PMID- 32651971
OWN - NLM
STAT- MEDLINE
DCOM- 20211014
LR  - 20211014
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 21
IP  - 1
DP  - 2021 Feb 1
TI  - Dose-dependent effects of adalimumab in neonatal rats with 
      hypoxia/reoxygenation-induced intestinal damage.
PG  - 33-38
LID - 10.17305/bjbms.2020.4823 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has an important role in 
      hypoxia/reoxygenation (H/R)-induced intestinal damage. It was shown that blocking 
      TNF-alpha with infliximab has beneficial effects on experimental necrotizing 
      enterocolitis and hypoxic intestinal injury. However, there is no data about the 
      effect of adalimumab on H/R-induced intestinal damage. Therefore, we aimed to 
      determine potential dose-dependent benefits of adalimumab in such damage in 
      neonatal rats. Wistar albino rat pups were assigned to one of the four groups: 
      control group, hypoxia group, low-dose adalimumab (5 mg/kg/day) treated group 
      (LDAT), and high-dose adalimumab (50 mg/kg/day) treated group (HDAT). On the 
      fourth day of the experiment, all rats except for the control group were exposed 
      to H/R followed by euthanasia. Malondialdehyde (MDA), myeloperoxidase (MPO), 
      TNF-alpha, total antioxidant capacity (TAC), and total oxidant capacity (TOC) were 
      measured in intestinal tissue. TAC and TOC values were used to calculate the 
      oxidative stress index (OSI). Histopathological injury scores (HIS) were also 
      evaluated in the tissue samples. MDA levels were significantly lower in the LDAT 
      and HDAT groups (p < 0.001). TNF-alpha levels were significantly lower in the LDAT 
      group (p < 0.001). OSI was significantly higher in the H/R group than in the 
      control and LDAT groups (p < 0.001). Mean HIS values in the LDAT group were 
      significantly lower than those in the H/R and HDAT groups (p < 0.001). This 
      experimental study showed that low-dose adalimumab appears to have a beneficial 
      effect on intestinal injury induced with H/R in neonatal rats.
FAU - Kocamaz, Halil
AU  - Kocamaz H
AD  - Department of Pediatric Gastroenterology, Faculty of Medicine, Pamukkale 
      University, Denizli, Turkey.
FAU - Ozdemir, Ozmert Ma
AU  - Ozdemir OM
AD  - Division of Neonatology, Department of Pediatrics, Faculty of Medicine, Pamukkale 
      University, Denizli, Turkey.
FAU - Turk, Nilay Sen
AU  - Turk NS
AD  - Department of Pathology, Faculty of Medicine, Pamukkale University, Denizli, 
      Turkey.
FAU - Enli, Yasar
AU  - Enli Y
AD  - Department of Biochemistry, Faculty of Medicine, Pamukkale University, Denizli, 
      Turkey.
FAU - Sahin, Barbaros
AU  - Sahin B
AD  - Experimental Animal Study Laboratory, Pamukkale University, Denizli, Turkey.
FAU - Ergin, Hacer
AU  - Ergin H
AD  - Division of Neonatology, Department of Pediatrics, Faculty of Medicine, Pamukkale 
      University, Denizli, Turkey.
LA  - eng
PT  - Journal Article
DEP - 20210201
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - 0 (Antioxidants)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - FYS6T7F842 (Adalimumab)
SB  - IM
MH  - Adalimumab/administration & dosage/*pharmacology
MH  - Animals
MH  - Animals, Newborn
MH  - Antioxidants/metabolism
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Enterocolitis, Necrotizing/*drug therapy/metabolism/pathology
MH  - Hypoxia/*pathology
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress
MH  - Peroxidase/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/*drug therapy/metabolism/*pathology
PMC - PMC7861631
COIS- Conflict of interest statement: The authors declare no conflict of interests
EDAT- 2020/07/12 06:00
MHDA- 2021/10/15 06:00
PMCR- 2021/02/01
CRDT- 2020/07/12 06:00
PHST- 2020/05/08 00:00 [received]
PHST- 2020/07/02 00:00 [accepted]
PHST- 2020/07/12 06:00 [pubmed]
PHST- 2021/10/15 06:00 [medline]
PHST- 2020/07/12 06:00 [entrez]
PHST- 2021/02/01 00:00 [pmc-release]
AID - BJBMS-21-33 [pii]
AID - 10.17305/bjbms.2020.4823 [doi]
PST - epublish
SO  - Bosn J Basic Med Sci. 2021 Feb 1;21(1):33-38. doi: 10.17305/bjbms.2020.4823.