PMID- 32638385
OWN - NLM
STAT- MEDLINE
DCOM- 20210416
LR  - 20210416
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 147
IP  - 10
DP  - 2020 Nov 15
TI  - Cellular heterogeneity landscape in laryngeal squamous cell carcinoma.
PG  - 2879-2890
LID - 10.1002/ijc.33192 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a highly malignant tumor originated 
      from respiratory system. Although there have been many improvements in therapy 
      until now, reducing the high mortality remains difficult. Understanding the 
      cellular heterogeneity of LSCC could contribute to improve this problem. 
      Single-cell RNA sequencing was applied to dissect the cell composition and 
      molecular characteristics of LSCC tissues. Immunohistochemistry staining of the 
      LSCC tissues was performed to identify the spatial location of tumor cells. 
      Survival analysis of marker genes was executed in The Cancer Genome Atlas to 
      verify the correlation between each cell clusters and patients' prognosis. The 
      LSCC tissue cells were finely grouped into various clusters, including tumor 
      cells, immune cells, epithelial cells, fibroblasts and endothelial cells. 
      Notably, in tumor cells, keratinocyte-like cells were in the core of tumor while 
      malignant proliferating cells were located at the tumor edge. The malignant 
      proliferating cells were correlated with poor prognosis. In summary, this is the 
      first study to delineate a landscape of the LSCC intratumor heterogeneity. Our 
      work might help researchers have a better understanding for tumor progression.
CI  - (c) 2020 UICC.
FAU - Song, Lianhao
AU  - Song L
AUID- ORCID: 0000-0002-3211-3726
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
FAU - Zhang, Siwei
AU  - Zhang S
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
FAU - Yu, Siyang
AU  - Yu S
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
FAU - Ma, Fen
AU  - Ma F
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
FAU - Wang, Bozhi
AU  - Wang B
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
FAU - Zhang, Congcong
AU  - Zhang C
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
FAU - Sun, Ji
AU  - Sun J
AD  - Department of Otolaryngology, Head and Neck Surgery, Harbin Medical University 
      Cancer Hospital, Harbin, China.
FAU - Mao, Xionghui
AU  - Mao X
AD  - Department of Otolaryngology, Head and Neck Surgery, Harbin Medical University 
      Cancer Hospital, Harbin, China.
FAU - Wei, Lanlan
AU  - Wei L
AUID- ORCID: 0000-0002-6060-4303
AD  - Department of Microbiology, Harbin Medical University, Harbin, China.
AD  - Wu Lien-Teh Institute, Harbin Medical University, Harbin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200731
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Genetic Heterogeneity
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Sequence Analysis, RNA/*methods
MH  - Single-Cell Analysis/*methods
MH  - Survival Analysis
OTO - NOTNLM
OT  - biomarkers
OT  - intratumor heterogeneity
OT  - laryngeal squamous cell carcinoma
OT  - single-cell sequencing
EDAT- 2020/07/09 06:00
MHDA- 2021/04/17 06:00
CRDT- 2020/07/09 06:00
PHST- 2020/02/17 00:00 [received]
PHST- 2020/06/24 00:00 [revised]
PHST- 2020/06/29 00:00 [accepted]
PHST- 2020/07/09 06:00 [pubmed]
PHST- 2021/04/17 06:00 [medline]
PHST- 2020/07/09 06:00 [entrez]
AID - 10.1002/ijc.33192 [doi]
PST - ppublish
SO  - Int J Cancer. 2020 Nov 15;147(10):2879-2890. doi: 10.1002/ijc.33192. Epub 2020 
      Jul 31.