PMID- 32685762
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2451-8301 (Electronic)
IS  - 2451-8301 (Linking)
VI  - 9
DP  - 2020 Dec
TI  - Osmotic perception of GABAergic synaptic transmission in the supraoptic nucleus 
      of rats.
PG  - 58-64
LID - 10.1016/j.ibror.2020.06.007 [doi]
AB  - Extracellular osmolality plays a crucial role in controlling the activation of 
      neurons. Hypertonic stimulation modulates glutamatergic inputs to the supraoptic 
      nucleus (SON) magnocellular neurosecretory cells (MNCs) putative vasopressin (VP) 
      neurons through capsaicin-insensitive transient receptor potential vanilloid 
      (TRPV) 1 channels on the presynaptic terminals. However, it remains unclear 
      whether osmotic stimulation modulates GABAergic inputs to VP-secreting neurons 
      within punched-out slices containing only the SON and the perinuclear zone. To 
      answer this question, we studied the effects of various osmotic conditions on the 
      miniature GABAergic postsynaptic currents (mGPSCs) using the whole-cell 
      patch-clamp technique on rat SON putative VP-secreting neurons in small slice 
      preparations. We revealed that incubation in hypertonic solution for 2 h reduced 
      both the frequency and amplitude of the mGPSCs to the SON putative VP neurons, 
      whereas the mGPSCs were unaffected when the external osmolality was changed from 
      isotonic to hypotonic. Of interest, we found that changing from a hypertonic to 
      hypotonic environment increased the frequency of the mGPSCs. This effect was 
      independent of TRPV4. We hypothesize that two coordinated mechanisms may play an 
      important role in the regulation of a wide range of physiological functions of 
      VP.: 1) the modulation of GABA(A) receptor properties by brain-derived 
      neurotrophic factor (BDNF)-induced tyrosine kinase B receptor-mediated signaling 
      under hypertonic conditions, and 2) cell swelling-induced activation of 
      whole-cell anion currents under hypotonic conditions.
CI  - (c) 2020 The Author(s).
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, 
      University of Occupational and Environmental Health, Kitakyushu, Japan.
FAU - Saito, Takeshi
AU  - Saito T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
AD  - Department of Neurosurgery, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Yokoyama, Toru
AU  - Yokoyama T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Hashimoto, Hirofumi
AU  - Hashimoto H
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Maruyama, Takashi
AU  - Maruyama T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
FAU - Suzuki, Hideaki
AU  - Suzuki H
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, 
      University of Occupational and Environmental Health, Kitakyushu, Japan.
FAU - Ueta, Yoichi
AU  - Ueta Y
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, Japan.
LA  - eng
PT  - Journal Article
DEP - 20200702
PL  - England
TA  - IBRO Rep
JT  - IBRO reports
JID - 101691215
PMC - PMC7355382
OTO - NOTNLM
OT  - Hypertonic condition
OT  - Hypotonic stimulation
OT  - Miniature GABAergic postsynaptic currents (mGPSCs)
OT  - Supraoptic nucleus
OT  - Vasopressin neuron
OT  - Whole-cell patch-clamp
EDAT- 2020/07/21 06:00
MHDA- 2020/07/21 06:01
PMCR- 2020/07/02
CRDT- 2020/07/21 06:00
PHST- 2020/05/06 00:00 [received]
PHST- 2020/06/29 00:00 [accepted]
PHST- 2020/07/21 06:00 [entrez]
PHST- 2020/07/21 06:00 [pubmed]
PHST- 2020/07/21 06:01 [medline]
PHST- 2020/07/02 00:00 [pmc-release]
AID - S2451-8301(20)30022-4 [pii]
AID - 10.1016/j.ibror.2020.06.007 [doi]
PST - epublish
SO  - IBRO Rep. 2020 Jul 2;9:58-64. doi: 10.1016/j.ibror.2020.06.007. eCollection 2020 
      Dec.