PMID- 32683850
OWN - NLM
STAT- MEDLINE
DCOM- 20230127
LR  - 20230202
IS  - 1827-1669 (Electronic)
IS  - 0026-4806 (Linking)
VI  - 113
IP  - 6
DP  - 2022 Dec
TI  - Melatonin relieves sepsis-induced myocardial injury via regulating JAK2/STAT3 
      signaling pathway.
PG  - 983-989
LID - 10.23736/S0026-4806.20.06626-4 [doi]
AB  - BACKGROUND: To study the effect of melatonin on myocardial injury of septic rats 
      through regulating the Janus kinase 2/signal transducer and activator of 
      transcription 3 (JAK2/STAT3) signaling pathway. METHODS: Healthy rats were 
      selected as the samples and divided into blank group, sepsis group and sepsis + 
      melatonin group. The difference in the myocardial tissue structure in the three 
      groups of rats was observed via hematoxylin-eosin (HE) staining, and the 
      messenger ribonucleic acid (mRNA) expression levels of JAK2 and STAT3 in 
      myocardium were compared among groups through fluorescence quantitative 
      polymerase chain reaction (qPCR). Western blotting was applied to detect the 
      protein expressions of JAK2, phosphorylated (p)-JAK2, STAT3 and p-STAT3, and 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      staining was utilized to determine the cell apoptosis in myocardial tissues. 
      Moreover, the activity of superoxide dismutase (SOD) and content of 
      malondialdehyde (MDA) in myocardial tissues as well as the activity of serum 
      creatine kinase (CK) and lactate dehydrogenase (LDH) were measured and compared. 
      RESULTS: There were inflammatory cells in the myocardium in sepsis group, and the 
      pathological changes were milder in sepsis + melatonin group. The mRNA 
      expressions of JAK2 and STAT3 and the protein expressions of JAK2, STAT3, p-JAK2 
      and p-STAT3 were raised remarkably in sepsis group compared with those in blank 
      group and sepsis + melatonin group. The apoptosis rate in tissues was 
      significantly different among the three groups, and it was the highest in sepsis 
      group, followed by that in sepsis + melatonin group and blank group. There were 
      significant differences in SOD activity and MDA content in myocardial tissues 
      among the three groups, of which the SOD activity was the strongest in blank 
      group, followed by that in sepsis + melatonin group and sepsis group, and the MDA 
      content was the highest in sepsis group, followed by that in sepsis + melatonin 
      group and blank group. Sepsis group had extremely notably increased activity of 
      CK and LDH compared with blank group, while sepsis + melatonin group exhibited 
      evidently decreased activity of CK and LDH in comparison with sepsis group. 
      CONCLUSIONS: The JAK2/STAT3 signaling pathway is associated with sepsis-induced 
      myocardial injury, and melatonin can relieve sepsis-induced myocardial injury by 
      regulating the JAK2/STAT3 signaling pathway.
FAU - Zhen, Genshen
AU  - Zhen G
AD  - Intensive Care Unit, Beijing Luhe Hospital, Capital Medical University, Beijing, 
      China - lhyyzgs@126.com.
FAU - Liang, Wen
AU  - Liang W
AD  - Department of Ultrasound, Beijing Luhe Hospital, Capital Medical University, 
      Beijing, China.
FAU - Jia, Huimiao
AU  - Jia H
AD  - Surgical Intensive Care Unit, Beijing Chao-yang Hospital, Capital Medical 
      University, Beijing, China.
FAU - Zheng, Xi
AU  - Zheng X
AD  - Surgical Intensive Care Unit, Beijing Chao-yang Hospital, Capital Medical 
      University, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200717
PL  - Italy
TA  - Minerva Med
JT  - Minerva medica
JID - 0400732
RN  - EC 2.7.10.2 (Jak2 protein, rat)
RN  - EC 2.7.10.2 (Janus Kinase 2)
RN  - JL5DK93RCL (Melatonin)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Rats
MH  - Apoptosis
MH  - *Heart Injuries
MH  - Janus Kinase 2/metabolism
MH  - *Melatonin/pharmacology
MH  - *Myocardial Reperfusion Injury/drug therapy
MH  - RNA, Messenger
MH  - *Sepsis/complications/drug therapy
MH  - Signal Transduction
MH  - STAT3 Transcription Factor
MH  - Superoxide Dismutase/metabolism
EDAT- 2020/07/21 06:00
MHDA- 2023/01/27 06:00
CRDT- 2020/07/21 06:00
PHST- 2020/07/21 06:00 [pubmed]
PHST- 2023/01/27 06:00 [medline]
PHST- 2020/07/21 06:00 [entrez]
AID - S0026-4806.20.06626-4 [pii]
AID - 10.23736/S0026-4806.20.06626-4 [doi]
PST - ppublish
SO  - Minerva Med. 2022 Dec;113(6):983-989. doi: 10.23736/S0026-4806.20.06626-4. Epub 
      2020 Jul 17.