PMID- 32655821
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 12
IP  - 6
DP  - 2020
TI  - Long non-coding RNA KRT16P2/miR-1294/EGFR axis regulates laryngeal squamous cell 
      carcinoma cell aggressiveness.
PG  - 2939-2955
AB  - Laryngeal squamous cell carcinoma (LSCC) is one of the most commonly seen head 
      and neck malignancies. Identifying potent markers and/or targets for early 
      diagnosis and individualized therapies for LSCC remains a considerable challenge. 
      The present study analyzed online data and identified lncRNA KRT16P2 as a 
      significantly upregulated long non-coding RNA (lncRNA) in LSCC. KRT16P2 knockdown 
      in LSCC cells inhibited cancer cell proliferation, invasion, and migration. 
      Similar to KRT16P2, EGFR expression was also significantly upregulated in LSCC. 
      KRT16P2 and EGFR were positively correlated in LSCC tissue samples. EGFR 
      knockdown also dramatically inhibited LSCC cell proliferation and aggressiveness 
      (invasion and migration). Through online data and online tools, miR-1294 was 
      predicted to target KRT16P2 and EGFR 3'UTR simultaneously. KRT16P2 inhibited 
      miR-1294 expression, and miR-1294 inhibited EGFR expression through direct 
      binding. miR-1294 overexpression repressed LSCC cell proliferation and 
      aggressiveness. The effects of KRT16P2 silence on the expression of EGFR, LSCC 
      cell proliferation, invasion, and migration, the protein levels of ki-67, PCNA, 
      and cleaved-Caspase 3, as well as the phosphorylation of AKT, were all 
      significantly reversed by miR-1294 inhibition. In conclusion, we demonstrated a 
      lncRNA KRT16P2/miR-1294/EGFR axis that regulates LSCC cell proliferation, 
      invasion, and migration. The clinical application of this axis needs further in 
      vivo and clinical investigation.
CI  - AJTR Copyright (c) 2020.
FAU - Yang, Tao
AU  - Yang T
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
FAU - Li, Shisheng
AU  - Li S
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
FAU - Liu, Jiajia
AU  - Liu J
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
FAU - Yin, Danhui
AU  - Yin D
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
FAU - Yang, Xinming
AU  - Yang X
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
FAU - Tang, Qinglai
AU  - Tang Q
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
FAU - Wang, Shuhui
AU  - Wang S
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital, 
      Central South University Changsha 410011, Hunan, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200615
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC7344088
OTO - NOTNLM
OT  - EGFR
OT  - Laryngeal squamous cell carcinoma (LSCC)
OT  - cell aggressiveness
OT  - long non-coding RNA (lncRNA) KRT16P2
OT  - miR-1294
COIS- None.
EDAT- 2020/07/14 06:00
MHDA- 2020/07/14 06:01
PMCR- 2020/06/15
CRDT- 2020/07/14 06:00
PHST- 2019/11/19 00:00 [received]
PHST- 2020/05/18 00:00 [accepted]
PHST- 2020/07/14 06:00 [entrez]
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2020/07/14 06:01 [medline]
PHST- 2020/06/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2020 Jun 15;12(6):2939-2955. eCollection 2020.