PMID- 32692538 OWN - NLM STAT- MEDLINE DCOM- 20210514 LR - 20240329 IS - 1936-086X (Electronic) IS - 1936-0851 (Print) IS - 1936-0851 (Linking) VI - 14 IP - 8 DP - 2020 Aug 25 TI - Dextran-Coated Cerium Oxide Nanoparticles: A Computed Tomography Contrast Agent for Imaging the Gastrointestinal Tract and Inflammatory Bowel Disease. PG - 10187-10197 LID - 10.1021/acsnano.0c03457 [doi] AB - Computed tomography (CT) is an X-ray-based medical imaging technique commonly used for noninvasive gastrointestinal tract (GIT) imaging. Iodine- and barium-based CT contrast agents are used in the clinic for GIT imaging; however, inflammatory bowel disease (IBD) imaging is challenging since iodinated and barium-based CT agents are not specific for sites of inflammation. Cerium oxide nanoparticles (CeNP) can produce strong X-ray attenuation due to cerium's k-edge at 40.4 keV but have not yet been explored for CT imaging. In addition, we hypothesized that the use of dextran as a coating material on cerium oxide nanoparticles would encourage accumulation in IBD inflammation sites in a similar fashion to other inflammatory diseases. In this study, therefore, we sought to develop a CT contrast agent, i.e., dextran-coated cerium oxide nanoparticles (Dex-CeNP) for GIT imaging with IBD. We synthesized Dex-CeNP, characterized them using various analytical tools, and examined their in vitro biocompatibility, CT contrast generation, and protective effect against oxidative stress. In vivo CT imaging was done with both healthy mice and a dextran sodium sulfate induced colitis mouse model. Dex-CeNP's CT contrast generation and accumulation in inflammation sites were compared with iopamidol, an FDA approved CT contrast agent. Dex-CeNP was found to be protective against oxidative damage. Dex-CeNP produced strong CT contrast and accumulated in the colitis area of large intestines. In addition, >97% of oral doses were cleared from the body within 24 h. Therefore, Dex-CeNP can be used as a potential CT contrast agent for imaging GIT with IBD while protecting against oxidative damage. FAU - Naha, Pratap C AU - Naha PC AUID- ORCID: 0000-0002-3173-7879 AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Hsu, Jessica C AU - Hsu JC AUID- ORCID: 0000-0002-3599-2834 AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. AD - Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Kim, Johoon AU - Kim J AUID- ORCID: 0000-0002-1138-5984 AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. AD - Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Shah, Shrey AU - Shah S AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. AD - Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Bouche, Mathilde AU - Bouche M AUID- ORCID: 0000-0003-2707-1290 AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Si-Mohamed, Salim AU - Si-Mohamed S AD - Department of Radiology, Hopital Cardio-Vasculaire et Pneumologique Louis Pradel, Lyon 69500, France. AD - Centre de Recherche en Acquisition et Traitement de l'Image pour la Sante (CREATIS), UMR CNRS 5220, Inserm U1044, University Lyon1 Claude Bernard, Lyon 69621, France. FAU - Rosario-Berrios, Derick N AU - Rosario-Berrios DN AD - Department of Biochemistry and Molecular Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Douek, Philippe AU - Douek P AD - Department of Radiology, Hopital Cardio-Vasculaire et Pneumologique Louis Pradel, Lyon 69500, France. AD - Centre de Recherche en Acquisition et Traitement de l'Image pour la Sante (CREATIS), UMR CNRS 5220, Inserm U1044, University Lyon1 Claude Bernard, Lyon 69621, France. FAU - Hajfathalian, Maryam AU - Hajfathalian M AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Yasini, Parisa AU - Yasini P AUID- ORCID: 0000-0001-8072-6597 AD - Department of Chemistry, Temple University, Philadelphia, Pennsylvania 19122, United States. FAU - Singh, Sanjay AU - Singh S AUID- ORCID: 0000-0002-6256-7091 AD - Division of Biological and Life Sciences School of Arts and Sciences Ahmedabad University, Ahmedabad, Gujarat 380009, India. FAU - Rosen, Mark A AU - Rosen MA AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Morgan, Matthew A AU - Morgan MA AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. FAU - Cormode, David P AU - Cormode DP AUID- ORCID: 0000-0002-8391-9500 AD - Department of Radiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. AD - Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. AD - Department of Medicine, Division of Cardiovascular Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States. LA - eng GR - R01 HL131557/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. DEP - 20200728 PL - United States TA - ACS Nano JT - ACS nano JID - 101313589 RN - 0 (Contrast Media) RN - 0 (Dextrans) RN - 30K4522N6T (Cerium) RN - 619G5K328Y (ceric oxide) SB - IM MH - Animals MH - *Cerium MH - *Colitis/chemically induced/diagnostic imaging MH - Contrast Media MH - Dextrans MH - *Inflammatory Bowel Diseases/diagnostic imaging/drug therapy MH - Mice MH - *Nanoparticles PMC - PMC7484129 MID - NIHMS1614765 OTO - NOTNLM OT - cerium oxide OT - colitis OT - computed tomography OT - contrast agent OT - nanoparticles EDAT- 2020/07/22 06:00 MHDA- 2021/05/15 06:00 PMCR- 2021/08/25 CRDT- 2020/07/22 06:00 PHST- 2020/07/22 06:00 [pubmed] PHST- 2021/05/15 06:00 [medline] PHST- 2020/07/22 06:00 [entrez] PHST- 2021/08/25 00:00 [pmc-release] AID - 10.1021/acsnano.0c03457 [doi] PST - ppublish SO - ACS Nano. 2020 Aug 25;14(8):10187-10197. doi: 10.1021/acsnano.0c03457. Epub 2020 Jul 28.