PMID- 32665300
OWN - NLM
STAT- MEDLINE
DCOM- 20211015
LR  - 20211015
IS  - 2573-7732 (Electronic)
IS  - 2573-7732 (Linking)
VI  - 4
IP  - 7
DP  - 2020 Jul 14
TI  - Bone Marrow-Derived Dendritic Cell Cultures from RAG(-/-) Mice Include 
      IFN-gamma-Producing NK Cells.
PG  - 415-419
LID - 10.4049/immunohorizons.2000011 [doi]
AB  - Dendritic cells (DCs) play a key role in the initiation of an immune response and 
      are known as "professional" APCs because of their ability to activate naive T 
      cells. A widely used method to generate DCs in vitro is to culture bone marrow 
      (BM) cells or blood monocytes in the presence of GM-CSF and IL-4. In this study, 
      we show that a small population of NK cells residing in the BM of RAG(-/-), but 
      not RAG(-/-) gamma(c) chain(-/-) mice, remain in the DC culture and is the source of 
      IFN-gamma produced after stimulation with LPS. These cells, which may represent early 
      promoters of LPS-induced responses, have to be taken into account when 
      interpreting experiments using BM-derived DCs.
FAU - Abdi, Kaveh
AU  - Abdi K
AUID- ORCID: 0000-0002-9763-3757
AD  - Laboratory of Immunogenetics, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Rockville, MD 20852; 
      kabdi@niaid.nih.gov.
FAU - Thomas, L Michael
AU  - Thomas LM
AD  - Laboratory of Immunogenetics, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Rockville, MD 20852.
FAU - Laky, Karen
AU  - Laky K
AUID- ORCID: 0000-0002-8197-3941
AD  - Laboratory of Allergic Diseases, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Bethesda, MD 20892; and.
FAU - Abshari, Mehrnoosh
AU  - Abshari M
AUID- ORCID: 0000-0003-4217-0625
AD  - National Institute of Dental and Craniofacial Research, National Institutes of 
      Health, Bethesda, MD 20892.
FAU - Matzinger, Polly
AU  - Matzinger P
AD  - Laboratory of Immunogenetics, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Rockville, MD 20852.
FAU - Long, Eric O
AU  - Long EO
AUID- ORCID: 0000-0002-7793-3728
AD  - Laboratory of Immunogenetics, National Institute of Allergy and Infectious 
      Diseases, National Institutes of Health, Rockville, MD 20852.
LA  - eng
GR  - Z99 AI999999/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20200714
PL  - United States
TA  - Immunohorizons
JT  - ImmunoHorizons
JID - 101708159
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 128559-51-3 (RAG-1 protein)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects/immunology
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/*cytology/drug effects/immunology
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-4/pharmacology
MH  - Killer Cells, Natural/*immunology
MH  - Lipopolysaccharides/toxicity
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Monocytes/immunology
MH  - T-Lymphocytes/cytology/drug effects/immunology
PMC - PMC7454002
MID - NIHMS1621511
COIS- Conflict of interest The authors have no financial conflict of interest.
EDAT- 2020/07/16 06:00
MHDA- 2021/10/16 06:00
PMCR- 2020/08/28
CRDT- 2020/07/16 06:00
PHST- 2020/03/10 00:00 [received]
PHST- 2020/06/25 00:00 [accepted]
PHST- 2020/07/16 06:00 [entrez]
PHST- 2020/07/16 06:00 [pubmed]
PHST- 2021/10/16 06:00 [medline]
PHST- 2020/08/28 00:00 [pmc-release]
AID - 4/7/415 [pii]
AID - 10.4049/immunohorizons.2000011 [doi]
PST - epublish
SO  - Immunohorizons. 2020 Jul 14;4(7):415-419. doi: 10.4049/immunohorizons.2000011.