PMID- 32652105
OWN - NLM
STAT- MEDLINE
DCOM- 20200826
LR  - 20211204
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 757
DP  - 2020 Oct 5
TI  - Overexpression of the Tuberous sclerosis complex 2 (TSC2) gene inhibits goat 
      myoblasts proliferation and differentiation in understanding the underlying 
      mechanism of muscle development.
PG  - 144943
LID - S0378-1119(20)30612-0 [pii]
LID - 10.1016/j.gene.2020.144943 [doi]
AB  - The growth of animal skeletal muscle is mainly determined by the synthesis 
      processes of total proteins in skeletal muscle cells, which has a significant 
      impact on the postnatal growth of young animals. An increasing number of studies 
      are focusing on the functions of Tuberous sclerosis complex 2 (TSC2) during the 
      process of cell protein synthesis and growth. However, it is still unclear the 
      effect of whether and how TSC2 on goat myoblasts proliferation and 
      differentiation. Here, we found that TSC2 gene has opposite expression patterns 
      in proliferation and differentiation of myoblasts. An expression vector 
      containing goat TSC2 cDNA sequences linked with pcDNA3.1 plasmid was constructed. 
      Myoblasts proliferation activity was significantly inhibited and cell cycle 
      transition slowed down after the transfection of pcDNA3.1-TSC2 plasmid into goat 
      primary myoblasts by EdU staining, CCK-8 and flow cytometry. Mechanically, we 
      further confirmed that the overexpression TSC2 was able to down-regulate the mRNA 
      and protein expression of mechanistic target of rapamycin (mTOR), p70 ribosomal 
      S6 kinase 1 (p70S6K) and some cell cycle related genes. In addition, the 
      expression of myogenic genes and myotube formation were attenuated. Collectively, 
      all our results of the experiment demonstrate that TSC2 could regulate myoblasts 
      cells proliferation and differentiation via the activation of the mTOR/p70S6K 
      signaling pathway.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Lyu, Ming
AU  - Lyu M
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Shalitana, Alai
AU  - Shalitana A
AD  - Department of Xinjiang Institute for Cancer Research, Xinjiang Cancer Hospital of 
      Xinjiang Medical University, Urumqi 830011, China.
FAU - Luo, Jun
AU  - Luo J
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - He, Huanshan
AU  - He H
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Sun, Shuang
AU  - Sun S
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Wang, Ping
AU  - Wang P
AD  - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, 
      College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China. Electronic address: wangping@nwafu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200709
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Tuberous Sclerosis Complex 2 Protein)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Goats
MH  - *Muscle Development
MH  - Myoblasts/cytology/*metabolism/physiology
MH  - Ribosomal Protein S6 Kinases, 70-kDa/genetics/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Tuberous Sclerosis Complex 2 Protein/*genetics/metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Differentiation
OT  - Goat myoblasts
OT  - MTOR/p70S6K
OT  - Proliferation
OT  - TSC2
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/07/12 06:00
MHDA- 2020/08/28 06:00
CRDT- 2020/07/12 06:00
PHST- 2019/10/27 00:00 [received]
PHST- 2020/06/26 00:00 [revised]
PHST- 2020/07/01 00:00 [accepted]
PHST- 2020/07/12 06:00 [pubmed]
PHST- 2020/08/28 06:00 [medline]
PHST- 2020/07/12 06:00 [entrez]
AID - S0378-1119(20)30612-0 [pii]
AID - 10.1016/j.gene.2020.144943 [doi]
PST - ppublish
SO  - Gene. 2020 Oct 5;757:144943. doi: 10.1016/j.gene.2020.144943. Epub 2020 Jul 9.