PMID- 32662451
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 17
DP  - 2020 Aug 24
TI  - Investigation of heart lipid changes in acute beta-AR activation-induced sudden 
      cardiac death by time-of-flight secondary ion mass spectrometry.
PG  - 5889-5896
LID - 10.1039/d0an00768d [doi]
AB  - Sudden cardiac death (SCD), one of the most common causes of human death, has 
      become a major health and social problem. The postmortem diagnosis and prevention 
      of SCD remain primary challenges in the medical community due to the lack of 
      reliable diagnostic biomarkers. Time-of-flight secondary ion mass spectrometry 
      (ToF-SIMS) is a sensitive surface analysis technique for analyzing tissue slices 
      with high spatial resolution. Here, ToF-SIMS followed by principal component 
      analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were 
      employed to study the SCD mouse myocardium samples and obtain high-resolution 
      chemical mappings and mass spectra. Distinction between normal control (NC) and 
      acute beta-adrenergic receptor (beta-AR) activation-induced SCD groups was primarily 
      due to the changes of diacylglycerols (DAG), phosphatidylcholine (PC), 
      lysophosphatidylcholine (LysoPC) and sphingomyelin (SM) in positive mode. 
      Meanwhile, chemical mapping of the myocardium showed different lipid 
      distributions in the SCD mouse myocardium. The metabolite alterations in mouse 
      models were further verified by analyzing the heart tissue sections of a 
      28-year-old woman that died from isoprenaline injection. This pilot study 
      demonstrated the significance of ToF-SIMS in characterizing the lipid variations 
      in SCD heart tissues and might contribute to the postmortem diagnosis of SCD, the 
      discovery of molecule candidates to discriminate the progression of SCD and the 
      understanding of the potential postmortem diagnostic significance of SCD.
FAU - Lou, Jia-Qian
AU  - Lou JQ
AD  - Department of Forensic Medicine, Nanjing Medical University, Nanjing, China. 
      fchen@njmu.edu.cn.
FAU - Cao, Yue
AU  - Cao Y
FAU - Yu, You-Jia
AU  - Yu YJ
FAU - Hu, Li
AU  - Hu L
FAU - Mao, Zheng-Sheng
AU  - Mao ZS
FAU - Huang, Ping
AU  - Huang P
FAU - Hua, Xin
AU  - Hua X
FAU - Chen, Feng
AU  - Chen F
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Receptors, Adrenergic, beta)
SB  - IM
EIN - Analyst. 2020 Oct 26;145(21):7071. PMID: 32902521
MH  - Animals
MH  - Death, Sudden, Cardiac/etiology
MH  - Humans
MH  - Mice
MH  - Pilot Projects
MH  - Principal Component Analysis
MH  - *Receptors, Adrenergic, beta
MH  - *Spectrometry, Mass, Secondary Ion
EDAT- 2020/07/15 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/07/15 06:00
PHST- 2020/07/15 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/07/15 06:00 [entrez]
AID - 10.1039/d0an00768d [doi]
PST - ppublish
SO  - Analyst. 2020 Aug 24;145(17):5889-5896. doi: 10.1039/d0an00768d.