PMID- 32648668
OWN - NLM
STAT- MEDLINE
DCOM- 20200924
LR  - 20200924
IS  - 2366-7478 (Print)
IS  - 2366-7478 (Linking)
VI  - 3
IP  - 7
DP  - 2019 Jul
TI  - A Microfluidic Human Model of Blood-Brain Barrier Employing Primary Human 
      Astrocytes.
PG  - e1800335
LID - 10.1002/adbi.201800335 [doi]
AB  - The neurovascular unit (NVU) is the most important biological barrier between 
      vascular districts and central nervous system (CNS) parenchyma, which maintains 
      brain homeostasis, protects the CNS from pathogens penetration, and mediates 
      neuroimmune communication. T lymphocytes migration across the blood-brain barrier 
      is heavily affected in different brain diseases, representing a major target for 
      novel drug development. In vitro models of NVU could represent a primary tool to 
      investigate the molecular events occurring at this interface. To move toward the 
      establishment of personalized therapies, a patient-related NVU-model is set, 
      incorporating human primary astrocytes integrated into a microfluidic platform. 
      The model is morphologically and functionally characterized, proving to be an 
      advantageous tool to investigate human T lymphocytes transmigration and thus the 
      efficacy of potential novel drugs affecting this process.
CI  - (c) 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Lauranzano, Eliana
AU  - Lauranzano E
AUID- ORCID: 0000-0002-9297-3242
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
FAU - Campo, Elena
AU  - Campo E
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
FAU - Rasile, Marco
AU  - Rasile M
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
AD  - Department of Biomedical Science, Laboratory of Pharmacology and Brain Pathology, 
      Humanitas University, Via Rita Levi Montalcini 4, 20090, Pieve Emanuele, MI, 
      Italy.
FAU - Molteni, Raffaella
AU  - Molteni R
AD  - Division of Immunology, Transplantation and Infectious Diseases, Leukocyte 
      Biology Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, 
      Italy.
FAU - Pizzocri, Marco
AU  - Pizzocri M
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
FAU - Passoni, Lorena
AU  - Passoni L
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
FAU - Bello, Lorenzo
AU  - Bello L
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
AD  - Department of Oncology and Hematology, University of Milan, Via Festa del Perdono 
      7, 20122, Milan, Italy.
FAU - Pozzi, Davide
AU  - Pozzi D
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
AD  - Department of Biomedical Science, Laboratory of Pharmacology and Brain Pathology, 
      Humanitas University, Via Rita Levi Montalcini 4, 20090, Pieve Emanuele, MI, 
      Italy.
FAU - Pardi, Ruggero
AU  - Pardi R
AD  - Division of Immunology, Transplantation and Infectious Diseases, Leukocyte 
      Biology Unit, San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, 
      Italy.
AD  - School of Medicine, Vita-Salute San Raffaele University, Via Olgettina 58, 20132, 
      Milan, Italy.
FAU - Matteoli, Michela
AU  - Matteoli M
AUID- ORCID: 0000-0002-3569-7843
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
AD  - Department of Biomedical Science, Laboratory of Pharmacology and Brain Pathology, 
      Humanitas University, Via Rita Levi Montalcini 4, 20090, Pieve Emanuele, MI, 
      Italy.
FAU - Ruiz-Moreno, Ana
AU  - Ruiz-Moreno A
AD  - Humanitas Clinical and Research Center-IRCCS, via Manzoni 56, 20089, Rozzano, MI, 
      Italy.
LA  - eng
PT  - Journal Article
DEP - 20190508
PL  - Germany
TA  - Adv Biosyst
JT  - Advanced biosystems
JID - 101711718
SB  - IM
MH  - Astrocytes/cytology/*metabolism
MH  - Blood-Brain Barrier/cytology/*metabolism
MH  - Humans
MH  - *Microfluidic Analytical Techniques
MH  - *Models, Cardiovascular
MH  - *Models, Neurological
MH  - Primary Cell Culture
OTO - NOTNLM
OT  - T cells
OT  - astrocytes
OT  - blood-brain barrier
OT  - cytokines
OT  - microfluidics
EDAT- 2020/07/11 06:00
MHDA- 2020/09/25 06:00
CRDT- 2020/07/11 06:00
PHST- 2018/12/27 00:00 [received]
PHST- 2019/03/20 00:00 [revised]
PHST- 2020/07/11 06:00 [entrez]
PHST- 2020/07/11 06:00 [pubmed]
PHST- 2020/09/25 06:00 [medline]
AID - 10.1002/adbi.201800335 [doi]
PST - ppublish
SO  - Adv Biosyst. 2019 Jul;3(7):e1800335. doi: 10.1002/adbi.201800335. Epub 2019 May 
      8.