PMID- 32658559
OWN - NLM
STAT- MEDLINE
DCOM- 20210521
LR  - 20210521
IS  - 1362-3095 (Electronic)
IS  - 0955-3002 (Linking)
VI  - 96
IP  - 9
DP  - 2020 Sep
TI  - Cellular kinetics of hematopoietic cells with Sfpi1 deletion are present at 
      different frequencies in bone-marrow and spleen in X-irradiated mice.
PG  - 1119-1124
LID - 10.1080/09553002.2020.1793018 [doi]
AB  - PURPOSE: Several past studies using a mouse model of radiation-induced AML (rAML) 
      have shown that hemizygous deletion of the Sfpi1 gene (HDSG) is an initiating 
      event for the development of rAML. In this study, we examined the difference in 
      frequency of HDSG in hematopoietic stem cells (HSCs) Rich hematopoietic Cell 
      population (HRCs) from bone marrow (BM) and spleen of C3H mice irradiated with 3 
      Gy X-rays. MATERIALS AND METHODS: 8-weeks old male C3H mice were irradiated 3Gy 
      of whole body X-ray (1 Gy/min) and mice were sacrificed at 1, 4, 8, and 26 weeks. 
      Then, HSPCs were isolated from BM of femur and spleen, the frequency of HRCs with 
      Sfpi1 gene deletion was analyzed by fluorescence in situ hybridization (FISH). 
      RESULTS AND CONCLUSIONS: The frequency of HRCs with HDSG in both BM and spleen 
      was increased 1 week after X-irradiation. Then, the frequency of HRCs with HDSG 
      in BM showed a gradual decrease from 4 to 26 weeks, whereas HRCs with HDSG in 
      spleen remained high, even at 26 weeks after X-irradiation. HDSG is less likely 
      to be eliminated, particularly in the spleen, after X-irradiation. The spleen as 
      well as BM of the femur may be major sites of rAML development.
FAU - Etani, Reo
AU  - Etani R
AD  - Laboratory of Environmental Health Science, Oita University of Nursing and Health 
      Sciences, Oita City, Japan.
FAU - Ojima, Mitsuaki
AU  - Ojima M
AD  - Laboratory of Environmental Health Science, Oita University of Nursing and Health 
      Sciences, Oita City, Japan.
FAU - Ariyoshi, Kentaro
AU  - Ariyoshi K
AD  - Institute of Radiation Emergency Medicine, Hirosaki University, Hirosaki City, 
      Japan.
AD  - Integrated Center for Science and Humanities, Fukushima Medical University, 
      Fukushima City, Japan.
FAU - Fujishima, Yohei
AU  - Fujishima Y
AD  - Institute of Radiation Emergency Medicine, Hirosaki University, Hirosaki City, 
      Japan.
AD  - Department of Radiation Biology, Tohoku University Grauduate School of Medicine, 
      Sendai City, Japan.
FAU - Kai, Michiaki
AU  - Kai M
AD  - Laboratory of Environmental Health Science, Oita University of Nursing and Health 
      Sciences, Oita City, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200806
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (proto-oncogene protein Spi-1)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/radiation effects
MH  - *Gene Deletion
MH  - Hematopoiesis/*radiation effects
MH  - Kinetics
MH  - Male
MH  - Mice
MH  - Proto-Oncogene Proteins/*deficiency/*genetics
MH  - Spleen/*cytology/radiation effects
MH  - Trans-Activators/*deficiency/*genetics
MH  - X-Rays
OTO - NOTNLM
OT  - Sfpi1 gene
OT  - Word
OT  - hematopoietic cells
OT  - radiation-induced acute myeloid leukemia (rAML)
EDAT- 2020/07/14 06:00
MHDA- 2021/05/22 06:00
CRDT- 2020/07/14 06:00
PHST- 2020/07/14 06:00 [pubmed]
PHST- 2021/05/22 06:00 [medline]
PHST- 2020/07/14 06:00 [entrez]
AID - 10.1080/09553002.2020.1793018 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2020 Sep;96(9):1119-1124. doi: 10.1080/09553002.2020.1793018. 
      Epub 2020 Aug 6.