PMID- 32691139
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20210609
IS  - 1432-2013 (Electronic)
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 472
IP  - 8
DP  - 2020 Aug
TI  - Propagation of spontaneous electrical activity in the ex vivo human uterus.
PG  - 1065-1078
LID - 10.1007/s00424-020-02426-w [doi]
AB  - Contractions of the non-pregnant uterus play a key role in fertility. Yet, the 
      electrophysiology underlying these contractions is poorly understood. In this 
      paper, we investigate the presence of uterine electrical activity and 
      characterize its propagation in unstimulated ex vivo human uteri. Multichannel 
      electrohysterographic measurements were performed in five freshly resected human 
      uteri starting immediately after hysterectomy. Using an electrode grid externally 
      and an electrode array internally, measurements were performed up to 24 h after 
      hysterectomy and compared with control. Up to 2 h after hysterectomy, we measured 
      biopotentials in all included uteri. The median root mean squared (RMS) values of 
      the external measurements ranged between 3.95 muV (interquartile range (IQR) 
      2.41-14.18 muV) and 39.4 muV (interquartile range (IQR) 10.84-105.64 muV) and were 
      all significantly higher than control (median RMS of 1.69 muV, IQR 1.13-3.11 muV), 
      consisting of chicken breast meat. The RMS values decreased significantly over 
      time. After 24 h, the median RMS (1.27 muV, IQR 0.86-3.04 muV) was comparable with 
      the control (1.69 muV, IQR 1.13-3.11 muV, p = 0.125). The internal measurements 
      showed a comparable pattern over time, but overall lower amplitude. The measured 
      biopotentials propagated over the uterine surface, following both a plane-wave as 
      well as an erratic pattern. No clear pacemaker location nor a preferred 
      propagation direction could be identified. These results show that ex vivo uteri 
      can spontaneously generate propagating biopotentials and provide novel insight 
      contributing to improving our understanding of the electrophysiology of the human 
      non-pregnant uterus.
FAU - Kuijsters, Nienke P M
AU  - Kuijsters NPM
AUID- ORCID: 0000-0001-9182-8767
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands. nienkekuijsters@live.nl.
AD  - Department of Obstetrics and Gynaecology, Catharina Hospital, Michelangelolaan 2, 
      5623 EJ, Eindhoven, the Netherlands. nienkekuijsters@live.nl.
FAU - Sammali, Federica
AU  - Sammali F
AUID- ORCID: 0000-0002-5434-5384
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands.
FAU - Ye, Xin
AU  - Ye X
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands.
FAU - Blank, Celine
AU  - Blank C
AUID- ORCID: 0000-0001-5889-8560
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands.
AD  - Department of Obstetrics and Gynaecology, University Hospital (UZ) Gent, C. 
      Heymanslaan 10, 9000, Ghent, Belgium.
FAU - Xu, Lin
AU  - Xu L
AUID- ORCID: 0000-0001-5777-1496
AD  - School of Information Science and Technology, ShanghaiTech University, Shanghai, 
      China.
FAU - Mischi, Massimo
AU  - Mischi M
AUID- ORCID: 0000-0002-1179-5385
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands.
FAU - Schoot, Benedictus C
AU  - Schoot BC
AUID- ORCID: 0000-0002-0517-7126
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands.
AD  - Department of Obstetrics and Gynaecology, Catharina Hospital, Michelangelolaan 2, 
      5623 EJ, Eindhoven, the Netherlands.
AD  - Department of Obstetrics and Gynaecology, University Hospital (UZ) Gent, C. 
      Heymanslaan 10, 9000, Ghent, Belgium.
FAU - Rabotti, Chiara
AU  - Rabotti C
AUID- ORCID: 0000-0001-8865-1219
AD  - Department of Electrical Engineering (Signal Processing Systems: Biomedical 
      Diagnostics), Eindhoven Technical University, Post box 513, 5600 MB, Eindhoven, 
      the Netherlands.
LA  - eng
SI  - NTR/NTR5264
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200720
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
SB  - IM
CIN - Pflugers Arch. 2020 Aug;472(8):989. PMID: 32617653
MH  - Animals
MH  - Chickens/physiology
MH  - Female
MH  - Humans
MH  - Meat
MH  - Uterus/*physiology
PMC - PMC7376519
OTO - NOTNLM
OT  - Fertility
OT  - Pacemaker
OT  - Propagation
OT  - Smooth muscle
OT  - Uterine contractility
OT  - Uterine electrophysiology
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/07/22 06:00
MHDA- 2021/06/10 06:00
PMCR- 2020/07/20
CRDT- 2020/07/22 06:00
PHST- 2020/01/11 00:00 [received]
PHST- 2020/06/26 00:00 [accepted]
PHST- 2020/05/21 00:00 [revised]
PHST- 2020/07/22 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
PHST- 2020/07/22 06:00 [entrez]
PHST- 2020/07/20 00:00 [pmc-release]
AID - 10.1007/s00424-020-02426-w [pii]
AID - 2426 [pii]
AID - 10.1007/s00424-020-02426-w [doi]
PST - ppublish
SO  - Pflugers Arch. 2020 Aug;472(8):1065-1078. doi: 10.1007/s00424-020-02426-w. Epub 
      2020 Jul 20.