PMID- 32675560
OWN - NLM
STAT- MEDLINE
DCOM- 20210218
LR  - 20210218
IS  - 1473-5571 (Electronic)
IS  - 0269-9370 (Linking)
VI  - 34
IP  - 10
DP  - 2020 Aug 1
TI  - High abundance of genus Prevotella is associated with dysregulation of IFN-I and 
      T cell response in HIV-1-infected patients.
PG  - 1467-1473
LID - 10.1097/QAD.0000000000002574 [doi]
AB  - OBJECTIVE: HIV-1-associated dysbiosis is most commonly characterized by overall 
      decreased diversity, with abundance of the genus Prevotella, recently related to 
      inflammatory responses. DESIGN: A pilot study including 10 antiretroviral 
      therapy-treated HIV-1-infected men and 50 uninfected controls was performed to 
      identify the main gut dysbiosis determinants (e.g. Prevotella enrichment), that 
      may affect mucosal antiviral defenses and T cell immunity in HIV-1-infected 
      individuals. METHODS: 16rRNA gene sequencing was applied to the HIV-1-infected 
      individuals' fecal microbiota and compared with controls. Measurements of CD4 and 
      CD8 T cell activation [CD38, human leukocyte antigen (HLA)-DR, CD38 HLA-DR] and 
      frequencies of Th17, obtained from lamina propria lymphocytes isolated from five 
      different intestinal sites, were performed by flow cytometry. IFNbeta, IFNAR1 and 
      MxA gene expression level was evaluated by real-time PCR in lamina propria 
      lymphocytes. Nonparametric t tests were used for statistical analysis. RESULTS: 
      HIV-1-infected men had a significant fecal microbial communities' imbalance, 
      including different levels of genera Faecalibacterium, Prevotella, Alistipes and 
      Bacteroides, compared with controls. Notably, Prevotella abundance positively 
      correlated with frequencies of CD4 T cells expressing CD38 or HLA-DR and 
      coexpressing CD38 and HLA-DR (P < 0.05 for all these measures). The same trend 
      was observed for the activated CD8 T cells. Moreover, Prevotella levels were 
      inversely correlated with IFN-I genes (P < 0.05 for IFNbeta, IFNAR1 and MxA genes) 
      and the frequencies of Th17 cells (P < 0.05). By contrast, no statistically 
      significant correlations were observed for the remaining bacterial genera. 
      CONCLUSION: Our findings suggest that Prevotella enrichment might affect gut 
      mucosal IFN-I pathways and T cell response in HIV-1-infected patients, thus 
      contributing to immune dysfunction.
FAU - Pinacchio, Claudia
AU  - Pinacchio C
AD  - Department of Public Health and Infectious Diseases.
FAU - Scagnolari, Carolina
AU  - Scagnolari C
AD  - Laboratory of Virology, Department of Molecular Medicine, Istituto Pasteur 
      Italia, Sapienza University, Rome.
FAU - Iebba, Valerio
AU  - Iebba V
AD  - Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo'.
AD  - Department of Medical Sciences, University of Trieste, Trieste.
FAU - Santinelli, Letizia
AU  - Santinelli L
AD  - Department of Public Health and Infectious Diseases.
FAU - Innocenti, Giuseppe P
AU  - Innocenti GP
AD  - Department of Public Health and Infectious Diseases.
FAU - Frasca, Federica
AU  - Frasca F
AD  - Laboratory of Virology, Department of Molecular Medicine, Istituto Pasteur 
      Italia, Sapienza University, Rome.
FAU - Bitossi, Camilla
AU  - Bitossi C
AD  - Laboratory of Virology, Department of Molecular Medicine, Istituto Pasteur 
      Italia, Sapienza University, Rome.
FAU - Scordio, Mirko
AU  - Scordio M
AD  - Laboratory of Virology, Department of Molecular Medicine, Istituto Pasteur 
      Italia, Sapienza University, Rome.
FAU - Oliveto, Giuseppe
AU  - Oliveto G
AD  - Laboratory of Virology, Department of Molecular Medicine, Istituto Pasteur 
      Italia, Sapienza University, Rome.
FAU - Ceccarelli, Giancarlo
AU  - Ceccarelli G
AD  - Department of Public Health and Infectious Diseases.
FAU - Antonelli, Guido
AU  - Antonelli G
AD  - Laboratory of Virology, Department of Molecular Medicine, Istituto Pasteur 
      Italia, Sapienza University, Rome.
AD  - Microbiology and Virology Unit, Sapienza University, Hospital Policlinico Umberto 
      I, Rome, Italy.
FAU - Mastroianni, Claudio Maria
AU  - Mastroianni CM
AD  - Department of Public Health and Infectious Diseases.
FAU - d'Ettorre, Gabriella
AU  - d'Ettorre G
AD  - Department of Public Health and Infectious Diseases.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (HLA-DR Antigens)
RN  - 0 (IFNAR1 protein, human)
RN  - 0 (MX1 protein, human)
RN  - 0 (Myxovirus Resistance Proteins)
RN  - 156986-95-7 (Receptor, Interferon alpha-beta)
RN  - 77238-31-4 (Interferon-beta)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - ADP-ribosyl Cyclase 1
MH  - Adult
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Case-Control Studies
MH  - Dysbiosis/*immunology
MH  - HIV Infections/*immunology/microbiology
MH  - HIV-1
MH  - HLA-DR Antigens
MH  - Humans
MH  - Interferon-beta/immunology
MH  - Lymphocyte Activation
MH  - Male
MH  - Middle Aged
MH  - Myxovirus Resistance Proteins/immunology
MH  - Pilot Projects
MH  - Prevotella/*isolation & purification
MH  - Receptor, Interferon alpha-beta/immunology
EDAT- 2020/07/18 06:00
MHDA- 2021/02/20 06:00
CRDT- 2020/07/18 06:00
PHST- 2020/07/18 06:00 [entrez]
PHST- 2020/07/18 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
AID - 00002030-202008010-00005 [pii]
AID - 10.1097/QAD.0000000000002574 [doi]
PST - ppublish
SO  - AIDS. 2020 Aug 1;34(10):1467-1473. doi: 10.1097/QAD.0000000000002574.