PMID- 32655973
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 20
IP  - 3
DP  - 2020 Jun
TI  - Cathelicidin-related Antimicrobial Peptide Contributes to Host Immune Responses 
      Against Pulmonary Infection with Acinetobacter baumannii in Mice.
PG  - e25
LID - 10.4110/in.2020.20.e25 [doi]
LID - e25
AB  - Acinetobacter baumannii is known for its multidrug antibiotic resistance. New 
      approaches to treating drug-resistant bacterial infections are urgently required. 
      Cathelicidin-related antimicrobial peptide (CRAMP) is a murine antimicrobial 
      peptide that exerts diverse immune functions, including both direct bacterial 
      cell killing and immunomodulatory effects. In this study, we sought to identify 
      the role of CRAMP in the host immune response to multidrug-resistant 
      Acinetobacter baumannii. Wild-type (WT) and CRAMP knockout mice were infected 
      intranasally with the bacteria. CRAMP(-/-) mice exhibited increased bacterial 
      colony-forming units (CFUs) in bronchoalveolar lavage (BAL) fluid after A. 
      baumannii infection compared to WT mice. The loss of CRAMP expression resulted in 
      a significant decrease in the recruitment of immune cells, primarily neutrophils. 
      The levels of IL-6 and CXCL1 were lower, whereas the levels of IL-10 were 
      significantly higher in the BAL fluid of CRAMP(-/-) mice compared to WT mice 1 
      day after infection. In an in vitro assay using thioglycollate-induced peritoneal 
      neutrophils, the ability of bacterial phagocytosis and killing was impaired in 
      CRAMP(-/-) neutrophils compared to the WT cells. CRAMP was also essential for the 
      production of cytokines and chemokines in response to A. baumannii in 
      neutrophils. In addition, the A. baumannii-induced inhibitor of kappaB-alpha degradation 
      and phosphorylation of p38 MAPK were impaired in CRAMP(-/-) neutrophils, whereas 
      ERK and JNK phosphorylation was upregulated. Our results indicate that CRAMP 
      plays an important role in the host defense against pulmonary infection with A. 
      baumannii by promoting the antibacterial activity of neutrophils and regulating 
      the innate immune responses.
CI  - Copyright (c) 2020. The Korean Association of Immunologists.
FAU - Kang, Min-Jung
AU  - Kang MJ
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Jang, Ah-Ra
AU  - Jang AR
AUID- ORCID: 0000-0003-2378-5720
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Park, Ji-Yeon
AU  - Park JY
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Ahn, Jae-Hun
AU  - Ahn JH
AUID- ORCID: 0000-0003-4545-1211
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Lee, Tae-Sung
AU  - Lee TS
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Kim, Dong-Yeon
AU  - Kim DY
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Jung, Do-Hyeon
AU  - Jung DH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Song, Eun-Jung
AU  - Song EJ
AUID- ORCID: 0000-0002-1495-488X
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
FAU - Hong, Jung Joo
AU  - Hong JJ
AUID- ORCID: 0000-0002-9795-6513
AD  - National Primate Research Center, Korea Research Institute of Bioscience and 
      Biotechnology, Chungcheongbuk 28116, Korea.
FAU - Park, Jong-Hwan
AU  - Park JH
AUID- ORCID: 0000-0003-4664-5640
AD  - Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National 
      University, Gwangju, Korea.
LA  - eng
PT  - Journal Article
DEP - 20200520
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC7327154
OTO - NOTNLM
OT  - Acinetobacter baumannii
OT  - CRAMP
OT  - Innate immune response
OT  - Neutrophils
COIS- Conflicts of Interest: The authors declare no potential conflicts of interest.
EDAT- 2020/07/14 06:00
MHDA- 2020/07/14 06:01
PMCR- 2020/06/01
CRDT- 2020/07/14 06:00
PHST- 2020/01/08 00:00 [received]
PHST- 2020/04/27 00:00 [revised]
PHST- 2020/05/06 00:00 [accepted]
PHST- 2020/07/14 06:00 [entrez]
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2020/07/14 06:01 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - 2020200306 [pii]
AID - 10.4110/in.2020.20.e25 [doi]
PST - epublish
SO  - Immune Netw. 2020 May 20;20(3):e25. doi: 10.4110/in.2020.20.e25. eCollection 2020 
      Jun.