PMID- 32655974
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 20
IP  - 3
DP  - 2020 Jun
TI  - Cereblon Deletion Ameliorates Lipopolysaccharide-induced Proinflammatory 
      Cytokines through 5'-Adenosine Monophosphate-Activated Protein Kinase/Heme 
      Oxygenase-1 Activation in ARPE-19 Cells.
PG  - e26
LID - 10.4110/in.2020.20.e26 [doi]
LID - e26
AB  - Cereblon (CRBN), a negative modulator of AMP-activated protein kinase (AMPK), is 
      highly expressed in the retina. We confirmed the expression of CRBN in ARPE-19 
      human retinal cells by Western blotting. We also demonstrated that CRBN 
      knock-down (KD) could effectively downregulate IL-6 and MCP-1 protein and gene 
      expression in LPS-stimulated ARPE-19 cells. Additionally, CRBN KD increased the 
      phosphorylation of AMPK/acetyl-coenzyme A carboxylase (ACC) and the expression of 
      heme oxygenase-1 (HO-1) in ARPE-19 cells. Furthermore, CRBN KD significantly 
      reduced LPS-induced nuclear translocation of NF-kappaB p65 and activation of NF-kappaB 
      promoter activity. However, these processes could be inactivated by compound C 
      (inhibitor of AMPK) and zinc protoporphyrin-1 (ZnPP-1; inhibitor of HO-1). In 
      conclusion, compound C and ZnPP-1 can rescue LPS-induced levels of 
      proinflammatory cytokines (IL-6 and MCP-1) in CRBN KD ARPE-19 cells. Our data 
      demonstrate that CRBN deficiency negatively regulates proinflammatory cytokines 
      via the activation of AMPK/HO-1 in the retina.
CI  - Copyright (c) 2020. The Korean Association of Immunologists.
FAU - Kim, Yun Kyu
AU  - Kim YK
AUID- ORCID: 0000-0002-8403-945X
AD  - Department of Convergence Medicine, Asan Institute for Life Sciences, University 
      of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
FAU - Chae, Soo Chul
AU  - Chae SC
AD  - Department of Integrative Medical Sciences, Nambu University, Gwangju 62271, 
      Korea.
AD  - Department of Life Science, Hanyang University, Seoul 04763, Korea.
FAU - Yang, Hun Ji
AU  - Yang HJ
AD  - Department of Convergence Medicine, Asan Institute for Life Sciences, University 
      of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
FAU - An, Da Eun
AU  - An DE
AD  - Department of Convergence Medicine, Asan Institute for Life Sciences, University 
      of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
FAU - Lee, Sion
AU  - Lee S
AD  - Department of Convergence Medicine, Asan Institute for Life Sciences, University 
      of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
FAU - Yeo, Myeong Gu
AU  - Yeo MG
AD  - Department of Integrative Medical Sciences, Nambu University, Gwangju 62271, 
      Korea.
FAU - Lee, Kyung Jin
AU  - Lee KJ
AUID- ORCID: 0000-0002-5282-5683
AD  - Department of Convergence Medicine, Asan Institute for Life Sciences, University 
      of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea.
AD  - Department of Life Science, Hanyang University, Seoul 04763, Korea.
LA  - eng
PT  - Journal Article
DEP - 20200625
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC7327155
OTO - NOTNLM
OT  - AMP-activated protein kinase
OT  - Cereblon
OT  - Cytokines
OT  - Heme oxygenase-1
OT  - Inflammation
OT  - Retina
COIS- Conflicts of Interest: The authors declare no potential conflicts of interest.
EDAT- 2020/07/14 06:00
MHDA- 2020/07/14 06:01
PMCR- 2020/06/01
CRDT- 2020/07/14 06:00
PHST- 2020/03/26 00:00 [received]
PHST- 2020/06/19 00:00 [revised]
PHST- 2020/06/20 00:00 [accepted]
PHST- 2020/07/14 06:00 [entrez]
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2020/07/14 06:01 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - 2020200307 [pii]
AID - 10.4110/in.2020.20.e26 [doi]
PST - epublish
SO  - Immune Netw. 2020 Jun 25;20(3):e26. doi: 10.4110/in.2020.20.e26. eCollection 2020 
      Jun.