PMID- 32693566
OWN - NLM
STAT- MEDLINE
DCOM- 20201103
LR  - 20201103
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 34
IP  - 3
DP  - 2020 May-Jun
TI  - Desflurane pretreatment can reduce sepsis-evoked lung injury in rats via 
      inhibiting STAT3 pathway.
PG  - 935-942
LID - 10.23812/20-173-A-48 [doi]
AB  - The purpose of this study was to investigate the effect of desflurane (Des) 
      pretreatment on sepsisevoked lung injury in rats and its mechanism. The rat model 
      of sepsis-evoked lung injury was prepared using lipopolysaccharide (LPS), while 
      rat lung mesenchymal cell (MSC) model was cultured in vitro, followed by Des 
      pretreatment or inhibitor S31-201 culture. The degree of lung tissue injury was 
      analyzed by Hematoxylin-eosin (HE) staining. The expression levels of interleukin 
      (IL)-6, IL-1beta and tumor necrosis factor (TNF)-alpha in the serum of rats were 
      detected by enzyme-linked immunosorbent assay (ELISA). One-step terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was 
      utilized to determine the apoptosis levels of lung tissues and MSCs cultured in 
      vitro. The expressions of the signal transducer and activator of transcription 3 
      (STAT3) pathway in rat lung tissues and MSCs were detected by Western blotting. 
      After modeling, LPS induced the lung injury in rats, the expression levels of 
      IL-6, IL-1beta and TNF-alpha were up-regulated (P<0.05), the apoptosis rate was 
      increased (P<0.05), and phosphorylated-Janus kinase 2 (p-JAK2) and 
      phosphorylated-STAT3 (p-STAT3) protein expressions were up-regulated (P<0.05). 
      Des pretreatment can alleviate LPS-induced lung injury, down-regulate IL-6, IL-1beta 
      and TNF-alpha expression levels (P<0.05), reduce apoptosis (P<0.05), and downregulate 
      p-JAK2 and p-STAT3 protein levels (P<0.05). LPS induced an increase in apoptosis 
      rate of MSCs (P<0.05) and the up-regulation of p-STAT3 protein expression 
      (P<0.05). Both Des pretreatment and S31-201 inhibitor culture could reduce the 
      apoptosis rate (P<0.05) and down-regulate p-STAT3 protein level (P<0.05). Des 
      pretreatment can reduce sepsis-evoked lung injury in rats, which may be related 
      to the inhibition of protein expressions of STAT3 pathway.
CI  - Copyright 2020 Biolife Sas. www.biolifesas.org.
FAU - Wang, C
AU  - Wang C
AD  - Department of Anesthesiology, The Second Hospital of Dalian Medical University, 
      Dalian, Liaoning, China.
FAU - Liu, N
AU  - Liu N
AD  - Department of Anesthesiology, The Second Hospital of Dalian Medical University, 
      Dalian, Liaoning, China.
FAU - Yang, H T
AU  - Yang HT
AD  - Department of Anesthesiology, The Second Hospital of Dalian Medical University, 
      Dalian, Liaoning, China.
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Interleukin-6)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, rat)
RN  - CRS35BZ94Q (Desflurane)
SB  - IM
MH  - Animals
MH  - Desflurane
MH  - Interleukin-6
MH  - *Lung Injury
MH  - Rats
MH  - STAT3 Transcription Factor
MH  - *Sepsis
OTO - NOTNLM
OT  - STAT3 pathway
OT  - desflurane
OT  - lung injury
OT  - rats
OT  - sepsis
EDAT- 2020/07/23 06:00
MHDA- 2020/11/04 06:00
CRDT- 2020/07/23 06:00
PHST- 2020/07/23 06:00 [entrez]
PHST- 2020/07/23 06:00 [pubmed]
PHST- 2020/11/04 06:00 [medline]
AID - 11 [pii]
AID - 10.23812/20-173-A-48 [doi]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2020 May-Jun;34(3):935-942. doi: 
      10.23812/20-173-A-48.