PMID- 32640423
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210308
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 12
IP  - 13
DP  - 2020 Jul 8
TI  - TRIM29 mediates lung squamous cell carcinoma cell metastasis by regulating 
      autophagic degradation of E-cadherin.
PG  - 13488-13501
LID - 10.18632/aging.103451 [doi]
AB  - Lung squamous cell carcinoma (LSCC) is the most common histological type of 
      primary lung cancer. In this study, we had tested the biological role of TRIM29 
      in LSCC cells. TRIM29 abundance, the relationships between TRIM29 and E-cadherin 
      and autophagy degradation related proteins in clinical tissues and six cell lines 
      were studied with quantitative real-time PCR test (qRT-PCR) and western blot. 
      TRIM29 overexpression treated HTB-182 cells and knockdown treated NCL-H1915 cells 
      was used for studying cell proliferation, colony formation, migration, invasion, 
      and the expression of epithelial mesenchymal transformation (EMT) associated 
      biomarkers. The relationships between TRIM29 and BECN1 were investigated with 
      western blot. TRIM29 was profoundly overexpressed in LSCC tissues and cells 
      compared with human normal bronchial epithelial cells (HNBE). High TRIM29 
      expression was closely related to overall survival (OS). TRIM29 overexpression 
      and knockdown affected LSCC activity and the expression of EMT associated 
      biomarkers. TRIM29 can regulate the degradation of E-cadherin and autophagy of 
      LSCC through BECN1 gene, and promote autophagy in HTB-182 and NCL-H1915 cells. 
      Our results revealed that TRIM29 could promote the proliferation, migration, and 
      invasion of LSCC via E-cadherin autophagy degradation. The results are useful for 
      further study in LSCC.
FAU - Xu, Weifeng
AU  - Xu W
AD  - Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou 
      University, Zhengzhou 450008, Henan, P.R. China.
FAU - Chen, Beibei
AU  - Chen B
AD  - Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou 
      University, Zhengzhou 450008, Henan, P.R. China.
FAU - Ke, Dianshan
AU  - Ke D
AD  - Department of Cell Biology, Southern Medical University, Guangzhou 510515, 
      Guangdong, China.
FAU - Chen, Xiaobing
AU  - Chen X
AD  - Department of Medical Oncology, The Affiliated Cancer Hospital of Zhengzhou 
      University, Zhengzhou 450008, Henan, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Observational Study
DEP - 20200708
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Antigens, CD)
RN  - 0 (CDH1 protein, human)
RN  - 0 (Cadherins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (TRIM29 protein, human)
RN  - 0 (Transcription Factors)
SB  - IM
EIN - Aging (Albany NY). 2020 Jul 30;12(14):15183. PMID: 32756016
MH  - Antigens, CD/*metabolism
MH  - Autophagy/*genetics
MH  - Cadherins/*metabolism
MH  - Carcinoma, Squamous Cell/genetics/mortality/*secondary
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Datasets as Topic
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Lung/pathology
MH  - Lung Neoplasms/genetics/mortality/*pathology
MH  - Neoplasm Invasiveness/genetics
MH  - Proteolysis
MH  - Transcription Factors/genetics/*metabolism
PMC - PMC7377877
OTO - NOTNLM
OT  - EMT
OT  - HNBE
OT  - LSCC
OT  - OS
OT  - TRIM29
COIS- CONFLICTS OF INTEREST: Authors declare no conflicts of interest.
EDAT- 2020/07/09 06:00
MHDA- 2021/03/09 06:00
PMCR- 2020/07/15
CRDT- 2020/07/09 06:00
PHST- 2019/12/21 00:00 [received]
PHST- 2020/05/01 00:00 [accepted]
PHST- 2020/07/09 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/07/09 06:00 [entrez]
PHST- 2020/07/15 00:00 [pmc-release]
AID - 103451 [pii]
AID - 10.18632/aging.103451 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2020 Jul 8;12(13):13488-13501. doi: 10.18632/aging.103451. 
      Epub 2020 Jul 8.