PMID- 32676173
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 14
IP  - 2
DP  - 2020
TI  - Comparative molecular cytogenetic characterization of five wild Vigna species 
      (Fabaceae).
PG  - 243-264
LID - 10.3897/CompCytogen.v14i2.51154 [doi]
AB  - To extend our knowledge on karyotype variation of the genus Vigna Savi, 1824, the 
      chromosomal organization of rRNA genes and fluorochrome banding patterns of five 
      wild Vigna species were studied. Sequential combined PI (propidium iodide) and 
      DAPI (4',6-diamidino-2-phenylindole) (CPD) staining and fluorescence in situ 
      hybridization (FISH) with 5S and 45S rDNA probes were used to analyze the 
      karyotypes of V. luteola (Jacquin, 1771) Bentham, 1959, V. vexillata (Linnaeus, 
      1753) A. Richard, 1845, V. minima (Roxburgh, 1832) Ohwi & H. Ohashi, 1969, V. 
      trilobata (Linnaeus, 1753) Verdcourt, 1968, and V. caracalla (Linnaeus, 1753) 
      Verdcourt,1970. For further phylogenetic analysis, genomic in situ hybridization 
      (GISH) with the genomic DNA of V. umbellata (Thunberg, 1794) Ohwi & H.Ohashi, 
      1969 onto the chromosomes of five wild Vigna species was also performed. Detailed 
      karyotypes were established for the first time using chromosome measurements, 
      fluorochrome bands, and rDNA-FISH signals. All species had chromosome number 2n = 
      2x = 22, and symmetrical karyotypes that composed of only metacentric or 
      metacentric and submetacentric chromosomes. CPD staining revealed all 45S rDNA 
      sites in the five species analyzed, (peri)centromeric GC-rich heterochromatin in 
      V. luteola, V. trilobata and V. caracalla, interstitial GC-rich and 
      pericentromeric AT-rich heterochromatin in V. caracalla. rDNA-FISH revealed two 
      5S loci in V. caracalla and one 5S locus in the other four species; one 45S locus 
      in V. luteola and V. caracalla, two 45S loci in V. vexillata and V. trilobata, 
      and five 45S loci in V. minima. The karyotypes of the studied species could be 
      clearly distinguished by the karyotypic parameters, and the patterns of the 
      fluorochrome bands and the rDNA sites, which revealed high interspecific 
      variation among the five species. The V. umbellata genomic DNA probe produced 
      weak signals in all proximal regions of V. luteola and all (peri)centromeric 
      regions of V. trilobata. The combined data demonstrate that distinct genome 
      differentiation has occurred among the five species during evolution. The 
      phylogenetic relationships between the five wild species and related cultivated 
      species of Vigna are discussed based on our present and previous molecular 
      cytogenetic data.
CI  - Chao-Wen She, Ying Mao, Xiang-Hui Jiang, Chun-Ping He.
FAU - She, Chao-Wen
AU  - She CW
AD  - Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of 
      Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua 
      University Huaihua China.
AD  - Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher 
      Education, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University 
      Huaihua China.
AD  - College of Biological and Food Engineering, Huaihua University, Huaihua, Hunan, 
      418008, China Huaihua University Huaihua China.
FAU - Mao, Ying
AU  - Mao Y
AD  - College of Biological and Food Engineering, Huaihua University, Huaihua, Hunan, 
      418008, China Huaihua University Huaihua China.
FAU - Jiang, Xiang-Hui
AU  - Jiang XH
AD  - Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of 
      Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua 
      University Huaihua China.
AD  - Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher 
      Education, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University 
      Huaihua China.
AD  - College of Biological and Food Engineering, Huaihua University, Huaihua, Hunan, 
      418008, China Huaihua University Huaihua China.
FAU - He, Chun-Ping
AU  - He CP
AD  - College of Chemistry and Material Engineering, Huaihua University, Huaihua, 
      Hunan, 418008, China Huaihua University Huaihua China.
LA  - eng
PT  - Journal Article
DEP - 20200626
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC7334243
OTO - NOTNLM
OT  - Vigna species
OT  - fluorescence in situ hybridization (FISH)
OT  - fluorochrome banding
OT  - karyotype
OT  - ribosomal RNA gene (rDNA)
EDAT- 2020/07/18 06:00
MHDA- 2020/07/18 06:01
PMCR- 2020/06/26
CRDT- 2020/07/18 06:00
PHST- 2020/02/15 00:00 [received]
PHST- 2020/04/15 00:00 [accepted]
PHST- 2020/07/18 06:00 [entrez]
PHST- 2020/07/18 06:00 [pubmed]
PHST- 2020/07/18 06:01 [medline]
PHST- 2020/06/26 00:00 [pmc-release]
AID - 51154 [pii]
AID - 10.3897/CompCytogen.v14i2.51154 [doi]
PST - epublish
SO  - Comp Cytogenet. 2020 Jun 26;14(2):243-264. doi: 10.3897/CompCytogen.v14i2.51154. 
      eCollection 2020.