PMID- 32653617
OWN - NLM
STAT- MEDLINE
DCOM- 20210804
LR  - 20210804
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 170
DP  - 2021 Sep
TI  - Susceptibility to extinction and reinstatement of ethanol-induced conditioned 
      place preference is related to differences in astrocyte cystine-glutamate 
      antiporter content.
PG  - 245-254
LID - S0168-0102(20)30402-8 [pii]
LID - 10.1016/j.neures.2020.07.002 [doi]
AB  - Individual susceptibility to alcohol effects plays an important role in the 
      development of alcohol addiction and studies have shown that glutamate release is 
      altered after chronic ethanol consumption. The cystine-glutamate antiporter (xCT) 
      is a protein that regulates glutamate release. However, little is known about the 
      relationship between xCT levels and this individual susceptibility. Thus, this 
      study aimed to evaluate the relationship between the extinction and 
      stress-induced reinstatement of ethanol conditioned place preference (CPP) and 
      xCT levels in the medial prefrontal cortex (mPFC), nucleus accumbens (NAcc) and 
      amygdala (Amy). Male Swiss mice were submitted to a CPP procedure followed by an 
      extinction protocol and then identified as those which extinguished the CPP and 
      those that did not. In another cohort, mice that extinguished the CPP were 
      submitted to a protocol of stress-induced reinstatement. Immediately after the 
      tests, brains were removed for xCT quantification. The xCT levels were 
      significantly lower in the mPFC and NAcc of mice that did not extinguish CPP. 
      Moreover, mice that were susceptible to stress-induced reinstatement of CPP had 
      lower levels of xCT in the NAcc. Our results suggest that individual 
      susceptibility to the extinction and reinstatement of ethanol CPP is related to 
      alterations in xCT levels.
CI  - Copyright (c) 2020 Elsevier B.V. and Japan Neuroscience Society. All rights 
      reserved.
FAU - Amaral, Vanessa Cristiane Santana
AU  - Amaral VCS
AD  - Laboratory of Pharmacology and Toxicology of Natural and Synthetic Products, 
      State University of Goias, Exact and Technological Sciences Campus, Anapolis, GO, 
      Brazil; Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, 
      Laboratory of Pharmacology, Araraquara, SP, Brazil; Joint Graduate Program in 
      Physiological Sciences (PIPGCF), UFSCar/UNESP, Sao Carlos/Araraquara, SP, Brazil.
FAU - Morais-Silva, Gessynger
AU  - Morais-Silva G
AD  - Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Laboratory 
      of Pharmacology, Araraquara, SP, Brazil; Joint Graduate Program in Physiological 
      Sciences (PIPGCF), UFSCar/UNESP, Sao Carlos/Araraquara, SP, Brazil.
FAU - Laverde, Celina F
AU  - Laverde CF
AD  - Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Laboratory 
      of Pharmacology, Araraquara, SP, Brazil; Joint Graduate Program in Physiological 
      Sciences (PIPGCF), UFSCar/UNESP, Sao Carlos/Araraquara, SP, Brazil.
FAU - Marin, Marcelo T
AU  - Marin MT
AD  - Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Laboratory 
      of Pharmacology, Araraquara, SP, Brazil; Joint Graduate Program in Physiological 
      Sciences (PIPGCF), UFSCar/UNESP, Sao Carlos/Araraquara, SP, Brazil. Electronic 
      address: marcelo.marin@unesp.br.
LA  - eng
PT  - Journal Article
DEP - 20200709
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Antiporters)
RN  - 3K9958V90M (Ethanol)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 48TCX9A1VT (Cystine)
SB  - IM
MH  - Animals
MH  - Antiporters
MH  - Astrocytes
MH  - Cystine
MH  - *Drug-Seeking Behavior
MH  - *Ethanol
MH  - Glutamic Acid
MH  - Male
MH  - Mice
OTO - NOTNLM
OT  - Conditioned place preference
OT  - Cystine-glutamate antiporter
OT  - Ethanol
OT  - Medial prefrontal cortex
OT  - Nucleus accumbens
OT  - Reinstatement
EDAT- 2020/07/13 06:00
MHDA- 2021/08/05 06:00
CRDT- 2020/07/13 06:00
PHST- 2020/04/02 00:00 [received]
PHST- 2020/06/22 00:00 [revised]
PHST- 2020/07/07 00:00 [accepted]
PHST- 2020/07/13 06:00 [pubmed]
PHST- 2021/08/05 06:00 [medline]
PHST- 2020/07/13 06:00 [entrez]
AID - S0168-0102(20)30402-8 [pii]
AID - 10.1016/j.neures.2020.07.002 [doi]
PST - ppublish
SO  - Neurosci Res. 2021 Sep;170:245-254. doi: 10.1016/j.neures.2020.07.002. Epub 2020 
      Jul 9.