PMID- 32664305
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 9
IP  - 7
DP  - 2020 Jul 10
TI  - Circulating miRNAs as a Predictive Biomarker of the Progression from Prediabetes 
      to Diabetes: Outcomes of a 5-Year Prospective Observational Study.
LID - 10.3390/jcm9072184 [doi]
LID - 2184
AB  - Due to a global increase in the prevalence of type 2 diabetes mellitus (T2DM), 
      there is an urgent need for early identification of prediabetes, as these people 
      have the highest risk of developing diabetes. Circulating miRNAs have shown 
      potential as progression biomarkers in other diseases. This study aimed to 
      conduct a baseline comparison of serum-circulating miRNAs in prediabetic 
      individuals, with the distinction between those who later progressed to T2DM and 
      those who did not. The expression levels of 798 miRNAs using NanoString 
      technology were examined. Spearman correlation, receiver operating characteristic 
      (ROC) curve analysis, and logistic regression modeling were performed. Gene 
      ontology (GO) and canonical pathway analysis were used to explore the biological 
      functions of the miRNA target genes. The study revealed that three miRNAs were 
      upregulated in the serum samples of patients who later progressed to T2DM. 
      Pathway analysis showed that the miRNA target genes were mainly significantly 
      enriched in neuronal NO synthase (nNOS) signaling in neurons, amyloid processing, 
      and hepatic cholestasis. ROC analysis demonstrated that miR-491-5p, miR-1307-3p, 
      and miR-298 can be introduced as a diagnostic tool for the prediction of T2DM 
      (area under the curve (AUC) = 94.0%, 88.0%, and 84.0%, respectively). Validation 
      by real-time quantitative polymerase chain reaction (qRT-PCR) confirmed our 
      findings. The results suggest that circulating miRNAs can potentially be used as 
      predictive biomarkers of T2DM in prediabetic patients.
FAU - Sidorkiewicz, Iwona
AU  - Sidorkiewicz I
AUID- ORCID: 0000-0003-0254-3095
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
FAU - Niemira, Magdalena
AU  - Niemira M
AUID- ORCID: 0000-0002-0701-4961
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
FAU - Maliszewska, Katarzyna
AU  - Maliszewska K
AUID- ORCID: 0000-0001-5721-2571
AD  - Department of Endocrinology, Diabetology and Internal Medicine, Medical 
      University of Bialystok, 15-276 Bialystok, Poland.
FAU - Erol, Anna
AU  - Erol A
AUID- ORCID: 0000-0002-9932-5176
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
FAU - Bielska, Agnieszka
AU  - Bielska A
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
FAU - Szalkowska, Anna
AU  - Szalkowska A
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
FAU - Adamska-Patruno, Edyta
AU  - Adamska-Patruno E
AUID- ORCID: 0000-0002-8805-0744
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
FAU - Szczerbinski, Lukasz
AU  - Szczerbinski L
AUID- ORCID: 0000-0002-6201-0605
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
AD  - Department of Endocrinology, Diabetology and Internal Medicine, Medical 
      University of Bialystok, 15-276 Bialystok, Poland.
FAU - Gorska, Maria
AU  - Gorska M
AD  - Department of Endocrinology, Diabetology and Internal Medicine, Medical 
      University of Bialystok, 15-276 Bialystok, Poland.
FAU - Kretowski, Adam
AU  - Kretowski A
AUID- ORCID: 0000-0002-4522-4978
AD  - Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, 
      Poland.
AD  - Department of Endocrinology, Diabetology and Internal Medicine, Medical 
      University of Bialystok, 15-276 Bialystok, Poland.
LA  - eng
PT  - Journal Article
DEP - 20200710
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC7408684
OTO - NOTNLM
OT  - biomarker
OT  - diabetes
OT  - miRNA
OT  - prediabetes
OT  - serum profiling
COIS- The authors declare no conflicts of interest.
EDAT- 2020/07/16 06:00
MHDA- 2020/07/16 06:01
PMCR- 2020/07/10
CRDT- 2020/07/16 06:00
PHST- 2020/06/10 00:00 [received]
PHST- 2020/07/01 00:00 [revised]
PHST- 2020/07/07 00:00 [accepted]
PHST- 2020/07/16 06:00 [entrez]
PHST- 2020/07/16 06:00 [pubmed]
PHST- 2020/07/16 06:01 [medline]
PHST- 2020/07/10 00:00 [pmc-release]
AID - jcm9072184 [pii]
AID - jcm-09-02184 [pii]
AID - 10.3390/jcm9072184 [doi]
PST - epublish
SO  - J Clin Med. 2020 Jul 10;9(7):2184. doi: 10.3390/jcm9072184.