PMID- 3263974
OWN - NLM
STAT- MEDLINE
DCOM- 19890112
LR  - 20131121
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 137
IP  - 3
DP  - 1988 Dec
TI  - Adenosine induction of rapid catabolism of adenine ribonucleotides and 
      independent elevation of the ATP content in quiescent mouse fibroblasts.
PG  - 565-70
AB  - The influence of adenosine on the ribonucleotide metabolism in quiescent BALB/c 
      3T3 cells was studied. The cellular adenine ribonucleotides were labelled by 
      pretreating the cells with [2-3H]-adenine. After addition of adenosine to the 
      cell cultures, the amount and radioactivity of the cellular purine 
      ribonucleotides and the radioactivity of the purine compounds in the medium were 
      determined. It appeared that adenosine gave rise both to rapid catabolism of 
      adenine ribonucleotides with inosine 5'-monophosphate (IMP) as an intermediate 
      and to expansion of the cellular adenosine 5'-triphosphate (ATP) pool. The 
      maximal rates and the apparent activation constants for the two processes have 
      been determined. Experiments with varying concentrations of coformycin (an 
      inhibitor of adenosine 5'-monophosphate [AMP] deaminase and adenosine deaminase) 
      and of 5'-amino-5'-deoxyadenosine (an inhibitor of adenosine kinase), 
      respectively, showed that each compound may almost completely inhibit the 
      adenosine-induced catabolism. This effect can be obtained under conditions where 
      there was little or no effect by the two inhibitors on the rate of expansion of 
      the cellular ATP pool. These results may best be explained by assuming that the 
      process of expansion of the ATP pool is independent of the induced catabolism of 
      adenine ribonucleotides, even though both processes seem to depend on the 
      phosphorylation of adenosine to AMP. The total increase in the pool size of ATP 
      and of guanosine 5'-triphosphate (GTP), both caused by adenosine, seems not to 
      have regulatory effect on adenine ribonucleotide catabolism.
FAU - Klenow, H
AU  - Klenow H
AD  - Biokemisk Institut B, Panum Instituttet, University of Copenhagen, Denmark.
FAU - Ostergaard, E
AU  - Ostergaard E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Deoxyadenosines)
RN  - 11033-22-0 (Coformycin)
RN  - 131-99-7 (Inosine Monophosphate)
RN  - 14365-44-7 (5'-amino-5'-deoxyadenosine)
RN  - 415SHH325A (Adenosine Monophosphate)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine/*pharmacology
MH  - Adenosine Diphosphate/*metabolism
MH  - Adenosine Monophosphate/*metabolism
MH  - Adenosine Triphosphate/*metabolism
MH  - Animals
MH  - Cell Line
MH  - Coformycin/pharmacology
MH  - Deoxyadenosines/analogs & derivatives/pharmacology
MH  - Guanosine Triphosphate/metabolism
MH  - Inosine Monophosphate/metabolism
MH  - *Interphase
MH  - Kinetics
MH  - Mice
EDAT- 1988/12/01 00:00
MHDA- 1988/12/01 00:01
CRDT- 1988/12/01 00:00
PHST- 1988/12/01 00:00 [pubmed]
PHST- 1988/12/01 00:01 [medline]
PHST- 1988/12/01 00:00 [entrez]
AID - 10.1002/jcp.1041370324 [doi]
PST - ppublish
SO  - J Cell Physiol. 1988 Dec;137(3):565-70. doi: 10.1002/jcp.1041370324.