PMID- 32656428
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2470-1343 (Electronic)
IS  - 2470-1343 (Linking)
VI  - 5
IP  - 26
DP  - 2020 Jul 7
TI  - Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on 
      Neuroinflammation of Spinal Cord Injury.
PG  - 16064-16075
LID - 10.1021/acsomega.0c01379 [doi]
AB  - Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases 
      intermediary filaments and neurodegeneration. Previous studies have shown that a 
      basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) 
      contribute to a protective effect on injured neuronal cells, but the mechanism of 
      SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel 
      injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies 
      of bFGF and DPSCs, proved an effective control over inflammation. The in vivo 
      application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated 
      the nerve regeneration through microtubule stabilization and tissue vasculature. 
      Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited 
      microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs 
      prevented microglia/macrophage activation and reduced proinflammatory cytokine 
      release. In this paper, we discovered that bFGF and DPSCs worked together to 
      attenuate tissue inflammation of the injured spinal cord, resulting in a superior 
      nerve repair. Our results indicated that a thermosensitive hydrogel delivering 
      bFGF and DPSCs could serve as a promising treatment option for spinal cord 
      injuries.
CI  - Copyright (c) 2020 American Chemical Society.
FAU - Albashari, Abdullkhaleg
AU  - Albashari A
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
FAU - He, Yan
AU  - He Y
AD  - Laboratory for Regenerative Medicine, Tianyou Hospital, Wuhan University of 
      Science and Technology, Wuhan, Hubei 430064, China.
AD  - School of Dentistry, The University of Queensland, 288 Herston Road, Brisbane, 
      QLD 4006, Australia.
FAU - Zhang, Yanni
AU  - Zhang Y
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
FAU - Ali, Jihea
AU  - Ali J
AD  - College of Life and Environmental Science, Wenzhou University, Wenzhou, Zhejiang 
      325035, China.
FAU - Lin, Feiou
AU  - Lin F
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
FAU - Zheng, Zengming
AU  - Zheng Z
AD  - The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Zhang, Keke
AU  - Zhang K
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
FAU - Cao, Yanfan
AU  - Cao Y
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
FAU - Xu, Chun
AU  - Xu C
AD  - School of Dentistry, The University of Queensland, 288 Herston Road, Brisbane, 
      QLD 4006, Australia.
FAU - Luo, Lihua
AU  - Luo L
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
FAU - Wang, Jianming
AU  - Wang J
AD  - Laboratory for Regenerative Medicine, Tianyou Hospital, Wuhan University of 
      Science and Technology, Wuhan, Hubei 430064, China.
FAU - Ye, Qingsong
AU  - Ye Q
AD  - School and Hospital of Stomatology, Wenzhou Medical University, Wenzhou, Zhejiang 
      325035, China.
AD  - Centre of Regenerative Medicine, Renmin Hospital of Wuhan University, Wuhan 
      University, Wuhan, Hubei 430060, China.
AD  - School of Dentistry, The University of Queensland, 288 Herston Road, Brisbane, 
      QLD 4006, Australia.
LA  - eng
PT  - Journal Article
DEP - 20200625
PL  - United States
TA  - ACS Omega
JT  - ACS omega
JID - 101691658
PMC - PMC7346236
COIS- The authors declare no competing financial interest.
EDAT- 2020/07/14 06:00
MHDA- 2020/07/14 06:01
PMCR- 2020/06/25
CRDT- 2020/07/14 06:00
PHST- 2020/04/06 00:00 [received]
PHST- 2020/06/10 00:00 [accepted]
PHST- 2020/07/14 06:00 [entrez]
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2020/07/14 06:01 [medline]
PHST- 2020/06/25 00:00 [pmc-release]
AID - 10.1021/acsomega.0c01379 [doi]
PST - epublish
SO  - ACS Omega. 2020 Jun 25;5(26):16064-16075. doi: 10.1021/acsomega.0c01379. 
      eCollection 2020 Jul 7.