PMID- 32683849
OWN - NLM
STAT- MEDLINE
DCOM- 20230222
LR  - 20230222
IS  - 1827-1669 (Electronic)
IS  - 0026-4806 (Linking)
VI  - 114
IP  - 1
DP  - 2023 Feb
TI  - Inhibiting MiR-34alpha reduces retinal cell apoptosis and downstream NF-kappaB pathway in 
      diabetic retinopathy rats through regulating HMGB1 expression.
PG  - 49-55
LID - 10.23736/S0026-4806.20.06625-2 [doi]
AB  - BACKGROUND: This is a research aimed to study the effect of micro ribonucleic 
      acid (miR)-34alpha on the retinal cell apoptosis in diabetic retinopathy (DR) rats 
      and its key molecular mechanism. METHODS: Sprague-Dawley rats were randomly 
      divided into healthy group (H group, N.=5), diabetes group (D group, N.=5), 
      diabetes + negative control transfection group (N group, N.=5) and diabetes + 
      miR-34alpha inhibitor transfection group (M group, N.=5). The rat model of diabetes 
      was established via intraperitoneal injection of 2% streptozotocin solution (60 
      mg/kg). After 72 h, the urine glucose and blood glucose were detected, and the 
      urine glucose above 3+ and the blood glucose concentration >16.7 mmol/L indicated 
      the successful modeling. After the rats were normally fed for 4 months, the 
      changes in expression of miR-34alpha in retinal tissues were detected via reverse 
      transcription-polymerase chain reaction (RT-PCR), the pathological changes in 
      retinal tissues were observed via hematoxylin-eosin (HE) staining, and the 
      retinal cell apoptosis was detected using terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay. Moreover, the changes 
      in the number of cells containing active caspase-3 in retinal tissues were 
      determined through immunohistochemistry, and the changes in expressions of 
      caspase-3, high mobility group box 1 (HMGB1) and nuclear factor-kappaB (NF-kappaB) in 
      retinal tissues were determined through Western blotting. RESULTS: Compared with 
      those in H group, the cell density declined, and the cells were arranged 
      disorderly with swelling in each retinal layer, the expression of miR-34alpha in 
      retinal tissues was increased, the retinal cell apoptosis was enhanced, the 
      number of cells containing active caspase-3 in retinal tissues rose, and the 
      expressions of caspase-3, HMGB1 and NF-kappaB in retinal tissues were increased in D 
      group, N group and M group (P<0.05). Compared with those in D group and N group, 
      the cell density rose, and the cells were arranged less disorderly with milder 
      swelling in each retinal layer, the expression of miR-34alpha in retinal tissues 
      declined, the retinal cell apoptosis was weakened, the number of cells containing 
      active caspase-3 in retinal tissues was decreased, and the expressions of 
      caspase-3, HMGB1 and NF-kappaB in retinal tissues were reduced in M group (P<0.05). 
      CONCLUSIONS: Inhibiting miR-34alpha reduces the retinal cell apoptosis in DR rats 
      through regulating the HMGB1 expression and downstream NF-kappaB pathway.
FAU - Ma, Yibin
AU  - Ma Y
AD  - Department of Ophthalmology, Taian City Central Hospital, Taian, China.
FAU - DU, Yunhong
AU  - DU Y
AD  - Department of Ophthalmology, Taian City Central Hospital, Taian, China.
FAU - Xu, Qian
AU  - Xu Q
AD  - Department of Ophthalmology, Taian City Central Hospital, Taian, China.
FAU - Bao, Huijing
AU  - Bao H
AD  - Department of Ophthalmology, Taian City Central Hospital, Taian, China.
FAU - Liu, Zhonglian
AU  - Liu Z
AD  - Radiotherapy Center, Taian Oncology Hospital, Taian, China.
FAU - Li, Yingchao
AU  - Li Y
AD  - Department of Ophthalmology, Taian City Central Hospital, Taian, China.
FAU - Liu, Wenjing
AU  - Liu W
AD  - Department of Ophthalmology, Taian City Central Hospital, Taian, China - 
      liuwenjing1977@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200717
PL  - Italy
TA  - Minerva Med
JT  - Minerva medica
JID - 0400732
RN  - 0 (NF-kappa B)
RN  - EC 3.4.22.- (Caspase 3)
RN  - 0 (Blood Glucose)
RN  - 0 (HMGB1 Protein)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Diabetic Retinopathy
MH  - NF-kappa B
MH  - Caspase 3
MH  - Blood Glucose
MH  - *HMGB1 Protein
MH  - Rats, Sprague-Dawley
MH  - Apoptosis
MH  - *MicroRNAs
MH  - *Diabetes Mellitus
EDAT- 2020/07/21 06:00
MHDA- 2023/02/22 06:00
CRDT- 2020/07/21 06:00
PHST- 2020/07/21 06:00 [pubmed]
PHST- 2023/02/22 06:00 [medline]
PHST- 2020/07/21 06:00 [entrez]
AID - S0026-4806.20.06625-2 [pii]
AID - 10.23736/S0026-4806.20.06625-2 [doi]
PST - ppublish
SO  - Minerva Med. 2023 Feb;114(1):49-55. doi: 10.23736/S0026-4806.20.06625-2. Epub 
      2020 Jul 17.