PMID- 32685032
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220415
IS  - 1841-0987 (Print)
IS  - 1843-066X (Electronic)
IS  - 1841-0987 (Linking)
VI  - 16
IP  - 1
DP  - 2020 Jan-Mar
TI  - Quercetin and Isoquercitrin Inhibiting Hepatic Gluconeogenesis Through LKB1-AMPKalpha 
      Pathway.
PG  - 9-14
LID - 10.4183/aeb.2020.9 [doi]
AB  - OBJECTIVE: To observe the impact of quercetin and isoquercitrin on 
      gluconeogenesis in hepatocytes. METHODS: Mouse primary hepatocytes were cultured 
      with lactic acid and pyruvic acid. After treatment with quercetin and 
      isoquercitrin for 24 hours, the glucose concentration in the culture supernatant 
      was determined. RT-PCR was used to detect the mRNAs of PEPCK, G6Pase, LKB1, and 
      AMPKalpha. Protein levels of LKB1, AMPKalpha, and Thr172 phosphorylation were evaluated 
      by Western blot. RESULTS: The glucose concentration in the gluconeogenesis group 
      (GN) was significantly higher than in the control group (C), but the glucose 
      concentrations in the high level quercetin(group 80Q) and high level 
      isoquercitrin (group 80I) were significantly lower than in the group GN, P<0.01. 
      In the group 80Q, and group 80I, the mRNA levels of PEPCK and LKB1were 
      significantly lower than in the group GN (P<0.01), and the G6Pase mRNA were 
      significantly lower than in the group GN (P<0.05). The protein levels of LKB1 and 
      the phosphorylation of AMPKalpha Thr172 in the group 80Q, group 40I, and group 80I 
      were higher than in the group GN. The effects of quercetin and isoquercitrin on 
      LKB1 and AMPKalpha were similar to those of metformin. CONCLUSIONS: Quercetin and 
      isoquercitrin inhibit gluconeogenesis in hepatocytes, which may be related to the 
      LKB1 upregulation and phosphorylation of AMPKalpha.
CI  - (c)by Acta Endocrinologica Foundation.
FAU - Chen, L
AU  - Chen L
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Shen, T
AU  - Shen T
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Zhang, C P
AU  - Zhang CP
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Xu, B L
AU  - Xu BL
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Qiu, Y Y
AU  - Qiu YY
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Xie, X Y
AU  - Xie XY
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Wang, Q
AU  - Wang Q
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
FAU - Lei, T
AU  - Lei T
AD  - Shanghai University of Traditional Chinese Medicine, Putuo Hospital, Dept. of 
      Endocrinology, Shanghai, China.
LA  - eng
PT  - Journal Article
PL  - Romania
TA  - Acta Endocrinol (Buchar)
JT  - Acta endocrinologica (Bucharest, Romania : 2005)
JID - 101269720
PMC - PMC7363997
OTO - NOTNLM
OT  - AMP-activated protein kinase alpha (AMPKalpha)
OT  - gluconeogenesis
OT  - isoquercitrin
OT  - liver kinase B1 (LKB1)
OT  - quercetin
EDAT- 2020/07/21 06:00
MHDA- 2020/07/21 06:01
PMCR- 2020/07/01
CRDT- 2020/07/21 06:00
PHST- 2020/07/21 06:00 [entrez]
PHST- 2020/07/21 06:00 [pubmed]
PHST- 2020/07/21 06:01 [medline]
PHST- 2020/07/01 00:00 [pmc-release]
AID - aeb.2020.9 [pii]
AID - 10.4183/aeb.2020.9 [doi]
PST - ppublish
SO  - Acta Endocrinol (Buchar). 2020 Jan-Mar;16(1):9-14. doi: 10.4183/aeb.2020.9.