PMID- 3265967
OWN - NLM
STAT- MEDLINE
DCOM- 19890411
LR  - 20151119
IS  - 0021-5155 (Print)
IS  - 0021-5155 (Linking)
VI  - 32
IP  - 3
DP  - 1988
TI  - Requirement of Ia-positive nylon wool adherent cells for activation of cytotoxic 
      T-lymphocytes specific to melanocyte-associated antigens in patients with 
      Vogt-Koyanage-Harada's disease.
PG  - 348-57
AB  - Involvement of Ia-positive nylon wool adherent cells (AC) for the expression of 
      cytotoxic T-lymphocyte (CTL) activity specific to melanocyte-associated antigens 
      (MAA) was studied. Although unseparated peripheral blood lymphocytes (PBL) from 
      several patients with Vogt-Koyanagi-Harada's (VKH) disease showed significant 
      cytotoxic activity against SK-MEL-28 (P-36) human melanoma cells which carried 
      cross-reactive surface antigens with normal melanocytes, the cytotoxic activity 
      of nylon wool column passed nonadherent cells (NAC) against the same target cells 
      markedly decreased in the absence of Ia-positive nylon wool AC. The reduced 
      cytotoxic activity of the NAC was reconstituted not only by the addition of 
      Ia-positive AC from the same VKH patient, but also by the addition of allogeneic 
      IA-positive AC mismatched with human leukocyte antigen (HLA) complex-DR region 
      haplotype. Moreover, the cytotoxic activity of nylon wool NAC was augmented by 
      the addition of interleukins instead of DR-positive AC. The data indicate that 
      the primed CTL in patients with VKH would be stimulated nonspecifically by 
      factors from Ia-positive cells, presumably to differentiate the precursor CTL 
      (CTL in memory state) into mature CTL (effector cells).
FAU - Maezawa, N
AU  - Maezawa N
AD  - Ophthalmic Clinic, Showa-Inan General Hospital, Nagano, Japan.
FAU - Yano, A
AU  - Yano A
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Ophthalmol
JT  - Japanese journal of ophthalmology
JID - 0044652
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-1)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal
MH  - Antigen-Presenting Cells/immunology
MH  - Cell Adhesion
MH  - Cell Communication
MH  - Cell Differentiation
MH  - Cell Fractionation
MH  - Cells, Cultured
MH  - Histocompatibility Antigens Class II/immunology
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Melanocytes/immunology
MH  - Middle Aged
MH  - T-Lymphocytes, Cytotoxic/*immunology
MH  - Uveitis/*immunology
MH  - Uveomeningoencephalitic Syndrome/*immunology
EDAT- 1988/01/01 00:00
MHDA- 1988/01/01 00:01
CRDT- 1988/01/01 00:00
PHST- 1988/01/01 00:00 [pubmed]
PHST- 1988/01/01 00:01 [medline]
PHST- 1988/01/01 00:00 [entrez]
PST - ppublish
SO  - Jpn J Ophthalmol. 1988;32(3):348-57.