PMID- 3264365
OWN - NLM
STAT- MEDLINE
DCOM- 19890126
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 2
IP  - 12 Suppl
DP  - 1988 Dec
TI  - Chromosome damage induced in cord blood T-lymphocytes infected in vitro by 
      HTLV-I.
PG  - 223S-232S
AB  - Experiments were carried out to investigate whether the human T-lymphotropic 
      virus type I (HTLV-I), alone or in combination with a chemical mutagen such as 
      mitomycin C (MMC), has the capacity to damage host chromosomes. Cord-blood T 
      lymphocytes (CBL) were infected by co-cultivation with lethally irradiated 
      HTLV-I-producing cells. Infected and immortalized CBL were then studied for 
      frequencies of sister chromatid exchanges (SCE), chromosome breaks and 
      micronuclei. HTLV-I-infected cells had statistically higher baseline SCE, 
      chromosome aberrations and micronucleus values than the uninfected control CBL. 
      While MMC treatment further augmented these values both in control and in 
      infected lymphocytes, the latter did not show dose-related increases, most likely 
      because of the more pronounced MMC-induced delaying effect on cell progression to 
      mitosis. In view of similar previous observations in mouse lymphocytes carrying 
      the Moloney murine leukemia virus, it is suggested that expression of a common 
      retrovirus gene product, such as the pol endonuclease, might be responsible for 
      the cytogenetic abnormalities observed. In addition to the IL-2 autocrine loop, 
      the direct induction of chromosome damage by HTLV-I in target lymphocytes may be 
      related to the pathogenesis of malignancies associated with HTLV-I infection.
FAU - Chieco-Bianchi, L
AU  - Chieco-Bianchi L
AD  - Institute of Oncology, University of Padova, Italy.
FAU - Saggioro, D
AU  - Saggioro D
FAU - Del Mistro, A
AU  - Del Mistro A
FAU - Montaldo, A
AU  - Montaldo A
FAU - Majone, F
AU  - Majone F
FAU - Levis, A G
AU  - Levis AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Cell Transformation, Viral
MH  - Chromosome Aberrations
MH  - Chromosomes, Human/*ultrastructure
MH  - Fetal Blood/cytology
MH  - Human T-lymphotropic virus 1/*physiology
MH  - Humans
MH  - Micronucleus Tests
MH  - Mutagenicity Tests
MH  - Sister Chromatid Exchange
MH  - T-Lymphocytes/microbiology/*ultrastructure
EDAT- 1988/12/01 00:00
MHDA- 1988/12/01 00:01
CRDT- 1988/12/01 00:00
PHST- 1988/12/01 00:00 [pubmed]
PHST- 1988/12/01 00:01 [medline]
PHST- 1988/12/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1988 Dec;2(12 Suppl):223S-232S.