PMID- 32674394
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 10
IP  - 7
DP  - 2020 Jul 14
TI  - Cellular Responses of Human Lymphatic Endothelial Cells to Carbon Nanomaterials.
LID - 10.3390/nano10071374 [doi]
LID - 1374
AB  - One of the greatest challenges to overcome in the pursuit of the medical 
      application of carbon nanomaterials (CNMs) is safety. Particularly, when 
      considering the use of CNMs in drug delivery systems (DDSs), evaluation of safety 
      at the accumulation site is an essential step. In this study, we evaluated the 
      toxicity of carbon nanohorns (CNHs), which are potential DDSs, using human lymph 
      node endothelial cells that have been reported to accumulate CNMs, as a 
      comparison to fibrous, multi-walled carbon nanotubes (MWCNTs) and particulate 
      carbon black (CB). The effect of different surface characteristics was also 
      evaluated using two types of CNHs (untreated and oxidized). In the fibrous MWCNT, 
      cell growth suppression, as well as expression of inflammatory cytokine genes was 
      observed, as in previous reports. In contrast, no significant toxicity was 
      observed for particulate CB and CNHs, which was different from the report of CB 
      cytotoxicity in vascular endothelial cells. These results show that (1) lymph 
      endothelial cells need to be tested separately from other endothelial cells for 
      safety evaluation of nanomaterials, and (2) the potential of CNHs as DDSs.
FAU - Sano, Mahoko
AU  - Sano M
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Science and Technology, 
      Shinshu University, Nagano 390-8621, Japan.
FAU - Izumiya, Makoto
AU  - Izumiya M
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Science and Technology, 
      Shinshu University, Nagano 390-8621, Japan.
FAU - Haniu, Hisao
AU  - Haniu H
AUID- ORCID: 0000-0001-5195-9711
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Science and Technology, 
      Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Medicine, Science and 
      Technology, Shinshu University, Nagano 390-8621, Japan.
FAU - Ueda, Katsuya
AU  - Ueda K
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Medicine, Science and 
      Technology, Shinshu University, Nagano 390-8621, Japan.
FAU - Konishi, Kosuke
AU  - Konishi K
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Science and Technology, 
      Shinshu University, Nagano 390-8621, Japan.
FAU - Ishida, Haruka
AU  - Ishida H
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Biomedical Engineering Division, Graduate School of Medicine, Science and 
      Technology, Shinshu University, Nagano 390-8621, Japan.
FAU - Kuroda, Chika
AU  - Kuroda C
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
FAU - Uemura, Takeshi
AU  - Uemura T
AUID- ORCID: 0000-0001-6000-4618
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
AD  - Division of Gene Research, Research Center for Supports to Advanced Science, 
      Shinshu University, Nagano 390-8621, Japan.
FAU - Aoki, Kaoru
AU  - Aoki K
AD  - Physical and Occupational Therapy Division, Graduate School of Medicine, Shinshu 
      University, Nagano 390-8621, Japan.
FAU - Matsuda, Yoshikazu
AU  - Matsuda Y
AD  - Division of Clinical Pharmacology and Pharmaceutics, Nihon Pharmaceutical 
      University, Saitama 362-0806, Japan.
FAU - Saito, Naoto
AU  - Saito N
AD  - Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge 
      Research, Shinshu University, Nagano 390-8621, Japan.
LA  - eng
GR  - 17H01584/Japan Society for the Promotion of Science/
PT  - Journal Article
DEP - 20200714
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7407296
OTO - NOTNLM
OT  - carbon black
OT  - carbon nanohorn
OT  - cellular response
OT  - cytotoxicity
OT  - lymphatic endothelial cells
OT  - multi-walled carbon nanotube
COIS- The authors declare no conflict of interest.
EDAT- 2020/07/18 06:00
MHDA- 2020/07/18 06:01
PMCR- 2020/07/14
CRDT- 2020/07/18 06:00
PHST- 2020/06/10 00:00 [received]
PHST- 2020/07/08 00:00 [revised]
PHST- 2020/07/10 00:00 [accepted]
PHST- 2020/07/18 06:00 [entrez]
PHST- 2020/07/18 06:00 [pubmed]
PHST- 2020/07/18 06:01 [medline]
PHST- 2020/07/14 00:00 [pmc-release]
AID - nano10071374 [pii]
AID - nanomaterials-10-01374 [pii]
AID - 10.3390/nano10071374 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2020 Jul 14;10(7):1374. doi: 10.3390/nano10071374.