PMID- 32649900
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 15
IP  - 2
DP  - 2020 Aug 11
TI  - A Novel GATA2 Protein Reporter Mouse Reveals Hematopoietic Progenitor Cell Types.
PG  - 326-339
LID - S2213-6711(20)30229-0 [pii]
LID - 10.1016/j.stemcr.2020.06.008 [doi]
AB  - The transcription factor (TF) GATA2 plays a key role in organ development and 
      cell fate control in the central nervous, urogenital, respiratory, and 
      reproductive systems, and in primitive and definitive hematopoiesis. Here, we 
      generate a knockin protein reporter mouse line expressing a GATA2VENUS fusion 
      from the endogenous Gata2 genomic locus, with correct expression and localization 
      of GATA2VENUS in different organs. GATA2VENUS expression is heterogeneous in 
      different hematopoietic stem and progenitor cell populations (HSPCs), identifies 
      functionally distinct subsets, and suggests a novel monocyte and mast cell 
      lineage bifurcation point. GATA2 levels further correlate with proliferation and 
      lineage outcome of hematopoietic progenitors. The GATA2VENUS mouse line improves 
      the identification of specific live cell types during embryonic and adult 
      development and will be crucial for analyzing GATA2 protein dynamics in TF 
      networks.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Ahmed, Nouraiz
AU  - Ahmed N
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Kunz, Leo
AU  - Kunz L
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Hoppe, Philipp S
AU  - Hoppe PS
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Loeffler, Dirk
AU  - Loeffler D
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Etzrodt, Martin
AU  - Etzrodt M
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Ortega, German Camargo
AU  - Ortega GC
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Hilsenbeck, Oliver
AU  - Hilsenbeck O
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland.
FAU - Anastassiadis, Konstantinos
AU  - Anastassiadis K
AD  - Biotechnology Center, Technische Universitat Dresden, Tatzberg 47-51, 01307 
      Dresden, Germany.
FAU - Schroeder, Timm
AU  - Schroeder T
AD  - Department of Biosystems Science & Engineering, ETH Zurich, Mattenstrasse 26, 
      4058 Basel, Switzerland. Electronic address: timm.schroeder@bsse.ethz.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200709
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (GATA2 Transcription Factor)
RN  - 0 (Gata2 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Aging/genetics
MH  - Animals
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Embryo, Mammalian/metabolism
MH  - GATA2 Transcription Factor/genetics/*metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Gene Regulatory Networks
MH  - *Genes, Reporter
MH  - Hematopoiesis
MH  - Hematopoietic Stem Cells/*metabolism
MH  - Mast Cells/cytology
MH  - Mice
MH  - Models, Biological
MH  - Monocytes/cytology
MH  - Neutrophils/cytology
MH  - Organ Specificity
MH  - Transcription Factors/metabolism
PMC - PMC7419669
OTO - NOTNLM
OT  - GATA2
OT  - cell fate
OT  - development
OT  - fluorescent protein
OT  - hematopoietic stem and progenitor cell
OT  - monocyte and mast cell lineage
OT  - transcription factor networks
OT  - transgenic mouse
EDAT- 2020/07/11 06:00
MHDA- 2021/06/03 06:00
PMCR- 2020/07/09
CRDT- 2020/07/11 06:00
PHST- 2020/01/13 00:00 [received]
PHST- 2020/06/08 00:00 [revised]
PHST- 2020/06/09 00:00 [accepted]
PHST- 2020/07/11 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/07/11 06:00 [entrez]
PHST- 2020/07/09 00:00 [pmc-release]
AID - S2213-6711(20)30229-0 [pii]
AID - 10.1016/j.stemcr.2020.06.008 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2020 Aug 11;15(2):326-339. doi: 10.1016/j.stemcr.2020.06.008. 
      Epub 2020 Jul 9.