PMID- 32662128
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1745-4514 (Electronic)
IS  - 0145-8884 (Linking)
VI  - 44
IP  - 9
DP  - 2020 Sep
TI  - A novel di-peptide Met-Glu from collagen hydrolysates inhibits platelet 
      aggregation and thrombus formation via regulation of Gq-mediated signaling.
PG  - e13352
LID - 10.1111/jfbc.13352 [doi]
AB  - Increasing evidence has shown that collagen peptides had various biological 
      activities. In this study, a novel antiplatelet peptide Met-Glu (ME) was 
      separated and identified from silver carp skin by YMC ODS-A C18 separation and 
      ESI-MS/MS analysis. Peptide ME inhibited platelet aggregation and secretion of 
      platelet granules induced by ADP, thrombin and collagen, and significantly 
      attenuated ferric chloride-induced thrombus formation in rats. It did not prolong 
      the bleeding time in mice even at the dose of 300 mumol/kg body weight that showed 
      potent anti-thrombosis effects. Additionally, peptide ME targeted at Gq-protein 
      to downregulate the phosphorylation of PLCbeta, an important upstream effector of 
      PI3K/Akt and Erk/MAPK signaling to inhibit intracellular calcium ion 
      mobilization. These results suggest that peptide ME inhibited thrombosis in vivo 
      and inhibited Gq-mediated signaling in platelets, indicating the possibility that 
      ME could potentially be developed as a novel therapeutic agent in the prevention 
      and treatment of thrombotic diseases. PRACTICAL APPLICATIONS: Cardiovascular 
      diseases (CVDs) are the leading cause of mortality and morbidity worldwide. The 
      proximal cause of CVDs is intravascular thrombosis formation, which mostly 
      results from platelet activation, aggregation, and granules secretion. 
      Traditional drugs in the prevention of thrombotic disease, such as aspirin and 
      clopidogrel, are still limited for their side effects, especially bleeding 
      complications. Collagen is a natural source for bioactive peptides and our 
      previous study has shown that collagen peptides could inhibit platelet 
      aggregation in vitro. Understanding the mechanism of collagen peptides on 
      regulation of platelet activation and their in vivo anti-thrombosis activities 
      were important for the development of novel-specific medical food in the 
      prevention of thrombotic diseases.
CI  - (c) 2020 Wiley Periodicals LLC.
FAU - Yang, Yijie
AU  - Yang Y
AD  - College of Food Science and Nutritional Engineering, China Agricultural 
      University, Beijing, China.
FAU - Song, Hongdong
AU  - Song H
AD  - College of Food Science and Nutritional Engineering, China Agricultural 
      University, Beijing, China.
FAU - Wang, Bo
AU  - Wang B
AD  - College of Food Science and Nutritional Engineering, China Agricultural 
      University, Beijing, China.
FAU - Tian, Qi
AU  - Tian Q
AD  - College of Food Science and Nutritional Engineering, China Agricultural 
      University, Beijing, China.
FAU - Li, Bo
AU  - Li B
AUID- ORCID: 0000-0003-2726-2900
AD  - College of Food Science and Nutritional Engineering, China Agricultural 
      University, Beijing, China.
AD  - Key Laboratory of Functional Dairy, Ministry of Education, Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200713
PL  - United States
TA  - J Food Biochem
JT  - Journal of food biochemistry
JID - 7706045
RN  - 0 (Dipeptides)
RN  - 0 (Peptides)
RN  - 0 (Platelet Aggregation Inhibitors)
RN  - 0 (methionylglutamic acid)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Collagen
MH  - Dipeptides
MH  - Mice
MH  - Peptides/pharmacology
MH  - Phosphatidylinositol 3-Kinases
MH  - *Platelet Aggregation
MH  - Platelet Aggregation Inhibitors/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tandem Mass Spectrometry
MH  - *Thrombosis/drug therapy/prevention & control
OTO - NOTNLM
OT  - Gq signaling
OT  - anti-thrombosis
OT  - antiplatelet
OT  - collagen peptide
EDAT- 2020/07/15 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/07/15 06:00
PHST- 2020/03/31 00:00 [received]
PHST- 2020/05/13 00:00 [revised]
PHST- 2020/05/31 00:00 [accepted]
PHST- 2020/07/15 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/07/15 06:00 [entrez]
AID - 10.1111/jfbc.13352 [doi]
PST - ppublish
SO  - J Food Biochem. 2020 Sep;44(9):e13352. doi: 10.1111/jfbc.13352. Epub 2020 Jul 13.