PMID- 32647124
OWN - NLM
STAT- MEDLINE
DCOM- 20201231
LR  - 20210709
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jul 9
TI  - In vivo characterization of target cells for acute elephant endotheliotropic 
      herpesvirus (EEHV) infection in Asian elephants (Elephas maximus).
PG  - 11402
LID - 10.1038/s41598-020-68413-4 [doi]
LID - 11402
AB  - Elephant endotheliotropic herpesvirus-hemorrhagic disease (EEHV-HD) is a 
      dangerous viral infectious disease in young Asian elephants. Despite hypotheses 
      underlying pathogenesis of the disease, it is unclear which cell types the virus 
      targets during acute or persistent infections. This study investigated the 
      tissues and target cells permissive for EEHV infection and replication in vivo. 
      Rabbit polyclonal antibodies against the non-structural proteins of EEHV, DNA 
      polymerase (EEHV DNAPol), were generated and validated. These were used to 
      examine EEHV infection and replication in various tissues of acute EEHV-HD cases 
      and compared to an EEHV-negative control. The results indicated that viral 
      antigens were distributed throughout the epithelia of the alimentary tract and 
      salivary glands, endothelia and smooth muscle cells, and monocytic lineage cells 
      of the EEHV-infected elephants. Moreover, EEHV DNAPol proteins were also found in 
      the bone marrow cells of the EEHV1A-HD and EEHV1A/4-HD cases. This study 
      demonstrated for the first time the target cells that favor in vivo EEHV 
      replication during acute infection, providing a promising foundation for 
      investigating EEHV propagation in vitro.
FAU - Guntawang, Thunyamas
AU  - Guntawang T
AD  - Department of Veterinary Biosciences and Veterinary Public Health, Faculty of 
      Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
FAU - Sittisak, Tidaratt
AU  - Sittisak T
AD  - Department of Veterinary Biosciences and Veterinary Public Health, Faculty of 
      Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
FAU - Srivorakul, Saralee
AU  - Srivorakul S
AD  - Department of Veterinary Biosciences and Veterinary Public Health, Faculty of 
      Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
AD  - Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Chiang Mai 
      University, Chiang Mai, 50100, Thailand.
FAU - Kochagul, Varankpicha
AU  - Kochagul V
AD  - Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Chiang Mai 
      University, Chiang Mai, 50100, Thailand.
FAU - Photichai, Kornravee
AU  - Photichai K
AD  - Department of Veterinary Biosciences and Veterinary Public Health, Faculty of 
      Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
AD  - Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Chiang Mai 
      University, Chiang Mai, 50100, Thailand.
FAU - Thitaram, Chatchote
AU  - Thitaram C
AD  - Center of Excellence in Elephant and Wildlife Research, Chiang Mai University, 
      Chiang Mai, 50100, Thailand.
AD  - Department of Companion Animals and Wildlife Clinics, Faculty of Veterinary 
      Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
FAU - Sthitmatee, Nattawooti
AU  - Sthitmatee N
AD  - Department of Veterinary Biosciences and Veterinary Public Health, Faculty of 
      Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.
FAU - Hsu, Wei-Li
AU  - Hsu WL
AD  - Graduate Institute of Microbiology and Public Health, College of Veterinary 
      Medicine, National Chung Hsing University, Taichung, 402, Taiwan.
FAU - Pringproa, Kidsadagon
AU  - Pringproa K
AD  - Department of Veterinary Biosciences and Veterinary Public Health, Faculty of 
      Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand. 
      kidsadagon.p@cmu.ac.th.
AD  - Center of Excellence in Elephant and Wildlife Research, Chiang Mai University, 
      Chiang Mai, 50100, Thailand. kidsadagon.p@cmu.ac.th.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200709
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antigens, Viral)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 2.7.7.7 (DNA-Directed DNA Polymerase)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Bone Marrow Cells/virology
MH  - DNA-Directed DNA Polymerase/analysis/chemistry
MH  - Digestive System/virology
MH  - Elephants/*virology
MH  - Endothelial Cells/virology
MH  - Female
MH  - Heart/virology
MH  - Hemorrhagic Disorders/*veterinary/virology
MH  - Herpesviridae/immunology/*isolation & purification/physiology
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Lymph Nodes/virology
MH  - Male
MH  - Models, Molecular
MH  - Monocytes/virology
MH  - Myocytes, Smooth Muscle/virology
MH  - Nervous System/virology
MH  - Organ Specificity
MH  - Protein Conformation
MH  - Recombinant Proteins/chemistry
MH  - Salivary Glands/virology
MH  - Viral Proteins/analysis
MH  - *Viral Tropism
PMC - PMC7347588
COIS- The authors declare no competing interests.
EDAT- 2020/07/11 06:00
MHDA- 2021/01/01 06:00
PMCR- 2020/07/09
CRDT- 2020/07/11 06:00
PHST- 2020/03/20 00:00 [received]
PHST- 2020/06/24 00:00 [accepted]
PHST- 2020/07/11 06:00 [entrez]
PHST- 2020/07/11 06:00 [pubmed]
PHST- 2021/01/01 06:00 [medline]
PHST- 2020/07/09 00:00 [pmc-release]
AID - 10.1038/s41598-020-68413-4 [pii]
AID - 68413 [pii]
AID - 10.1038/s41598-020-68413-4 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jul 9;10(1):11402. doi: 10.1038/s41598-020-68413-4.