PMID- 32655811
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 12
IP  - 6
DP  - 2020
TI  - Methacholine induced airway contraction in porcine precision cut lung slices from 
      indoor and outdoor reared pigs.
PG  - 2805-2813
AB  - Repetitive exposure to bioaerosols in swine production facilities (SPF) promotes 
      respiratory dysfunction in workers and animals. An adequate understanding of the 
      impact of the SPF environment on pulmonary physiology is needed. However, there 
      is currently no sufficient ex vivo model to investigate the cause for 
      agriculture-related lung disease. The precision cut lung slices (PCLS) technique 
      represents a practical and useful procedure for ex vivo studies. Our goal was to 
      use the PCLS technique to develop a model of agriculture-related lung diseases 
      using a physiologically relevant animal model, the domesticated pig. Freshly 
      prepared pig lung tissue cores were sectioned into 300 microm slices and viability 
      was measured by lactate dehydrogenase activity and live/dead staining. Airway 
      contractility in response to a methacholine (MCh) dose gradient (10(-7)-10(-4) M) 
      was measured. After the last MCh dose, PCLS were incubated with 1 mM chloroquine 
      to allow airways to relax. Time-lapse images were taken every minute for 35 
      minutes and used to determine airway lumen area changes. Porcine PCLS remained 
      viable and demonstrated metabolic activity for three days. PCLS from indoor and 
      outdoor pigs contracted in response to MCh exposure and relaxed when incubated 
      with chloroquine. Notably, PCLS of indoor pigs showed greater airway constriction 
      in response to 10(-5) M MCh exposure compared to outdoor pig PCLS (P<0.05). These 
      data suggest that exposure to the indoor pig production environment may be 
      associated with hyperresponsiveness in swine airways, and support future studies 
      to investigate lung response to inflammatory substances using the porcine PCLS 
      model.
CI  - AJTR Copyright (c) 2020.
FAU - Barton, Ke'Yona T
AU  - Barton KT
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
FAU - Conklin, Dawn R
AU  - Conklin DR
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
FAU - Ranabhat, Rohit S
AU  - Ranabhat RS
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
AD  - Applied Science and Technology Program, North Carolina Agricultural and Technical 
      State University Greensboro, USA.
FAU - Harper, Marquis
AU  - Harper M
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
FAU - Holmes-Cobb, La'Neesa M
AU  - Holmes-Cobb LM
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
FAU - Martinez Soto, Margarita H
AU  - Martinez Soto MH
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
FAU - Waterman, Jenora T
AU  - Waterman JT
AD  - Department of Animal Sciences, North Carolina Agricultural and Technical State 
      University Greensboro, USA.
LA  - eng
GR  - SC1 HL150742/HL/NHLBI NIH HHS/United States
GR  - UL1 TR001111/TR/NCATS NIH HHS/United States
PT  - Journal Article
DEP - 20200615
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC7344084
OTO - NOTNLM
OT  - Precision cut lung slice
OT  - airway contractility
OT  - methacholine
OT  - pig
OT  - swine production facility
COIS- None.
EDAT- 2020/07/14 06:00
MHDA- 2020/07/14 06:01
PMCR- 2020/06/15
CRDT- 2020/07/14 06:00
PHST- 2020/03/03 00:00 [received]
PHST- 2020/04/20 00:00 [accepted]
PHST- 2020/07/14 06:00 [entrez]
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2020/07/14 06:01 [medline]
PHST- 2020/06/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2020 Jun 15;12(6):2805-2813. eCollection 2020.