PMID- 32645537 OWN - NLM STAT- MEDLINE DCOM- 20211116 LR - 20211116 IS - 1096-0023 (Electronic) IS - 1043-4666 (Linking) VI - 134 DP - 2020 Oct TI - The high expression of the pain-related inflammatory factors in the eyes of cataract patients infected with hepatitis B virus. PG - 155189 LID - S1043-4666(20)30205-2 [pii] LID - 10.1016/j.cyto.2020.155189 [doi] AB - In cataract surgery, it is often found that patients infected hepatitis B virus (HBV) are likely to suffer from more pain than other patients. In order to assess the inflammation status of the aqueous humor in the eyes of cataract patients infected with HBV. RayBio Human Inflammation Array was used to assay aqueous humor samples collected from 14 eyes of patients infected with HBV and 14 eyes of cataract patients without HBV infection (the controls) during the cataract surgery. RayBio Human Quantibody Cutom Array was adopted for the validation of the screened cytokines, with aqueous humor samples collected from 40 eyes of patients infected with HBV and 40 eyes of the controls. A pain questionnaire survey about the surgery was conducted in all patients after operation. The results of questionnaire showed that patients infected with HBV were more likely to have pain during operation. The Human Inflammation Array revealed that the expression levels of monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor alpha (TNF-alpha) and tumor necrosis factor beta (TNF-beta) were very high in HBV infected patients and IL-1ra was much lower in patients infected with HBV (all, P < 0.05). In validation, the Human Quantibody Cutom Array revealed that the expression levels of MCP-1 and TNF-alpha were high in HBV infected patients with significant difference (all P < 0.05). These results revealed that pain-related inflammatory factors MCP-1 and TNF-alpha were increased in aqueous humor of cataract patients infected with HBV, which indicates that patients infected with HBV may be more prone to intraoperative pain. CI - Copyright (c) 2020 Elsevier Ltd. All rights reserved. FAU - Zhang, Jing Shang AU - Zhang JS AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Wang, Jin Da AU - Wang JD AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Zhu, Gu Yu AU - Zhu GY AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Yusufu, Mayinuer AU - Yusufu M AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Xiong, Ying AU - Xiong Y AD - Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100005, China. FAU - Li, Jing AU - Li J AD - Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100005, China. FAU - Cao, Kai AU - Cao K AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Jin, Shan Shan AU - Jin SS AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - He, Hai Long AU - He HL AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Sun, Xiu Li AU - Sun XL AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. FAU - Wan, Xiu Hua AU - Wan XH AD - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing 100005, China. Electronic address: xiuhuawan@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20200706 PL - England TA - Cytokine JT - Cytokine JID - 9005353 RN - 0 (Cytokines) RN - 0 (Inflammation Mediators) SB - IM MH - Aged MH - Aqueous Humor/*immunology MH - Case-Control Studies MH - Cataract/*complications/*immunology MH - Cytokines/*metabolism MH - Female MH - Hepatitis B/*complications MH - Humans MH - Inflammation Mediators/metabolism MH - Male MH - Middle Aged MH - Pain/epidemiology OTO - NOTNLM OT - Cataract OT - Cytokine OT - HBV OT - Inflammation OT - Pain COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2020/07/10 06:00 MHDA- 2021/11/17 06:00 CRDT- 2020/07/10 06:00 PHST- 2019/12/02 00:00 [received] PHST- 2020/06/21 00:00 [revised] PHST- 2020/06/29 00:00 [accepted] PHST- 2020/07/10 06:00 [pubmed] PHST- 2021/11/17 06:00 [medline] PHST- 2020/07/10 06:00 [entrez] AID - S1043-4666(20)30205-2 [pii] AID - 10.1016/j.cyto.2020.155189 [doi] PST - ppublish SO - Cytokine. 2020 Oct;134:155189. doi: 10.1016/j.cyto.2020.155189. Epub 2020 Jul 6.