PMID- 32655588
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231103
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 11
DP  - 2020
TI  - Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, 
      Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization 
      Karyotyping.
PG  - 710
LID - 10.3389/fpls.2020.00710 [doi]
LID - 710
AB  - Fluorescence in situ hybridization karyotypes have been widely used for 
      evolutionary analysis on chromosome organization and genetic/genomic diversity in 
      the wheat alliance (tribe Triticeae of Poaceae). The karyotpic diversity of 
      Aegilops umbellulata, Ae. markgrafii, Ae. comosa subsp. comosa and subsp. 
      subventricosa, and Ae. uniaristata was evaluated by the fluorescence in situ 
      hybridization (FISH) probes oligo-pSc119.2 and pTa71 in combination with 
      (AAC)(5), (ACT)(7), and (CTT)(12), respectively. Abundant intra- and 
      interspecific genetic variation was discovered in Ae. umbellulata, Ae. 
      markgrafii, and Ae. comosa, but not Ae. uniaristata. Chromosome 7 of Ae. 
      umbellulata had more variants (six variants) than the other six U chromosomes 
      (2-3 variants) as revealed by probes oligo-pSc119.2 and (AAC)(5). Intraspecific 
      variation in Ae. markgrafii and Ae. comosa was revealed by oligo-pSc119.2 in 
      combination with (ACT)(7) and (CTT)(12), respectively. At least five variants 
      were found in every chromosome of Ae. markgrafii and Ae. comosa, and up to 18, 
      10, and 15 variants were identified for chromosomes 2 of Ae. markgrafii, 4 of Ae. 
      comosa subsp. comosa, and 6 of Ae. comosa subsp. subventricosa. The six Ae. 
      uniaristata accessions showed identical FISH signal patterns. A large number of 
      intra-specific polymorphic FISH signals were observed between the homologous 
      chromosomes of Ae. markgrafii and Ae. comosa, especially chromosomes 1, 2, 4, and 
      7 of Ae. markgrafii, chromosome 4 of Ae. comosa subsp. comosa, and chromosome 6 
      of Ae. comosa subsp. subventricosa. Twelve Ae. comosa and 24 Ae. markgrafii 
      accessions showed heteromorphism between homologous chromosomes. Additionally, a 
      translocation between the short arms of chromosomes 1 and 7 of Ae. comosa PI 
      551038 was identified. The FISH karyotypes can be used to clearly identify the 
      chromosome variations of each chromosome in these Aegilops species and also 
      provide valuable information for understanding the evolutionary relationships and 
      structural genomic variation among Aegilops species.
CI  - Copyright (c) 2020 Song, Dai, Bao, Zuo, Xiang, Li, Liu and Yan.
FAU - Song, Zhongping
AU  - Song Z
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Wenjiang, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Dai, Shoufen
AU  - Dai S
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Wenjiang, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Bao, Tingyu
AU  - Bao T
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Zuo, Yuanyuan
AU  - Zuo Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Xiang, Qin
AU  - Xiang Q
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Li, Jian
AU  - Li J
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Liu, Gang
AU  - Liu G
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
FAU - Yan, Zehong
AU  - Yan Z
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Wenjiang, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, China.
LA  - eng
PT  - Journal Article
DEP - 20200609
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7325912
OTO - NOTNLM
OT  - Ae. comosa
OT  - Ae. markgrafii
OT  - Ae. umbellulata
OT  - Ae. uniaristata
OT  - chromosomal variation
OT  - diploid Aegilops species
OT  - fluorescence in situ hybridization (FISH)
OT  - karyotypes
EDAT- 2020/07/14 06:00
MHDA- 2020/07/14 06:01
PMCR- 2020/01/01
CRDT- 2020/07/14 06:00
PHST- 2019/12/25 00:00 [received]
PHST- 2020/05/05 00:00 [accepted]
PHST- 2020/07/14 06:00 [entrez]
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2020/07/14 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2020.00710 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 Jun 9;11:710. doi: 10.3389/fpls.2020.00710. eCollection 
      2020.