PMID- 32648683
OWN - NLM
STAT- MEDLINE
DCOM- 20201001
LR  - 20231110
IS  - 2366-7478 (Print)
IS  - 2366-7478 (Electronic)
IS  - 2366-7478 (Linking)
VI  - 3
IP  - 12
DP  - 2019 Dec
TI  - Decorating 3D Printed Scaffolds with Electrospun Nanofiber Segments for Tissue 
      Engineering.
PG  - e1900137
LID - 10.1002/adbi.201900137 [doi]
AB  - Repairing large tissue defects often represents a great challenge in clinics due 
      to issues regarding lack of donors, mismatched sizes, irregular shapes, and 
      immune rejection. 3D printed scaffolds are attractive for growing cells and 
      producing tissue constructs because of the intricate control over pore size, 
      porosity, and geometric shape, but the lack of biomimetic surface nanotopography 
      and limited biomolecule presenting capacity render them less efficacious in 
      regulating cell responses. Herein, a facile method for coating 3D printed 
      scaffolds with electrospun nanofiber segments is reported. The surface morphology 
      of modified 3D scaffolds changes dramatically, displaying a biomimetic 
      nanofibrous structure, while the bulk mechanical property, pore size, and 
      porosity are not significantly compromised. The short nanofibers-decorated 3D 
      printed scaffolds significantly promote adhesion and proliferation of 
      pre-osteoblasts and bone marrow mesenchymal stem cells (BMSCs). Further 
      immobilization of bone morphogenetic protein-2 mimicking peptides to nanofiber 
      segments-decorated 3D printed scaffolds show enhanced mRNA expressions of 
      osteogenic markers Runx2, Alp, OCN, and BSP in BMSCs, indicating the enhancement 
      of BMSCs osteogenic differentiation. Together, the combination of 3D printing and 
      electrospinning is a promising approach to greatly expand the functions of 3D 
      printed scaffolds and enhance the efficacy of 3D printed scaffolds for tissue 
      engineering.
CI  - (c) 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Li, Ruiquan
AU  - Li R
AD  - Department of Surgery-Transplant and Holland Regenerative Medicine Program, 
      University of Nebraska Medical Center, Omaha, NE, 68130, USA.
FAU - McCarthy, Alec
AU  - McCarthy A
AD  - Department of Surgery-Transplant and Holland Regenerative Medicine Program, 
      University of Nebraska Medical Center, Omaha, NE, 68130, USA.
FAU - Zhang, Yu Shrike
AU  - Zhang YS
AD  - Division of Engineering in Medicine, Department of Medicine, Brigham and Women's 
      Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.
FAU - Xie, Jingwei
AU  - Xie J
AUID- ORCID: 0000-0002-8126-1397
AD  - Department of Surgery-Transplant and Holland Regenerative Medicine Program, 
      University of Nebraska Medical Center, Omaha, NE, 68130, USA.
LA  - eng
GR  - R21 EB026175/EB/NIBIB NIH HHS/United States
GR  - 1R21 DE027516/DE/NIDCR NIH HHS/United States
GR  - R21 DE027516/DE/NIDCR NIH HHS/United States
GR  - R21 EB025270/EB/NIBIB NIH HHS/United States
GR  - P20 GM103480/GM/NIGMS NIH HHS/United States
GR  - R01 EB028143/EB/NIBIB NIH HHS/United States
GR  - 2P20 GM103480/GM/NIGMS NIH HHS/United States
GR  - R00 CA201603/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20191104
PL  - Germany
TA  - Adv Biosyst
JT  - Advanced biosystems
JID - 101711718
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Electrochemical Techniques/methods
MH  - Mesenchymal Stem Cells/cytology
MH  - Mice
MH  - Nanofibers/*chemistry
MH  - Osteoblasts/cytology
MH  - Osteogenesis
MH  - *Printing, Three-Dimensional
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
PMC - PMC7735424
MID - NIHMS1651958
OTO - NOTNLM
OT  - 3D printed scaffolds
OT  - cellular responses
OT  - electrospinning
OT  - nanofiber segments
OT  - surface coating
COIS- Conflict of Interest The authors declare no financial interests.
EDAT- 2020/07/11 06:00
MHDA- 2020/10/02 06:00
PMCR- 2020/12/14
CRDT- 2020/07/11 06:00
PHST- 2019/06/21 00:00 [received]
PHST- 2019/10/11 00:00 [revised]
PHST- 2020/07/11 06:00 [entrez]
PHST- 2020/07/11 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2020/12/14 00:00 [pmc-release]
AID - 10.1002/adbi.201900137 [doi]
PST - ppublish
SO  - Adv Biosyst. 2019 Dec;3(12):e1900137. doi: 10.1002/adbi.201900137. Epub 2019 Nov 
      4.