PMID- 32654099
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20210524
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 199
IP  - 4
DP  - 2021 Apr
TI  - Ameliorative Effect of Ananas comosus on Cobalt Chloride-Induced Hypoxia in Caco2 
      cells via HIF-1alpha, GLUT 1, VEGF, ANG and FGF.
PG  - 1345-1355
LID - 10.1007/s12011-020-02278-6 [doi]
AB  - In the present study, protective effects of Ananas comosus i.e., pineapple pulp 
      (PA) against cobalt chloride (CoCl(2))‑induced hypoxia in Caco-2 cells were 
      evaluated. PA reduces levels of lipid peroxidation, reactive oxygen and nitrogen 
      species. It was proved to be cytoprotective and increased anti-oxidant activity 
      against CoCl(2)-induced hypoxia. The inference drawn from this experiment was 
      CoCl(2)-induced hypoxia that regulates hypoxia-inducible factor-1 (HIF-1), a 
      transcription factor. It was also confirmed that PA pre-treatment inhibited the 
      expression of HIF-1alpha, thereby downregulating the hypoxia-associated gene/protein 
      expressions such as GLUT-1, VEGF, ANG and FGF. Finally, supplementation of PA 
      could help in snow-balling the digestive hormones like leptin and CCK in hypoxic 
      conditions. Therefore, this report provides substantial proof of reducing the 
      hypoxia-induced loss of appetite at high-altitude environments. Graphical 
      Abstract.
FAU - Basavaraju, Anusha Maliyur
AU  - Basavaraju AM
AD  - Food Quality Assurance, Defence Food Research Laboratory, Defence Research and 
      Development Organisation, Ministry of Defence, Govt. of India, Siddarthanagar, 
      Mysore, 570011, India.
FAU - Shivanna, Naveen
AU  - Shivanna N
AUID- ORCID: 0000-0002-1936-4299
AD  - Food Quality Assurance, Defence Food Research Laboratory, Defence Research and 
      Development Organisation, Ministry of Defence, Govt. of India, Siddarthanagar, 
      Mysore, 570011, India. naveens.dfrl@gmail.com.
FAU - Yadavalli, Chandrasekhar
AU  - Yadavalli C
AD  - Food Quality Assurance, Defence Food Research Laboratory, Defence Research and 
      Development Organisation, Ministry of Defence, Govt. of India, Siddarthanagar, 
      Mysore, 570011, India.
FAU - Garlapati, Phani Kumar
AU  - Garlapati PK
AD  - Food Quality Assurance, Defence Food Research Laboratory, Defence Research and 
      Development Organisation, Ministry of Defence, Govt. of India, Siddarthanagar, 
      Mysore, 570011, India.
FAU - Raghavan, Anilakumar Kandangath
AU  - Raghavan AK
AD  - Food Quality Assurance, Defence Food Research Laboratory, Defence Research and 
      Development Organisation, Ministry of Defence, Govt. of India, Siddarthanagar, 
      Mysore, 570011, India.
LA  - eng
PT  - Journal Article
DEP - 20200711
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Glucose Transporter Type 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 3G0H8C9362 (Cobalt)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - EVS87XF13W (cobaltous chloride)
SB  - IM
MH  - *Ananas/metabolism
MH  - Caco-2 Cells
MH  - *Cell Hypoxia
MH  - *Cobalt/toxicity
MH  - Fibroblast Growth Factors
MH  - Glucose Transporter Type 1
MH  - Humans
MH  - Hypoxia
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - Ananas comosus
OT  - Cobalt chloride
OT  - HIF-1alpha
OT  - High altitude
OT  - Hypoxia
OT  - Leptin
EDAT- 2020/07/13 06:00
MHDA- 2021/05/25 06:00
CRDT- 2020/07/13 06:00
PHST- 2020/05/14 00:00 [received]
PHST- 2020/06/29 00:00 [accepted]
PHST- 2020/07/13 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2020/07/13 06:00 [entrez]
AID - 10.1007/s12011-020-02278-6 [pii]
AID - 10.1007/s12011-020-02278-6 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2021 Apr;199(4):1345-1355. doi: 10.1007/s12011-020-02278-6. 
      Epub 2020 Jul 11.