PMID- 3266663
OWN - NLM
STAT- MEDLINE
DCOM- 19890526
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 9
IP  - 5
DP  - 1988 Sep-Oct
TI  - Urotensin I- and CRF-like peptides in Catostomus commersoni brain and 
      pituitary--HPLC and RIA characterization.
PG  - 1043-8
AB  - Two distinct neuronal systems, containing urotensin I-like immunoreactivity 
      (UI-LI) and CRF-LI respectively, have previously been demonstrated 
      immunocytochemically in the brain and pituitary of the teleost Catostomus 
      commersoni. In the present studies, we used HPLC followed by UI- and CRF-RIA to 
      further characterize these UI- and CRF-LI substances. HPLC of Catostomus brain 
      extracts (including extracts modified by dilute H2O2 oxidation or dilute acid 
      cleavage) suggests that the major form of brain UI-LI is very likely identical to 
      the previously characterized urophysial UI; the small amount of pituitary UI-LI 
      is not yet fully characterized. The behavior of brain CRF-LI on reversed phase 
      (RP) extraction suggests that it may exist largely in precursor form. Pituitary 
      CRF-LI, however, behaved as expected for a peptide on RP extraction, and eluted 
      from HPLC as essentially a single (though somewhat broad) peak. The HPLC behavior 
      of pituitary CRF-LI, and its crossreactivity with various CRF antisera, suggest 
      that it represents authentic fish CRF, apparently similar to (but not identical 
      with) human/rat CRF.
FAU - McMaster, D
AU  - McMaster D
AD  - Department of Pharmacology and Therapeutics, University of Calgary, Faculty of 
      Medicine, Alberta, Canada.
FAU - Lederis, K
AU  - Lederis K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Peptides)
RN  - 0 (Urotensins)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - 9047-54-5 (urotensin I)
SB  - IM
MH  - Animals
MH  - *Brain Chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Corticotropin-Releasing Hormone/*analysis
MH  - Cross Reactions
MH  - Cypriniformes/*metabolism
MH  - Organ Specificity
MH  - Peptides/*analysis
MH  - Pituitary Gland/*analysis
MH  - Radioimmunoassay/methods
MH  - Urotensins/*analysis
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 0196-9781(88)90086-1 [pii]
AID - 10.1016/0196-9781(88)90086-1 [doi]
PST - ppublish
SO  - Peptides. 1988 Sep-Oct;9(5):1043-8. doi: 10.1016/0196-9781(88)90086-1.