PMID- 32687619
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1365-294X (Electronic)
IS  - 0962-1083 (Linking)
VI  - 29
IP  - 17
DP  - 2020 Sep
TI  - Daphnia magna modifies its gene expression extensively in response to caloric 
      restriction revealing a novel effect on haemoglobin isoform preference.
PG  - 3261-3276
LID - 10.1111/mec.15557 [doi]
AB  - Caloric restriction (CR) produces clear phenotypic effects within and between 
      generations of the model crustacean Daphnia magna. We have previously established 
      that micro-RNAs and cytosine methylation change in response to CR in this 
      organism, and we demonstrate here that CR has a dramatic effect on gene 
      expression. Over 6,000 genes were differentially expressed between CR and 
      well-fed D. magna, with a bias towards up-regulation of genes under caloric 
      restriction. We identified a highly expressed haemoglobin gene that responds to 
      CR by changing isoform proportions. Specifically, a transcript containing three 
      haem-binding erythrocruorin domains was strongly down-regulated under CR in 
      favour of transcripts containing fewer or no such domains. This change in the 
      haemoglobin mix is similar to the response to hypoxia in Daphnia, which is 
      mediated through the transcription factor hypoxia-inducible factor 1, and 
      ultimately the mTOR signalling pathway. This is the first report of a role for 
      haemoglobin in the response to CR. We also observed high absolute expression of 
      superoxide dismutase (SOD) in normally fed individuals, which contrasts with 
      observations of high SOD levels under CR in other taxa. However, key 
      differentially expressed genes, like SOD, were not targeted by differentially 
      expressed micro-RNAs. Whether the link between haemoglobin and CR occurs in other 
      organisms, or is related to the aquatic lifestyle, remains to be tested. It 
      suggests that one response to CR may be to simply transport less oxygen and lower 
      respiration.
CI  - (c) 2020 The Authors. Molecular Ecology published by John Wiley & Sons Ltd.
FAU - Hearn, Jack
AU  - Hearn J
AUID- ORCID: 0000-0003-3358-4949
AD  - Department of Vector Biology, Liverpool School of Tropical Medicine, Liverpool, 
      UK.
FAU - Clark, Jessica
AU  - Clark J
AD  - Institute of Biodiversity, Animal Health & Comparative Medicine, University of 
      Glasgow, Glasgow, UK.
FAU - Wilson, Philip J
AU  - Wilson PJ
AD  - School of Geosciences, University of Edinburgh, Edinburgh, UK.
FAU - Little, Tom J
AU  - Little TJ
AUID- ORCID: 0000-0002-8945-0416
AD  - Institute of Evolutionary Biology, School of Biological Sciences, University of 
      Edinburgh, Edinburgh, UK.
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200730
PL  - England
TA  - Mol Ecol
JT  - Molecular ecology
JID - 9214478
RN  - 0 (Hemoglobins)
RN  - 0 (Protein Isoforms)
SB  - IM
MH  - Animals
MH  - *Caloric Restriction
MH  - *Daphnia/genetics
MH  - Gene Expression
MH  - Hemoglobins/genetics
MH  - Protein Isoforms
OTO - NOTNLM
OT  - Daphnia
OT  - caloric restriction
OT  - ecological genomics
OT  - gene expression
OT  - methylation
OT  - micro-RNA
EDAT- 2020/07/21 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/07/21 06:00
PHST- 2019/11/12 00:00 [received]
PHST- 2020/07/03 00:00 [revised]
PHST- 2020/07/13 00:00 [accepted]
PHST- 2020/07/21 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/07/21 06:00 [entrez]
AID - 10.1111/mec.15557 [doi]
PST - ppublish
SO  - Mol Ecol. 2020 Sep;29(17):3261-3276. doi: 10.1111/mec.15557. Epub 2020 Jul 30.