU - Kriegsmann K AD - Department of Internal Medicine V, Haematology, Oncology and Rheumatology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Zgorzelski, Christiane AU - Zgorzelski C AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Muley, Thomas AU - Muley T AD - Translational Lung Research Centre Heidelberg, Member of the German Centre for Lung Research (DZL), Thoraxklinik at Heidelberg University, Heidelberg, Germany. AD - Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany. FAU - Christopoulos, Petros AU - Christopoulos P AD - Department of Thoracic Oncology, Thoraxklinik at Heidelberg University, Heidelberg, Germany. FAU - von Winterfeld, Moritz AU - von Winterfeld M AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Herpel, Esther AU - Herpel E AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Goeppert, Benjamin AU - Goeppert B AUID- ORCID: 0000-0002-4135-9250 AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Mechtersheimer, Gunhild AU - Mechtersheimer G AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Sinn, Peter AU - Sinn P AUID- ORCID: 0000-0003-2836-6699 AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Stenzinger, Albrecht AU - Stenzinger A AUID- ORCID: 0000-0003-1001-103X AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. AD - Translational Lung Research Centre Heidelberg, Member of the German Centre for Lung Research (DZL), Thoraxklinik at Heidelberg University, Heidelberg, Germany. FAU - Schirmacher, Peter AU - Schirmacher P AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Winter, Hauke AU - Winter H AD - Translational Lung Research Centre Heidelberg, Member of the German Centre for Lung Research (DZL), Thoraxklinik at Heidelberg University, Heidelberg, Germany. AD - Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany. AD - Department of Thoracic Surgery, Thoraxklinik at Heidelberg University, Heidelberg, Germany. FAU - Eichinger, Monika AU - Eichinger M AD - Department of Radiology, Thoraxklinik at Heidelberg University, Heidelberg, Germany. FAU - Warth, Arne AU - Warth A AD - Institute of Pathology, Cytopathology, and Molecular Pathology, UEGP MVZ Giessen/Wetzlar/Limburg, Limburg, Germany. FAU - Kriegsmann, Mark AU - Kriegsmann M AUID- ORCID: 0000-0002-7319-3646 AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. AD - Translational Lung Research Centre Heidelberg, Member of the German Centre for Lung Research (DZL), Thoraxklinik at Heidelberg University, Heidelberg, Germany. AD - Translational Research Unit, Thoraxklinik at Heidelberg University, Heidelberg, Germany. LA - eng PT - Journal Article DEP - 20201011 PL - England TA - Histopathology JT - Histopathology JID - 7704136 RN - 0 (Biomarkers, Tumor) RN - 0 (GATA3 Transcription Factor) RN - 0 (GATA3 protein, human) RN - 0 (Mammaglobin A) RN - 0 (Receptors, Progesterone) RN - EC 2.7.10.1 (ERBB2 protein, human) RN - EC 2.7.10.1 (Receptor, ErbB-2) SB - IM MH - Adenocarcinoma/diagnosis/metabolism/pathology MH - Adult MH - Aged MH - Aged, 80 and over MH - Biomarkers, Tumor/metabolism MH - Carcinoma, Large Cell/diagnosis/metabolism/pathology MH - Carcinoma, Neuroendocrine/diagnosis/metabolism/pathology MH - Carcinoma, Non-Small-Cell Lung/diagnosis/*metabolism/pathology MH - Diagnosis, Differential MH - Female MH - GATA3 Transcription Factor/*metabolism MH - Humans MH - Immunohistochemistry MH - Lung Neoplasms/metabolism/pathology MH - Mammaglobin A/*metabolism MH - Middle Aged MH - Receptor, ErbB-2/*metabolism MH - Receptors, Progesterone/*metabolism MH - Tissue Array Analysis OTO - NOTNLM OT - ER OT - GATA3 OT - HER2 OT - NSCLC OT - PgR OT - lung cancer OT - mammaglobin EDAT- 2020/07/08 06:00 MHDA- 2021/08/24 06:00 CRDT- 2020/07/08 06:00 PHST- 2020/04/20 00:00 [received] PHST- 2020/06/27 00:00 [revised] PHST- 2020/07/02 00:00 [accepted] PHST- 2020/07/08 06:00 [pubmed] PHST- 2021/08/24 06:00 [medline] PHST- 2020/07/08 06:00 [entrez] AID - 10.1111/his.14203 [doi] PST - ppublish SO - Histopathology. 2020 Dec;77(6):900-914. doi: 10.1111/his.14203. Epub 2020 Oct 11.