PMID- 32641145
OWN - NLM
STAT- MEDLINE
DCOM- 20210408
LR  - 20210408
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 17
IP  - 1
DP  - 2020 Jul 8
TI  - Longitudinal transcriptomic characterization of viral genes in HSV-1 infected 
      tree shrew trigeminal ganglia.
PG  - 95
LID - 10.1186/s12985-020-01344-8 [doi]
LID - 95
AB  - BACKGROUND: Following acute infection, Herpes Simplex virus-1 (HSV-1) establishes 
      lifelong latency and recurrent reactivation in the sensory neurons of trigeminal 
      ganglia (TG). Infected tree shrew differs from mouse and show characteristics 
      similar to human infection. A detailed transcriptomic analysis of the tree shrew 
      model could provide mechanistic insights into HSV-1 infection in humans. METHODS: 
      We sequenced the transcriptome of infected TGs from tree shrews and mice, and 4 
      human donors, then examined viral genes expression up to 58 days in infected TGs 
      from mouse and tree shrew, and compare the latency data with that in human TGs. 
      RESULTS: Here, we found that all HSV-1 genes could be detected in mouse TGs 
      during acute infection, but 22 viral genes necessary for viral transcription, 
      replication and viral maturation were not expressed in tree shrew TGs during this 
      stage. Importantly, during latency, we found that LAT could be detected both in 
      mouse and tree shrew, but the latter also has an ICP0 transcript signal absent in 
      mouse but present in human samples. Importantly, we observed that infected human 
      and tree shrew TGs have a more similar LAT region transcription peak. More 
      importantly, we observed that HSV-1 spontaneously reactivates from latently 
      infected tree shrews with relatively high efficiency. CONCLUSIONS: These results 
      represent the first longitudinal transcriptomic characterization of HSV-1 
      infection in during acute, latency and recurrent phases, and revealed that tree 
      shrew infection has important similar features with human infection.
FAU - Wang, Erlin
AU  - Wang E
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China.
AD  - Kunming College of Life Science, University of Chinese Academy of Sciences, 
      Beijing, 100049, China.
FAU - Ye, Yunshuang
AU  - Ye Y
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China.
AD  - Kunming College of Life Science, University of Chinese Academy of Sciences, 
      Beijing, 100049, China.
FAU - Zhang, Ke
AU  - Zhang K
AD  - Key Laboratory of Second Affiliated Hospital of Kunming Medical University, 
      Kunming, 650000, Yunnan, China.
AD  - Department of medicine laboratory, Fuwai Central China Cardiovascular Hospital, 
      Zhengzhou, 450003, Henan, China.
FAU - Yang, Jinlong
AU  - Yang J
AD  - BGI-Yunnan, BGI-Shenzhen, Kunming, 650000, Yunnan, China.
AD  - College of Forensic Science, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, 
      China.
FAU - Gong, Daohua
AU  - Gong D
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China.
AD  - Kunming College of Life Science, University of Chinese Academy of Sciences, 
      Beijing, 100049, China.
FAU - Zhang, Jianhua
AU  - Zhang J
AD  - Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, 
      Academy of Forensic Science, Shanghai, 200063, China.
FAU - Hong, Renjun
AU  - Hong R
AD  - School of Forensic Medicine, Kunming Medical University, Kunming, 650101, Yunnan, 
      China.
FAU - Zhang, Huan
AU  - Zhang H
AD  - School of Forensic Medicine, Kunming Medical University, Kunming, 650101, Yunnan, 
      China.
FAU - Li, Lihong
AU  - Li L
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China.
FAU - Chen, Guijun
AU  - Chen G
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China.
FAU - Yang, Liping
AU  - Yang L
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China.
FAU - Liu, Jianmei
AU  - Liu J
AD  - BGI-Yunnan, BGI-Shenzhen, Kunming, 650000, Yunnan, China.
FAU - Cao, Hanyu
AU  - Cao H
AD  - Key Laboratory of Second Affiliated Hospital of Kunming Medical University, 
      Kunming, 650000, Yunnan, China.
FAU - Du, Ting
AU  - Du T
AD  - Key Laboratory of Second Affiliated Hospital of Kunming Medical University, 
      Kunming, 650000, Yunnan, China.
FAU - Fraser, Nigel W
AU  - Fraser NW
AD  - Department of Microbiology, Perelman School of Medicine, University of 
      Pennsylvania, Philadelphia, PA19104, USA.
FAU - Cheng, Le
AU  - Cheng L
AD  - BGI-Yunnan, BGI-Shenzhen, Kunming, 650000, Yunnan, China. chengle@bgi.com.
FAU - Cao, Xia
AU  - Cao X
AD  - Key Laboratory of Second Affiliated Hospital of Kunming Medical University, 
      Kunming, 650000, Yunnan, China. 1244815508@qq.com.
FAU - Zhou, Jumin
AU  - Zhou J
AD  - Key Laboratory of Animal Models and Human Disease Mechanism of the Chinese 
      Academy of Science/Key Laboratory of Healthy Aging Research of Yunnan Province, 
      Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, 650223, 
      Yunnan, China. zhoujm@mail.kiz.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200708
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Viral Proteins)
SB  - IM
EIN - Virol J. 2020 Oct 8;17(1):150. PMID: 33032632
MH  - Acute Disease
MH  - Adult
MH  - Animals
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - *Genes, Viral
MH  - Herpes Simplex/*veterinary/virology
MH  - Herpesvirus 1, Human/*genetics/physiology
MH  - Humans
MH  - Longitudinal Studies
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - RNA-Seq
MH  - *Transcriptome
MH  - Trigeminal Ganglion/*virology
MH  - Tupaiidae/*virology
MH  - Viral Proteins/genetics
MH  - Virus Latency
MH  - Virus Replication
PMC - PMC7341572
OTO - NOTNLM
OT  - HSV-1
OT  - Latency
OT  - Longitudinal study
OT  - RNAseq
OT  - Spontaneous reactivation
OT  - Transcriptome
OT  - Tree shrew
OT  - Trigeminal ganglia
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2020/07/10 06:00
MHDA- 2021/04/10 06:00
PMCR- 2020/07/08
CRDT- 2020/07/10 06:00
PHST- 2020/04/09 00:00 [received]
PHST- 2020/06/01 00:00 [accepted]
PHST- 2020/07/10 06:00 [entrez]
PHST- 2020/07/10 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
PHST- 2020/07/08 00:00 [pmc-release]
AID - 10.1186/s12985-020-01344-8 [pii]
AID - 1344 [pii]
AID - 10.1186/s12985-020-01344-8 [doi]
PST - epublish
SO  - Virol J. 2020 Jul 8;17(1):95. doi: 10.1186/s12985-020-01344-8.