PMID- 32690191
OWN - NLM
STAT- MEDLINE
DCOM- 20210304
LR  - 20240109
IS  - 1618-0984 (Electronic)
IS  - 0723-2020 (Linking)
VI  - 43
IP  - 4
DP  - 2020 Jul
TI  - Rhizobium ruizarguesonis sp. nov., isolated from nodules of Pisum sativum L.
PG  - 126090
LID - S0723-2020(20)30041-2 [pii]
LID - 10.1016/j.syapm.2020.126090 [doi]
AB  - Four strains, coded as UPM1132, UPM1133(T), UPM1134 and UPM1135, and isolated 
      from nodules of Pisum sativum plants grown on Ni-rich soils were characterised 
      through a polyphasic taxonomy approach. Their 16S rRNA gene sequences were 
      identical and showed 100% similarity with their closest phylogenetic neighbors, 
      the species included in the 'R. leguminosarum group': R. laguerreae FB206(T), R. 
      leguminosarum USDA 2370(T), R. anhuiense CCBAU 23252(T), R. sophoreae CCBAU 
      03386(T), R. acidisoli FH13(T) and R. hidalgonense FH14(T), and 99.6% sequence 
      similarity with R. esperanzae CNPSo 668(T). The analysis of combined housekeeping 
      genes recA, atpD and glnII sequences showed similarities of 92-95% with the 
      closest relatives. Whole genome average nucleotide identity (ANI) values were 
      97.5-99.7% ANIb similarity among the four strains, and less than 92.4% with 
      closely related species, while digital DNA-DNA hybridization average values 
      (dDDH) were 82-85% within our strains and 34-52% with closely related species. 
      Major fatty acids in strain UPM1133(T) were C18:1 omega7c / C18:1 omega6c in summed 
      feature 8, C14:0 3OH/ C16:1 iso I in summed feature 2 and C18:0. Colonies were 
      small to medium, pearl-white coloured in YMA at 28 degrees C and growth was observed in 
      the ranges 8-34 degrees C, pH 5.5-7.5 and 0-0.7% (w/v) NaCl. The DNA G+C content was 
      60.8mol %. The combined genotypic, phenotypic and chemotaxonomic data support the 
      classification of strains UPM1132, UPM1133(T), UPM1134 and UPM1135 into a novel 
      species of Rhizobium, for which the name Rhizobium ruizarguesonis sp. nov. is 
      proposed. The type strain is UPM1133(T) (=CECT 9542(T)=LMG 30526(T)).
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Jorrin, Beatriz
AU  - Jorrin B
AD  - Centro de Biotecnologia y Genomica de Plantas (CBGP), Universidad Politecnica de 
      Madrid - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, 
      28223 Pozuelo de Alarcon, Madrid, Spain.
FAU - Palacios, Jose Manuel
AU  - Palacios JM
AD  - Centro de Biotecnologia y Genomica de Plantas (CBGP), Universidad Politecnica de 
      Madrid - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, 
      28223 Pozuelo de Alarcon, Madrid, Spain; Departamento de Biotecnologia y Biologia 
      Vegetal, Escuela Tecnica Superior de Ingenieria Agronomica, Alimentaria y de 
      Biosistemas, Universidad Politecnica de Madrid, 28040 Madrid, Spain.
FAU - Peix, Alvaro
AU  - Peix A
AD  - Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de 
      Investigaciones Cientificas (IRNASA-CSIC), Salamanca, Spain; Unidad Asociada 
      Grupo de Interaccion Planta-Microorganismo Universidad de Salamanca-IRNASA 
      (CSIC). Electronic address: alvaro.peix@csic.es.
FAU - Imperial, Juan
AU  - Imperial J
AD  - Centro de Biotecnologia y Genomica de Plantas (CBGP), Universidad Politecnica de 
      Madrid - Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, 
      28223 Pozuelo de Alarcon, Madrid, Spain; Instituto de Ciencias Agrarias, Consejo 
      Superior de Investigaciones Cientificas (ICA-CSIC), 28006 Madrid, Spain. 
      Electronic address: juan.imperial@csic.es.
LA  - eng
PT  - Journal Article
DEP - 20200517
PL  - Germany
TA  - Syst Appl Microbiol
JT  - Systematic and applied microbiology
JID - 8306133
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Soil)
SB  - IM
MH  - DNA, Bacterial/genetics
MH  - Fatty Acids/analysis
MH  - Genes, Bacterial/genetics
MH  - Genome, Bacterial/genetics
MH  - Genotype
MH  - Nucleic Acid Hybridization
MH  - Pisum sativum/*microbiology
MH  - Phenotype
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rhizobium/chemistry/*classification/cytology/*physiology
MH  - Root Nodules, Plant/*microbiology
MH  - Sequence Analysis, DNA
MH  - Soil/chemistry
MH  - Soil Microbiology
MH  - Symbiosis
OTO - NOTNLM
OT  - Phylogeny
OT  - Pisum sativum
OT  - Rhizobium
OT  - Taxonomy
OT  - hydrogenase
OT  - legume endosymbiont
EDAT- 2020/07/22 06:00
MHDA- 2021/03/05 06:00
CRDT- 2020/07/22 06:00
PHST- 2020/04/16 00:00 [received]
PHST- 2020/04/22 00:00 [revised]
PHST- 2020/04/25 00:00 [accepted]
PHST- 2020/07/22 06:00 [entrez]
PHST- 2020/07/22 06:00 [pubmed]
PHST- 2021/03/05 06:00 [medline]
AID - S0723-2020(20)30041-2 [pii]
AID - 10.1016/j.syapm.2020.126090 [doi]
PST - ppublish
SO  - Syst Appl Microbiol. 2020 Jul;43(4):126090. doi: 10.1016/j.syapm.2020.126090. 
      Epub 2020 May 17.