PMID- 32634322
OWN - NLM
STAT- MEDLINE
DCOM- 20210809
LR  - 20210809
IS  - 1465-3338 (Electronic)
IS  - 0307-9457 (Linking)
VI  - 49
IP  - 6
DP  - 2020 Dec
TI  - Riemerella anatipestifer infection affects intestinal barrier structure and 
      immune reactions in the duck caecum.
PG  - 572-580
LID - 10.1080/03079457.2020.1792414 [doi]
AB  - Riemerella anatipestifer (RA) infection causes high mortality and poor feed 
      conversion, leading to great economic losses to the duck industry. This study 
      investigated the effects of RA on the intestinal morphology and immune response 
      of ducks. Histological examination showed that RA infection caused intestinal 
      injury, including significantly reduced mucosal thickness on days 2, 3 and 5, 
      significantly reduced villus height on days 1, 2, 3 and 5 (P < 0.05) and 
      significantly reduced villus height to crypt depth ratios on days 2, 3, 5 and 9 
      of RA infection (P < 0.05). The expression of intestinal mucosal layer 
      construction-associated genes and tight junction genes was significantly altered 
      on at least one time point (day 1, 2, 3, 5, 9 or 14) after RA infection. 
      Quantitative real-time polymerase chain reaction revealed that RA infection 
      affected intestinal mucosal immune function. The genes encoding TLR4 (toll like 
      receptor-4), TRAF6 (TNF receptor-associated factor 6), MYD88 (myeloid 
      differentiation factor 88), IFN-gamma (interferon-gamma), IL (interleukin)-4 and IL-8 
      were significantly upregulated on day 2 of RA infection. Taken together, these 
      results indicate that RA infection negatively affects intestinal barrier function 
      in ducks due to impaired mucosal and villus-crypt structure and alters the mRNA 
      expression of mucous layer construction-, intestinal tight junction-, and 
      intestinal mucosal immunity-related genes.
FAU - Tao, Zhiyun
AU  - Tao Z
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infections Diseases and Zoonoses, Yangzhou, People's Republic of China.
FAU - Zhu, Chunhong
AU  - Zhu C
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infections Diseases and Zoonoses, Yangzhou, People's Republic of China.
FAU - Xu, Wenjuan
AU  - Xu W
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
FAU - Shi, Zuhao
AU  - Shi Z
AD  - Pony Testing Group Jiangsu Co., Ltd, Suzhou, People's Republic of China.
FAU - Zhang, Shuangjie
AU  - Zhang S
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
FAU - Song, Weitao
AU  - Song W
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
FAU - Liu, Hongxiang
AU  - Liu H
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
FAU - Li, Huifang
AU  - Li H
AD  - Department of Waterfowl Breeding and Production, Jiangsu Institute of Poultry 
      Science, Yangzhou, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20200804
PL  - England
TA  - Avian Pathol
JT  - Avian pathology : journal of the W.V.P.A
JID - 8210638
RN  - 0 (RNA, Messenger)
RN  - Riemerella anatipestifer
SB  - IM
MH  - Animals
MH  - Cecum/immunology/microbiology/pathology
MH  - Ducks/*immunology/virology
MH  - Flavobacteriaceae Infections/immunology/pathology/*veterinary
MH  - *Immunity
MH  - Intestinal Mucosa/immunology/microbiology
MH  - Male
MH  - Poultry Diseases/immunology/*pathology
MH  - RNA, Messenger/genetics
MH  - Random Allocation
MH  - Riemerella/*immunology
OTO - NOTNLM
OT  - Riemerella anatipestifer
OT  - duck
OT  - immune reaction
OT  - intestinal morphology
EDAT- 2020/07/08 06:00
MHDA- 2021/08/10 06:00
CRDT- 2020/07/08 06:00
PHST- 2020/07/08 06:00 [pubmed]
PHST- 2021/08/10 06:00 [medline]
PHST- 2020/07/08 06:00 [entrez]
AID - 10.1080/03079457.2020.1792414 [doi]
PST - ppublish
SO  - Avian Pathol. 2020 Dec;49(6):572-580. doi: 10.1080/03079457.2020.1792414. Epub 
      2020 Aug 4.