PMID- 32674666
OWN - NLM
STAT- MEDLINE
DCOM- 20210902
LR  - 20210902
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 29
IP  - 18
DP  - 2020 Sep 15
TI  - Combined Use of Recombinant Human BMP-7 and Osteogenic Media May Have No Ideal 
      Synergistic Effect on Leporine Bone Regeneration of Human Umbilical Cord 
      Mesenchymal Stem Cells Seeded on Nanohydroxyapatite/Collagen/Poly (l-Lactide).
PG  - 1215-1228
LID - 10.1089/scd.2020.0066 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUC-MSCs) are a promising 
      alternative source of mesenchymal stem cells (MSCs) that are enormously 
      attractive for clinical use. This study was designed to investigate the effect of 
      recombinant human bone morphogenetic protein-7 (rhBMP-7) and/or osteogenic media 
      (OMD) on bone regeneration of hUC-MSCs seeded on 
      nanohydroxyapatite/collagen/poly(l-lactide) (nHAC/PLA) in a rabbit model. The 
      characteristics of stem cells were analyzed by plastic adherence, cell phenotype, 
      and multilineage differentiation potential. Cell proliferation was examined using 
      cell counting kit-8 assay. Osteogenic differentiation was evaluated by 
      quantitative Ca(2+) concentration, PO(4)(3-) concentration, alkaline phosphatase 
      (ALP) activity, osteocalcin (OCN) secretion, and mineralized matrix formation. 
      Bone regeneration was investigated in jaw bone defect repair in rabbit by 
      microcomputed tomography, fluorescent labeling, and hematoxylin and eosin 
      staining. Except for initial stress response, OMD and OMD + rhBMP-7 inhibited the 
      proliferation of hUC-MSCs seeded on nHAC/PLA; rhBMP-7 inhibited cell 
      proliferation in the nonlogarithmic phase and attenuated the inhibitory effect of 
      OMD on cell proliferation. The inhibitory effects of OMD, rhBMP-7, and OMD + 
      rhBMP-7 on cell proliferation were ranked as OMD > OMD + rhBMP-7 > rhBMP-7. OMD, 
      rhBMP-7, and OMD + rhBMP-7 promoted Ca(2+) concentration, PO(4)(3-) 
      concentration, ALP activity, OCN secretion, and mineralized matrix formation of 
      hUC-MSCs seeded on nHAC/PLA. The promoting effects of OMD, rhBMP-7, and 
      OMD+rhBMP-7 on Ca(2+) concentration, PO(4)(3-) concentration, ALP activity, OCN 
      secretion, and mineralized matrix formation were ranked as rhBMP-7 > OMD > OMD + 
      rhBMP-7, OMD > OMD + rhBMP-7 > rhBMP-7, OMD > rhBMP-7 > OMD + rhBMP-7, rhBMP-7 > 
      OMD + rhBMP-7 > OMD, and OMD > rhBMP-7 > OMD + rhBMP-7, respectively. In rabbit 
      jaw bone defect repair, OMD, rhBMP-7, and OMD + rhBMP-7 enhanced bone 
      regeneration of hUC-MSCs seeded on nHAC/PLA, but the largest bone mineral 
      apposition rate and bone formation were presented in cultures with rhBMP-7. These 
      findings suggested that the combined use of rhBMP-7 and OMD may have no ideal 
      synergistic effect on bone regeneration of hUC-MSCs seeded on nHAC/PLA in rabbit 
      jaw bone defect.
FAU - E, Ling-Ling
AU  - E LL
AD  - Department of Chemistry, Jinan University, Guangzhou, China.
AD  - Institute of Stomatology, Chinese People's Liberation Army General Hospital, 
      Beijing, China.
FAU - Cheng, Tao
AU  - Cheng T
AD  - Institute of Stomatology, Chinese People's Liberation Army General Hospital, 
      Beijing, China.
FAU - Li, Chuan-Jie
AU  - Li CJ
AD  - Institute of Stomatology, Chinese People's Liberation Army General Hospital, 
      Beijing, China.
FAU - Zhang, Rong
AU  - Zhang R
AD  - Institute of Stomatology, Chinese People's Liberation Army General Hospital, 
      Beijing, China.
FAU - Zhang, Shuo
AU  - Zhang S
AD  - Institute of Stomatology, Chinese People's Liberation Army General Hospital, 
      Beijing, China.
FAU - Liu, Hong-Chen
AU  - Liu HC
AD  - Institute of Stomatology, Chinese People's Liberation Army General Hospital, 
      Beijing, China.
FAU - Zheng, Wen-Jie
AU  - Zheng WJ
AD  - Department of Chemistry, Jinan University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200813
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (BMP7 protein, human)
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 0 (Culture Media)
RN  - 0 (Phosphates)
RN  - 0 (Polyesters)
RN  - 0 (Recombinant Proteins)
RN  - 0 (nano-hydroxyapatite-collagen)
RN  - 104982-03-8 (Osteocalcin)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 9007-34-5 (Collagen)
RN  - 91D9GV0Z28 (Durapatite)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Morphogenetic Protein 7/*pharmacology
MH  - Bone Regeneration/*drug effects
MH  - Calcification, Physiologic/drug effects
MH  - Calcium/analysis
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Collagen/*pharmacology
MH  - Culture Media
MH  - Durapatite/*pharmacology
MH  - Female
MH  - Humans
MH  - Jaw/drug effects/pathology
MH  - Mesenchymal Stem Cells/*cytology/drug effects/ultrastructure
MH  - Osteocalcin/metabolism
MH  - *Osteogenesis
MH  - Phosphates/analysis
MH  - Polyesters/*pharmacology
MH  - Rabbits
MH  - Recombinant Proteins/*pharmacology
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - bone tissue engineering
OT  - human umbilical cord mesenchymal stem cells
OT  - nanohydroxyapatite/collagen/poly(l-lactide)
OT  - osteogenic media
OT  - recombinant human bone morphogenetic protein-7
EDAT- 2020/07/18 06:00
MHDA- 2021/09/03 06:00
CRDT- 2020/07/18 06:00
PHST- 2020/07/18 06:00 [pubmed]
PHST- 2021/09/03 06:00 [medline]
PHST- 2020/07/18 06:00 [entrez]
AID - 10.1089/scd.2020.0066 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2020 Sep 15;29(18):1215-1228. doi: 10.1089/scd.2020.0066. Epub 
      2020 Aug 13.