PMID- 32668963
OWN - NLM
STAT- MEDLINE
DCOM- 20201020
LR  - 20211204
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 48
IP  - 5
DP  - 2020
TI  - 4-Acetylantrocamol LT3, a New Ubiquinone from Antrodia cinnamomea, Inhibits 
      Hepatocellular Carcinoma HepG2 Cell Growth by Targeting YAP/TAZ, mTOR, and 
      WNT/beta-Catenin Signaling.
PG  - 1243-1261
LID - 10.1142/S0192415X20500615 [doi]
AB  - 4-acetylantrocamol LT3 (4AALT3), a new ubiquinone from the mycelium of Antrodia 
      cinnamomea (Polyporaceae), has been recently shown to possess anticancer 
      activity. However, the detailed mechanisms of such action remain unclear. In this 
      study, the molecular mechanisms of 4AALT3 on hepatocellular carcinoma cells (HCC) 
      were investigated. Human hepatocellular carcinoma cell line HepG2 cells were 
      treated with concentrations of 4AALT3. Cell viability, colony formation, and the 
      underlying mechanisms were then analyzed by CCK-8, colony formation, qPCR, and 
      Western blotting assays. We found that 4AALT3 significantly decreased cell 
      viability and colony formation in a dose-dependent manner. Accordingly, 4AALT3 
      significantly decreased protein levels of cyclin B, E1, D1, and D3, thereby 
      facilitating cell cycle arrest. In addition, 4AALT3 significantly suppressed the 
      nuclear localization of Yes-associated protein (YAP)/transcriptional co-activator 
      with PDZ-binding motif (TAZ), mammalian target of rapamycin (mTOR), and 
      WNT/[Formula: see text]-catenin signaling pathways, all of which are well-known 
      signaling pathways that contribute to the malignant properties of HCC. These 
      effects are associated with activation of 5' AMP-activated protein kinase (AMPK) 
      and autophagy. Our findings indicate that 4AALT3 exerts inhibitory effects on 
      HepG2 cell growth via multiple signaling pathways and may be a potential agent 
      for HCC therapy.
FAU - Chen, Yen-Lin
AU  - Chen YL
AD  - Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
AD  - Department of Radiology, Taoyuan Armed Forces General Hospital, National Defense 
      Medical Center, Taipei, Taiwan.
FAU - Yen, I-Chuan
AU  - Yen IC
AD  - School of Pharmacy, National Defense Medical Center, Taipei, Taiwan.
FAU - Lin, Kuen-Tze
AU  - Lin KT
AD  - Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
AD  - Department of Radiation Oncology, Tri-Service General Hospital, National Defense 
      Medical Center, Taipei, Taiwan.
FAU - Lai, Feng-Yi
AU  - Lai FY
AD  - Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical 
      Center, Taipei, Taiwan.
FAU - Lee, Shih-Yu
AU  - Lee SY
AD  - Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
AD  - Graduate Institute of Aerospace and Undersea Medicine, National Defense Medical 
      Center, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Transcription Factors)
RN  - 0 (Wnt Proteins)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
RN  - 0 (antrocamol)
RN  - 0 (beta Catenin)
RN  - 1339-63-5 (Ubiquinone)
RN  - EC 2.3.- (Acyltransferases)
RN  - EC 2.3.1.- (TAFAZZIN protein, human)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Acyltransferases
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - Antineoplastic Agents, Phytogenic
MH  - Antrodia/*chemistry
MH  - Autophagy/drug effects/genetics
MH  - Carcinoma, Hepatocellular/*genetics/*pathology
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver Neoplasms/*genetics/*pathology
MH  - Signal Transduction/*drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Transcription Factors/*metabolism
MH  - Ubiquinone/*analogs & derivatives/isolation & purification/*pharmacology
MH  - Wnt Proteins/*metabolism
MH  - YAP-Signaling Proteins
MH  - beta Catenin/*metabolism
OTO - NOTNLM
OT  - 4-Acetylantrocamol LT3
OT  - Autophagy
OT  - Hepatocellular Carcinoma Cells
OT  - Wnt/[Formula: see text]-Catenin, mTOR
OT  - YAP/TAZ
EDAT- 2020/07/17 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/07/17 06:00
PHST- 2020/07/17 06:00 [entrez]
PHST- 2020/07/17 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 10.1142/S0192415X20500615 [doi]
PST - ppublish
SO  - Am J Chin Med. 2020;48(5):1243-1261. doi: 10.1142/S0192415X20500615.