PMID- 32673675
OWN - NLM
STAT- MEDLINE
DCOM- 20201214
LR  - 20201214
IS  - 1879-2650 (Electronic)
IS  - 0005-2728 (Linking)
VI  - 1861
IP  - 11
DP  - 2020 Nov 1
TI  - Cytochrome c oxidase oxygen reduction reaction induced by cytochrome c on 
      nickel-coordination surfaces based on graphene oxide in suspension.
PG  - 148262
LID - S0005-2728(20)30112-2 [pii]
LID - 10.1016/j.bbabio.2020.148262 [doi]
AB  - BACKGROUND: The electrochemical and spectroscopic investigation of bacterial 
      electron-transfer proteins stabilized on solid state electrodes has provided an 
      effective approach for functional respiratory enzyme studies. METHODS: We assess 
      the biocompatibility of carboxylated graphene oxide (CGO) functionalized with 
      Nickel nitrilotriacetic groups (CGO-NiNTA) ccordinating His-tagged cytochrome c 
      oxidase (CcO) from Rhodobacter sphaeroides. RESULTS: Kinetic studies employing 
      UV-visible absorption spectroscopy confirmed that the immobilized CcO oxidized 
      horse-heart cytochrome c (Cyt c) albeit at a slower rate than isolated CcO. The 
      oxygen reduction reaction as catalyzed by immobilized CcO could be clearly 
      distinguished from that arising from CGO-NiNTA in the presence of Cyt c and 
      dithiothreitol (DTT) as a sacrificial reducing agent. Our findings indicate that 
      while the protein content is about 3.7 per thousand by mass with respect to the support, the 
      contribution to the oxygen consumption activity averaged at 56.3%. CONCLUSIONS: 
      The CGO-based support stabilizes the free enzyme which, while capable of Cyt c 
      oxidation, is unable to carry out oxygen consumption in solution on its own under 
      our conditions. The turnover rate for the immobilized CcO was as high as 240 O(2) 
      molecules per second per CcO unit. GENERAL SIGNIFICANCE: In vitro investigations 
      of electron flow on isolated components of bacterial electron-transfer enzymes 
      immobilized on the surface of CGO in suspension are expected to shed new light on 
      microbial bioenergetic functions, that could ultimately contribute toward the 
      improvement of performance in living organisms.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhu, Xiaoping
AU  - Zhu X
AD  - Department of Chemistry and Environmental Science, Medgar Evers College of the 
      City University of New York (CUNY), Brooklyn, NY 11225, USA.
FAU - Aoyama, Erika
AU  - Aoyama E
AD  - Department of Chemistry, The University of Utah, Salt Lake City, UT 84112, USA.
FAU - Birk, Alexander V
AU  - Birk AV
AD  - Department of Chemistry and Environmental Science, Medgar Evers College of the 
      City University of New York (CUNY), Brooklyn, NY 11225, USA; Department of 
      Biology, York College of CUNY, Jamaica, NY 11451, USA.
FAU - Onasanya, Oladapo
AU  - Onasanya O
AD  - Department of Chemistry and Environmental Science, Medgar Evers College of the 
      City University of New York (CUNY), Brooklyn, NY 11225, USA.
FAU - Carr, William H
AU  - Carr WH
AD  - Department of Biology, Medgar Evers College of the City University of New York 
      (CUNY), Brooklyn, NY 11225, USA.
FAU - Mourokh, Lev
AU  - Mourokh L
AD  - Department of Physics, Queens College of CUNY, Queens, NY 11367, USA; The 
      Graduate Center of CUNY, New York, NY 10016, USA.
FAU - Minteer, Shelley D
AU  - Minteer SD
AD  - Department of Chemistry, The University of Utah, Salt Lake City, UT 84112, USA.
FAU - Vittadello, Michele
AU  - Vittadello M
AD  - Department of Chemistry and Environmental Science, Medgar Evers College of the 
      City University of New York (CUNY), Brooklyn, NY 11225, USA; The Graduate Center 
      of CUNY, New York, NY 10016, USA. Electronic address: mvittadello@mec.cuny.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200714
PL  - Netherlands
TA  - Biochim Biophys Acta Bioenerg
JT  - Biochimica et biophysica acta. Bioenergetics
JID - 101731706
RN  - 0 (Bacterial Proteins)
RN  - 0 (Suspensions)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - 7OV03QG267 (Nickel)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Bacterial Proteins/chemistry/*metabolism
MH  - Catalysis
MH  - Cytochromes c/*metabolism
MH  - Electron Transport
MH  - Electron Transport Complex IV/chemistry/*metabolism
MH  - Graphite/*chemistry
MH  - Kinetics
MH  - Nickel/*chemistry
MH  - Oxidation-Reduction
MH  - Oxygen/*chemistry
MH  - Rhodobacter sphaeroides/*enzymology
MH  - Suspensions
OTO - NOTNLM
OT  - Electron transfer
OT  - Graphene oxide
OT  - Histidine-tagged cytochrome c oxidase
OT  - Nickel-coordination
OT  - Oxygen reduction reaction
OT  - Rhodobacter sphaeroides
COIS- Declaration of competing interest The authors declare no conflict of interest.
EDAT- 2020/07/17 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/07/17 06:00
PHST- 2020/01/04 00:00 [received]
PHST- 2020/07/01 00:00 [revised]
PHST- 2020/07/07 00:00 [accepted]
PHST- 2020/07/17 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/07/17 06:00 [entrez]
AID - S0005-2728(20)30112-2 [pii]
AID - 10.1016/j.bbabio.2020.148262 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Bioenerg. 2020 Nov 1;1861(11):148262. doi: 
      10.1016/j.bbabio.2020.148262. Epub 2020 Jul 14.