PMID- 32664187
OWN - NLM
STAT- MEDLINE
DCOM- 20210412
LR  - 20210412
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 10
IP  - 7
DP  - 2020 Jul 10
TI  - Apoptosis-Inducing Factor, Mitochondrion-Associated 3 (AIFM3) Protein Level in 
      the Sera as a Prognostic Marker of Cholangiocarcinoma Patients.
LID - 10.3390/biom10071021 [doi]
LID - 1021
AB  - Prognosis of cholangiocarcinoma (CCA) patients is absolutely poor. Since 
      improvement of prognosis and/or response to treatment by personalized and 
      precision treatments requires earlier and precise diagnostic markers, discovery 
      of prognostic markers attracts more attention. Apoptosis-inducing factor, 
      mitochondrion-associated 3 (AIFM3) is highly expressed in several cancers 
      including CCA. The present study investigated whether the serum AIFM3 level can 
      be used as a potential marker for CCA prognosis. For this purpose, we first 
      determined secretory protein nature of AIFM3 using bioinformatic tools. The 
      results show that although AIFM3 lacks signal peptide, it can be secreted into 
      plasma/serum via an unconventional pathway. Then, the AIFM3 levels in the sera of 
      141 CCA patients and 70 healthy controls (HC) were measured using a 
      semi-quantitative dot blot assay. The results show that the AIFM3 level in the 
      sera of CCA group was significantly higher than that of HC. When correlation 
      between serum AIFM3 levels and the clinicopathological parameters of CCA patients 
      were examined, serum AIFM3 levels correlated significantly with lymph node 
      metastasis, age, and the patients' overall survival (OS). Higher AIFM3 levels 
      were significantly associated with shorter OS, and only AIFM3 was an independent 
      prognostic marker for CCA. In conclusion, AIFM3 can be used as a prognostic 
      marker for CCA.
FAU - Chua-On, Daraporn
AU  - Chua-On D
AD  - Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of 
      Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Proungvitaya, Tanakorn
AU  - Proungvitaya T
AD  - Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of 
      Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Tummanatsakun, Doungdean
AU  - Tummanatsakun D
AD  - Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of 
      Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Techasen, Anchalee
AU  - Techasen A
AD  - Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of 
      Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
FAU - Limpaiboon, Temduang
AU  - Limpaiboon T
AD  - Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of 
      Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
FAU - Roytrakul, Sittiruk
AU  - Roytrakul S
AUID- ORCID: 0000-0003-3696-8390
AD  - National Center for Genetic Engineering and Biotechnology, National Science and 
      Technology Development Agency, Pathumthani 12120, Thailand.
FAU - Wongkham, Sopit
AU  - Wongkham S
AD  - Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Wongkham, Chaisiri
AU  - Wongkham C
AUID- ORCID: 0000-0002-5167-211X
AD  - Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Somintara, Ongart
AU  - Somintara O
AD  - Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Sangkhamanon, Sakkarn
AU  - Sangkhamanon S
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Proungvitaya, Siriporn
AU  - Proungvitaya S
AUID- ORCID: 0000-0002-2170-4934
AD  - Centre of Research and Development of Medical Diagnostic Laboratories, Faculty of 
      Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
LA  - eng
GR  - 61003505/Khon Kean University/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200710
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (AIFM3 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Mitochondrial Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bile Duct Neoplasms/blood/*mortality
MH  - Biomarkers, Tumor/*blood
MH  - Case-Control Studies
MH  - Cholangiocarcinoma/blood/*mortality
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Mitochondrial Proteins/*blood
MH  - Prognosis
MH  - Survival Analysis
MH  - *Up-Regulation
MH  - Young Adult
PMC - PMC7408035
OTO - NOTNLM
OT  - AIFM3
OT  - CCA
OT  - dot blot
OT  - prognostic marker
OT  - serum
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/16 06:00
MHDA- 2021/04/13 06:00
PMCR- 2020/07/01
CRDT- 2020/07/16 06:00
PHST- 2020/06/11 00:00 [received]
PHST- 2020/07/05 00:00 [revised]
PHST- 2020/07/07 00:00 [accepted]
PHST- 2020/07/16 06:00 [entrez]
PHST- 2020/07/16 06:00 [pubmed]
PHST- 2021/04/13 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - biom10071021 [pii]
AID - biomolecules-10-01021 [pii]
AID - 10.3390/biom10071021 [doi]
PST - epublish
SO  - Biomolecules. 2020 Jul 10;10(7):1021. doi: 10.3390/biom10071021.