PMID- 32670817
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231111
IS  - 2225-4110 (Print)
IS  - 2225-4110 (Electronic)
IS  - 2225-4110 (Linking)
VI  - 10
IP  - 3
DP  - 2020 May
TI  - Curative effect of xanthohumol supplementation during liver fluke-associated 
      cholangiocarcinogenesis: Potential involvement of autophagy.
PG  - 230-235
LID - 10.1016/j.jtcme.2019.02.001 [doi]
AB  - Xanthohumol (XH), a plant flavonoid, was shown to attenuate cholangiocarcinoma 
      (CCA) development induced by the liver fluke Opisthorchis viverrini (Ov) and 
      N-dinitrosomethylamine (NDMA) in the hamster model. We investigated the possible 
      involvement of autophagy, a self-degrading process dysregulated in cancer, in XH 
      chemotherapeutic effect. During cholangiocarcinogenesis, the expression of LC3 
      (an autophagic marker) was increased in the precancerous stage and decreased in 
      the cancerous stage. The XH-treated ON (Ov plus NDMA) group showed retarded 
      progression of CCA along with increased expression of LC3. The possible relation 
      between autophagy and cell death was investigated in cultured human CCA cells. XH 
      induced apoptosis associated with reduced expression of BCL-2 and increased 
      expression of BAX. In parallel, XH induced the autophagy flux, as testified by 
      increased LC3-II and decreased p62, along with induction of BECLIN1 and Vps34. 
      Inhibition of BECLIN1-dependent autophagy greatly limited XH toxicity in CCA 
      cells. These data suggest that XH attenuates the development of CCA through 
      overstimulation of autophagy which then precipitates apoptosis.
CI  - (c) 2019 Center for Food and Biomolecules, National Taiwan University. Production 
      and hosting by Elsevier Taiwan LLC.
FAU - Thongchot, Suyanee
AU  - Thongchot S
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, 123 
      Mitraparp Highway, Khon Kaen, 40002, Thailand.
AD  - Laboratory of Molecular Pathology, Department of Health Sciences, Universita del 
      Piemonte Orientale "A. Avogadro", Via Solaroli 17, 28100, Novara, Italy.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Thanee, Malinee
AU  - Thanee M
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, 123 
      Mitraparp Highway, Khon Kaen, 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Loilome, Watcharin
AU  - Loilome W
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, 123 
      Mitraparp Highway, Khon Kaen, 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Techasen, Anchalee
AU  - Techasen A
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
AD  - Faculty of Associated Medical Sciences, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Boonmars, Thidarat
AU  - Boonmars T
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
AD  - Department of Parasitology, Faculty of Medicine, Khon Kaen University, 123 
      Mitraparp Highway, Khon Kaen, 40002, Thailand.
FAU - Sa-Ngiamwibool, Prakasit
AU  - Sa-Ngiamwibool P
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
AD  - Department of Pathology, Faculty of Medicine, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Titapun, Attapol
AU  - Titapun A
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
AD  - Department of Surgery, Faculty of Medicine, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Yongvanit, Puangrat
AU  - Yongvanit P
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
FAU - Isidoro, Ciro
AU  - Isidoro C
AD  - Laboratory of Molecular Pathology, Department of Health Sciences, Universita del 
      Piemonte Orientale "A. Avogadro", Via Solaroli 17, 28100, Novara, Italy.
FAU - Namwat, Nisana
AU  - Namwat N
AD  - Department of Biochemistry, Faculty of Medicine, Khon Kaen University, 123 
      Mitraparp Highway, Khon Kaen, 40002, Thailand.
AD  - Cholangiocarcinoma Research Institute, Khon Kaen University, 123 Mitraparp 
      Highway, Khon Kaen, 40002, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20190210
PL  - Netherlands
TA  - J Tradit Complement Med
JT  - Journal of traditional and complementary medicine
JID - 101605474
PMC - PMC7340870
OTO - NOTNLM
OT  - Apoptosis
OT  - BECLIN1
OT  - CCA, cholangiocarcinoma
OT  - CIQ, chloroquine
OT  - Cancer
OT  - Cholangiocarcinoma
OT  - Flavonoids
OT  - Herbal medicine
OT  - LC3
OT  - NDMA, N-dinitrosomethylamine
OT  - OV, Opisthorchis viverrini
OT  - Opistorchis viverrini
OT  - SP-1, Spautin-1
OT  - Spautin
OT  - Therapy
OT  - XH, Xanthohumol
EDAT- 2019/02/10 00:00
MHDA- 2019/02/10 00:01
PMCR- 2019/02/10
CRDT- 2020/07/17 06:00
PHST- 2018/11/19 00:00 [received]
PHST- 2019/01/29 00:00 [revised]
PHST- 2019/02/08 00:00 [accepted]
PHST- 2020/07/17 06:00 [entrez]
PHST- 2019/02/10 00:00 [pubmed]
PHST- 2019/02/10 00:01 [medline]
PHST- 2019/02/10 00:00 [pmc-release]
AID - S2225-4110(18)30859-9 [pii]
AID - 10.1016/j.jtcme.2019.02.001 [doi]
PST - epublish
SO  - J Tradit Complement Med. 2019 Feb 10;10(3):230-235. doi: 
      10.1016/j.jtcme.2019.02.001. eCollection 2020 May.