PMID- 32668664
OWN - NLM
STAT- MEDLINE
DCOM- 20210315
LR  - 20210315
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 11
IP  - 7
DP  - 2020 Jul 13
TI  - Satellitome Analysis in the Ladybird Beetle Hippodamia variegata (Coleoptera, 
      Coccinellidae).
LID - 10.3390/genes11070783 [doi]
LID - 783
AB  - Hippodamia variegata is one of the most commercialized ladybirds used for the 
      biological control of aphid pest species in many economically important crops. 
      This species is the first Coccinellidae whose satellitome has been studied by 
      applying new sequencing technologies and bioinformatics tools. We found that 47% 
      of the H. variegata genome is composed of repeated sequences. We identified 30 
      satellite DNA (satDNA) families with a median intragenomic divergence of 5.75% 
      and A+T content between 45.6% and 74.7%. This species shows satDNA families with 
      highly variable sizes although the most common size is 100-200 bp. However, we 
      highlight the existence of a satDNA family with a repeat unit of 2 kb, the 
      largest repeat unit described in Coleoptera. PCR amplifications for fluorescence 
      in situ hybridization (FISH) probe generation were performed for the four most 
      abundant satDNA families. FISH with the most abundant satDNA family as a probe 
      shows its pericentromeric location on all chromosomes. This location is 
      coincident with the heterochromatin revealed by C-banding and DAPI staining, also 
      analyzed in this work. Hybridization signals for other satDNA families were 
      located only on certain bivalents and the X chromosome. These satDNAs could be 
      very useful as chromosomal markers due to their reduced location.
FAU - Mora, Pablo
AU  - Mora P
AUID- ORCID: 0000-0001-7967-3379
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain.
FAU - Vela, Jesus
AU  - Vela J
AUID- ORCID: 0000-0003-2699-3031
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain.
FAU - Ruiz-Ruano, Francisco J
AU  - Ruiz-Ruano FJ
AUID- ORCID: 0000-0002-5391-301X
AD  - Department of Organismal Biology, Systematic Biology, Evolutionary Biology 
      Centre, Uppsala University, SE-752 36 Uppsala, Sweden.
FAU - Ruiz-Mena, Areli
AU  - Ruiz-Mena A
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain.
FAU - Montiel, Eugenia E
AU  - Montiel EE
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain.
FAU - Palomeque, Teresa
AU  - Palomeque T
AUID- ORCID: 0000-0002-7144-4931
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain.
FAU - Lorite, Pedro
AU  - Lorite P
AUID- ORCID: 0000-0002-9692-5870
AD  - Department of Experimental Biology, Genetic Area, University of Jaen, 23071 Jaen, 
      Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200713
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (DNA, Satellite)
RN  - 0 (Heterochromatin)
SB  - IM
MH  - Animals
MH  - Aphids/genetics/pathogenicity
MH  - Chromosome Mapping/methods
MH  - Coleoptera/*genetics/pathogenicity
MH  - DNA, Satellite/*genetics
MH  - *Evolution, Molecular
MH  - Heterochromatin/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Pest Control
MH  - *Phylogeny
MH  - Repetitive Sequences, Nucleic Acid/genetics
PMC - PMC7397073
OTO - NOTNLM
OT  - Coccinellidae
OT  - Coleoptera
OT  - Hippodamia variegata
OT  - RepeatExplorer
OT  - in situ hybridization
OT  - ladybird beetle
OT  - satellite DNA
OT  - satellitome
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/17 06:00
MHDA- 2021/03/16 06:00
PMCR- 2020/07/01
CRDT- 2020/07/17 06:00
PHST- 2020/06/18 00:00 [received]
PHST- 2020/07/09 00:00 [revised]
PHST- 2020/07/09 00:00 [accepted]
PHST- 2020/07/17 06:00 [entrez]
PHST- 2020/07/17 06:00 [pubmed]
PHST- 2021/03/16 06:00 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - genes11070783 [pii]
AID - genes-11-00783 [pii]
AID - 10.3390/genes11070783 [doi]
PST - epublish
SO  - Genes (Basel). 2020 Jul 13;11(7):783. doi: 10.3390/genes11070783.